Fig. 6
Treatment with EVs or LPS-EVs from flask or bioreactor in mice after lethal ARS. On day 0, NSG mice received 4 Gy of lethal radiation followed by an i.v. treatment 4 h later with vehicle control (PBS), or 5 × 10 9 of flask-EVs, flask LPS-EVs (24H flask or 24H + LPS flask) from the F_1 MSC isolate, bioreactor-EVs, or bioreactor LPS-EVs (96H Bioreactor or 24H + LPS Bioreactor) from the B MSC isolate. Mice were followed for A overall survival, B clinical scores (percent weight loss, posture, activity, and fur texture) and C percent weight change. The final mean percent weight change and clinical score were carried over after death to allow for comparison by Kruskal–Wallis with a Dunn post-test between groups at a given time point. Results pooled from three separate experiments with 4 to 12 mice/group. *p < .05, ***p ≤ .005, ****p ≤ .0001