Table 1 Sequences of primers used in the real time and RT-PCR experiment
Serial No. | Gene Name | Primer Name | Primer Sequences (5’ – 3’) |
|---|---|---|---|
1. | β-actin (Actb) | ba-560f | CTACCTCATGAAGATCCTGA |
ba-619r | TGATGTCACGCACGATTT | ||
2. | Alkaline phosphatase2 (Alpl) | akp2-1089f | CGCCATGACATCCCAGAAA |
akp2-1168r | GGGTGTATCCACCGAATGTGA | ||
3. | Glial cell line derived neurotrophic factor family receptor alpha 1 (Gfra1) | gfra-115f | GAACAGAGCTGCAGCACCAA |
gfra-194r | CCGGATGTCAGGCTGAAGTT | ||
4. | Interferon induced transmembrane protein 3 (Ifitm3) | ifitm3-260f | GGAAGATGGTGGGTGATGTGA |
ifitm3-339r | GAGGACCAAGGTGCTGATGTTC | ||
5. | Integrin α6 (Itga6) | intα6-977f | ATGCAGATGGGTGGCAAGAC |
intα6-1056r | GTAAACTGCACCCCCGACTTC | ||
6. | Nanog | qnanog-771f | GCCTTACGTACAGTTGCAGCAA |
qnanog-850r | GCGCATGGCTTTCCCTAGT | ||
7. | POU domain, class 5, transcription factor 1(Pou5f1)/Oct3/4 | pou5f1-660f | GCAGGCCCGGAAGAGAAA |
pou5f1-739r | TCGGGCACTTCAGAAACATG | ||
8. | DEAD (Asp-Glu-Ala-Asp) box polypeptide 4(Ddx4) | vasa-1787f | CCAGCTTCAGTAGCAGCACAAG |
vasa-1866r | GTGTGCTTTGCCCTGGTAATTC | ||
9. | Dynein light chain 1(Dynlt1) | tctex-10f | TTCCAGGCCTCAGAGGAGACT |
tctex-89r | CCGATGGCGCTTTCTATAGC | ||
10. | Desmin (Des) | desmin-75f | ATGAGCCAGGCCTACTCGTC |
desmin-167r | CTCGAGGGAACACGGGAGAG | ||
11. | Alpha –fetoprotein (Afp) | AFP-1453 F | GAAGCAAGCCCTGTGAACTC |
AFP-1554R | GGCATAGGTTTCATCCCTCA | ||
12. | Nestin (Nes) | nestin-4986Â F | GGAAGAGAGTGGGGAAGAGG |
nestin-5101r | CATCCTGGACCTTGACACCT | ||
13 | cMyc | c-Myc F | CCTGTACCTCGTCCGATTCC |
c-Myc R | TTGTGTGTCCGCCTCTTGTC | ||
14 | Klf4 | Klf4 F | GCCCAACTACCCTCCTTTCC |
Klf4 R | CCATGATTGTAGCGCTTGCC | ||
15 | Lin28 | Lin28 F | GGGCTAGACCATCATGCCAA |
Lin28 R | ACTTGTTTCGCTTCCCGTCT | ||
16 | Nanog | Nanog F | AAATCCCTTCCCTCGCCATC |
Nanog R | ACCGCTTGCACTTCATCCTT | ||
17 | Oct4 | Oct4 F | TGGCTTCAGACTTCGCCTTC |
Oct4 R | GAAGCGACAGATGGTGGTCT | ||
18 | Sox2 | Sox2 F | ATGCACAACTCGGAGATCAG |
Sox2 R | GTTCATGTAGGTCTGCGAGC |