Fig. 5

Comparison of electrophysiological characteristics of Anatomic and Chambers-derived neurons (Ctrl1). A Comparison of percentage of mature APs (First AP generated with a square pulse current injection) Percentage Mature neurons- DIV14: 43% (n = 2 out of 7), DIV28: 100% (n = 5 out of 5) and DIV35: 100% (n = 5 out of 5) for neurons generated with Chambers protocol. Number of cells having APs with overshoot above 0 mV out of total cells patched are denoted for each recording day above the bar. B RMP measured from both the protocols. One way Anova Bonferroni's multiple comparisons test. C Cell capacitance (pF) measured from both the protocols. One way Anova Bonferroni's multiple comparisons test. D TTXr currents recorded in the presence of 500 nM TTX. Mann–Whitney test. E, F Voltage clamp recordings of inward Na⁺ and K⁺ current density measured on DIV14 of maturation. Mann–Whitney test G Tonic firing neurons in response to ramp current stimuli. Chambers protocol- DIV28: n = 4 (4/4 Tonic, 1 cell no AP), DIV35: n = 5 (5/5 Tonic). H The average number of APs generated in response to ramp current stimuli. n = 5 for both protocols. Multiple t test. DIV-Days in vitro. Data are shown as mean ± SEM