Fig. 4

Effects of CPZ and BA cocktail on barrier function. A Gene expression of the barrier function-related genes. CLCOs were pretreated with 30 μM CPZ with or without BA cocktail (BA 40× or 80×) for 24 h. Gene expression was measured by qPCR and normalized to the housekeeping genes. Data are presented as mean ± SD of five independent donors. Statistical differences between groups were tested using one-way ANOVA followed by Tukey’s test for multiple comparisons; *P < 0.05, **P < 0.01. B, C CLCOs barrier disruption assessed by immunofluorescence after CPZ exposure of 24 h (B) and 72 h (C). Scale bar = 50 µm