Fig. 4
From: Short cell cycle duration is a phenotype of human epidermal stem cells
Single cell RNA sequencing shows a decrease in both G1 and G2M phases in human stem cells vs. committed progenitors. (A) Umap of INVOLUCRIN/ LORICRIN/ FILAGGRIN negative keratinocytes. Cells in cluster 9 express high MKI67. (B) Violin plot for populations in (A). (C) Subclusters of 3 putative progenitor clusters. (D) Violin plot for populations in (C). (E) Cell cycle distribution of holoclones vs. meroclones vs. paraclones using tricycle. (F) Inferred differences in cell cycle phase length from (E) above, based on the ergodic principle and assumption of equivalent S phase duration. (G) Data analysis combining cell phase distribution from Fig. 4E with the median CCDs of EXP vs. DIF colonies from live cell imaging. Data presented as mean ± SEM. Not significant (ns) P > 0.05. *P < 0.05. **P < 0.01