Fig. 4

Chambers protocol: Differentiation and Maturation (Ctrl1). A Phase contrast images during the differentiation and maturation of iPSC-derived from Ctrl1 subject. DIV0 iPSCs, DIV10 generation of immature neurons, DIV35 shows ganglion-like morphology (marked with blue arrows). DIV18 and 35 also shows the culture outgrown with other non-neuronal cell types (marked with red arrows). Scale bar—200 µm. B Neurons at DIV35 from Anatomic protocol results in homogenous neuronal network. Scale bar—200 µm. C Immunostaining confirms peripheral neuronal identity of neurons from DIV11, 14, 21, 28 and 35 of maturation. DIV-Days in vitro. Scale bar − 100 µm. Peripherin-green, Tuj1-red and DAPI-blue fluorescence