Fig. 2

Electrophysiological characterization of Anatomic-derived neurons (Ctrl1 and Ctrl2). A Comparison of percentage mature neurons (first AP generated with a square pulse current injection with steps of 10pA) between clump and single cell seeding. i. Representative mature and immature AP traces ii. Percentage of mature neurons: Clump Ctrl1- DIV14: 60% (n = 3 out of 5), DIV28: 100% (n = 5 out of 5), DIV35: 83% (n = 5 out of 6) and Single cell Ctrl1- DIV14: 20% (n = 1 out of 5), DIV28: 100% (n = 5 out of 5), DIV35: 100% (n = 6 out of 6). Ctrl2—Percentage of mature neurons: DIV14: (67% (n = 4 out of 6), DIV21: 71% (n = 5 out of 7), DIV28: 100% (n = 7 out of 7) and DIV35: 100% (n = 5 out of 5). Number of mature cells out of total cells patched are denoted for each recording day above the bar. B RMP of Ctrl1 and Ctrl2 neurons. C Cell capacitance (pF) for Ctrl1 and Ctrl2 neurons. D TTXr currents recorded in the presence of 500 nM TTX. i. Voltage protocol, ii. Representative current traces, iii. Current density measured on DIV14, 28 and 35. E Voltage clamp recordings of inward sodium and outward potassium currents. i. Voltage protocol ii. Na⁺ and K⁺ current traces. iii. Sodium current density measured on DIV14 and 28 of maturation. iv. Potassium current density measured on DIV14 and 28 of maturation. F Tonic firing in response to ramp current stimuli i. Ramp current stimuli, ii. AP firing in response to ramp current stimuli 1nA/1 s. iii Percentage tonic neurons. Data shown as mean ± SEM. One-way Anova Bonferroni's multiple comparisons test