Fig. 3From: How to differentiate induced pluripotent stem cells into sensory neurons for disease modelling: a functional assessmentAnatomic protocol: Differentiation and Maturation (Ctrl2). A Differentiation: Phase contrast images display single cell seeding of iPSCs on DIV0 of differentiation and generation of immature neurons by DIV7. Scale bar—200 µm. B Maturation: Maturation of neurons DIV7-35. Differentiation resulted in a homogenous neuronal network during the maturation period. Scale Bar 200 µm. C Immunostaining of neurons for Peripherin and Tuj1 on DIV8, 14, 21, 28 and 35. Scale Bar—100 µm. DIV-Days in vitro. Peripherin-green, Tuj1-red and DAPI-blue fluorescenceBack to article page