Fig. 4

Gene expression of monocytes educated with EV or LPS-EV produced by flask or bioreactor process. Monocytes from 3 isolates were educated with flask (F_1) or bioreactor (B_1) produced EVs or LPS-EVs flask to generate flask EEMos, flask LPS-EEMos, bioreactor EEMos, or bioreactor LPS-EEMos After education, monocytes were collected, RNA isolated and analyzed by RT-PCR for gene expression (N = 3 to 6 biological replicates for each isolate). The fold-change of gene expression (± SEM) normalized to a GAPDH housekeeping gene and compared to untreated control monocytes A IL-6, B IL-8, IDO, FGF2 and C IL-7, IL-10, IL-12, and IL-15. Groups compared by Kruskal–Wallis with a Dunn’s post-test, *p < / = 0.05, ** p < / = 0.005, *** p < / = 0.0005 **** p < / = 0.0001