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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Administration of adipose-derived stem cells extracellular vesicles in a murine model of spinal muscular atrophy: effects of a new potential therapeutic strategy

Fig. 3

Effect of ASC-EVs administration on lumbar MNs degeneration in SMA mice. (A) The graph shows the quantification of MNs density (MNs number/volume) in the ventral horns of L1-L5 spinal cord for SMA-PBS (white), SMA-EVs (grey) and WT (black) group at P10. The treatment with ASC-EVs significantly protect MNs from neurodegeneration compared to control mice (One-way ANOVA ***p < 0.0010; ****p < 0.0001). (B) Representative Nissl stained sections of lumbar spinal cord of SMA-PBS, SMA-EVs and WT mice. The arrows show stained MNs. Scale bar 500 μm. (C) The graph shows the quantification of the percentage of MNs (SMI32 + cells) expressing Casp3 in SMA-PBS (white), SMA-EVs (grey) and WT (black) groups: the treatment with ASC-EVs decreased the activation of apoptotic marker Casp3 compared to control mice (One-way ANOVA **p = 0.0050). (D) Representative confocal images showing Casp3+ (red) and SMI32+ (green) cells in the ventral horns of PBS- and ASC-EVs treated SMA and WT mice. Cell nuclei are labelled by DAPI staining (blue). The arrows show Casp3+/SMI32 + cells. Scale bar 50 μm

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Stem Cell Research & Therapy

ISSN: 1757-6512