Fig. 6

Effects of CPZ and TNFα in proinflammatory response. A Gene expression of the proinflammatory cytokine-related genes. CLCOs were pretreated with 30 μM CPZ and/or 30 ng/mL TNFα for 24 h. Gene expression was measured by qPCR and normalized to the housekeeping genes. Data are presented as mean ± SD of five independent donors. Statistical differences between groups were tested using paired t test; *P < 0.05. B Cytotoxicity of CPZ-TNFα. CLCOs were pretreated with 30 μM CPZ and/or 30 ng/mL TNFα for 24 h. Cytotoxicity was measured by the LDH assay. Data are presented as mean ± SD of five independent donors. Statistical differences between groups were using one-way ANOVA followed by Dunn’s test for multiple comparisons; *P < 0.05