Fig. 8

Anatomic RealDRG™ can be adapted for FLIPRPenta high-throughput Ca²⁺ assays. A Ca²⁺ responses of sensory neurons at DIV15 plated with varying cell densities (1000–10,000 cells/well) on 384-well plates. B KCl-induced Ca²⁺ responses at DIV15, DIV21 and DIV35 C sample trace from DIV21. D Veratridine-induced Ca²⁺ responses at DIV15, 21 and 35 E sample trace showing complete inhibition of responses by TTX (1 µM). F Capsaicin-induced Ca²⁺ responses emerge at DIV35, G capsaicin sample trace. H 24 h treatment with IL6/soluble IL6-R does not affect the response properties of sensory neurons at DIV35. Data are presented as mean ± S.D. from n > 3 replicates