Fig. 2

Histological characteristics of the hiPSC-CM patch. a: Image of a hiPSC-CM patch in a 6-cm diameter dish. b: Hematoxylin and eosin staining of hiPSC-CM patches created on a 24-well plate. Scale bars: 50 μm (b). c, d: Representative image of immunostained hiPSC-CM patches created on a 24-well plate. c TnT (green) and actinin (red); d TnT (red) and α-MHC, β-MHC, MLC2v, MLC2a, connexin 43 (cx43), N-cadherin, collagen I, and laminin (green). Scale bars: 20 μm, 10 μm (inset image). e: In vitro quantification of cytokines and growth factors. The culture supernatant of the hiPSC-CM patches under normoxic conditions (n = 5) was collected and assessed using the Bioplex suspension array system. f: Electrophysiological properties of the hiPSC-CM patch created on a 96-well plate. Extracellular field potentials were recorded using a multielectrode array system. A representative extracellular potential waveform and a propagation map are shown. g: The contractile force of the hiPSC-CM patch created on a 96-well plate was assessed using MicroTester G2. The relationship between the contraction force and the stretch rate is shown