Fig. 7

Dissociated human:human retinal organoid co-cultures cluster more closely together than human:mouse co-cultures. Week 20 retinal organoids and mouse retinal dissociates were cultured on PDL and laminin coated glass dishes for live imaging. Representative images after 3 DIV of A Human retinal organoid co-culture from CRX-tdTomato H9-derived retinal dissociates and Nrl-eGFP H9 derived retinal dissociates and Nrl::GFP mouse retinal dissociates co-cultured with human CRX-tdTomato H9 derived retinal organoid dissociates. Scale bar = 20 μm. B Schematic of quantifications for nearest neighbour and neurite length. C Nearest neighbour analysis was performed to quantify the average distance of the nearest neighbour between tdTomato and eGFP cells, analyzed by unpaired, two-tailed t test. *p < 0.05. D Average neurite length in human:human and human:mouse co-cultures. Data is presented as mean ± SEM (n = 3 biological replicates), analyzed by 2-way ANOVA with Sidak’s post-hoc test. **p < 0.01