Fig. 1

Sensitivity to CPZ and BAs in cholangiocyte-like cell organoids (CLCOs). A The morphology of CLCOs incubated with different concentrations of chlorpromazine (0, 30, 50, 80 and 160 μM) for 24, 48 and 72 h. Scale bar = 100 µm. B, C CLCOs were exposed to a concentration range of CPZ (0, 10, 20, 30, 40, 50, 80, 160 and 320 μM) with non-cholestatic or cholestatic conditions (presence or absence of a BA cocktail (BA 40× or 80×)) for 24 and 72 h. Cell viability was measured by AlamarBlue reagent (B), and cytotoxicity was measured by the LDH leakage assay (C). Each data point represents the mean ± SEM viability of n = 5 independent donors