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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: How to differentiate induced pluripotent stem cells into sensory neurons for disease modelling: a functional assessment

Fig. 3

Anatomic protocol: Differentiation and Maturation (Ctrl2). A Differentiation: Phase contrast images display single cell seeding of iPSCs on DIV0 of differentiation and generation of immature neurons by DIV7. Scale bar—200 µm. B Maturation: Maturation of neurons DIV7-35. Differentiation resulted in a homogenous neuronal network during the maturation period. Scale Bar 200 µm. C Immunostaining of neurons for Peripherin and Tuj1 on DIV8, 14, 21, 28 and 35. Scale Bar—100 µm. DIV-Days in vitro. Peripherin-green, Tuj1-red and DAPI-blue fluorescence

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