Fig. 3
Effects of CPZ and BA cocktail on intracellular generation of reactive oxygen species (ROS) and expression of oxidative stress-related genes. A H2DCFDA assay in CLCOs. CLCOs were pretreated with 30 μM CPZ with or without BA cocktail (BA 40× or 80×), and 500 μM H2O2 as positive control for 2 h. Data are presented as mean ± SD of five independent donors. Statistical differences between groups were tested using one-way ANOVA followed by Tukey’s test for multiple comparisons; *P < 0.05, **P < 0.01, ***P < 0.001. B, C GSH/GSSG (B), total GSH (GSH + 2 GSSG) (C) were measured in CLCOs. CLCOs were pretreated with different conditions (30 μM CPZ with or without BA cocktail (BA 40× or 80×)) for 24 h. Data are presented as mean ± SD of five independent donors. Statistical differences between groups were tested using one-way ANOVA followed by Dunn’s test for multiple comparisons; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. D Gene expression of oxidative stress-related genes. CLCOs were pretreated with different conditions (30 μM CPZ with or without BA cocktail (BA 40× or 80×)) for 24 h. Gene expression was measured by qPCR and normalized to the housekeeping genes. Each data point represents the mean ± SD of five independent donors. Statistical differences between groups were tested using one-way ANOVA followed by Dunn’s test for multiple comparisons; *P < 0.05, **P < 0.01