Fig. 2

Therapeutic potentials of Klotho-enhanced UDSCs. A Klotho-enhanced UDSCs (PKH26: Red) were intravenously administered to both wild-type (Sham) and ischemia–reperfusion injury (IRI) groups. B Klotho expression (Klotho: Green) was detected in the renal cortex region after Klotho-enhanced UDSCs administration. C The images of A and B have been quantitatively analyzed. D Tissue injury was assessed using H&E staining. Renal fibrosis was measured using MT staining and type IV Collagen expression. E Kidney injury was represented by urine NGAL levels and was normalized using urine creatinine levels. Results were shown as the mean ± SEM. **p < 0.01, ****p < 0.0001. H&E, hematoxylin and eosin; MT, Masson’s trichrome; NGAL, neutrophil gelatinase associated lipocalin