Fig. 5

Trans-epithelial barrier function. A Fluorescent images show the barrier function by intraluminal fluorescence intensity. Scale bar = 1000 µm. B Mean intraluminal fluorescence intensity normalized to background levels for CLCOs. Data are presented as mean ± SD for each group (N = 150 organoids). Statistical differences between groups were tested using one-way ANOVA followed by Dunn’s test for multiple comparisons; ns indicate no significant differences; ****P < 0.0001