Fig. 8From: Expression profiling of stemness markers in testicular germline stem cells from neonatal and adult Swiss albino mice during their transdifferentiation in vitroExpression of NESTIN (A and E), TBXT (BRACHYURY) (B and F), and PDX-1 (C and G) embryoid bodies (EBs) derived from adult mice testicular cells 10 days after removal of LIF and GDNF, (D and H) are secondary antibody control. Bar = 20 μm. Real time PCR analysis of germ layer markers Nes, Des, Afp in SSC-derived EBs 10 days after removal of LIF and GDNF (I). Actb was used for internal normalization and the expression levels of these genes in EBs were further normalized with their corresponding expression levels in SSCsBack to article page