Fig. 1

scRNA-Seq of MSCs. The MSCs expanded with two batches of conventional MSC culture medium containing PL (human platelet lysate, PL1 and PL2) and full chemical defined medium (CDM1 and CDM2) were subject to single-cell RNA sequencing (scRNA-seq). A Cell cluster identification via non-linear dimensionality reduction analysis with UMAP after integrating 4 samples. B Subpopulation distribution and constitution in MSCs expanded with different media. C Percentage of different clusters in MSCs expanded with different media. D Correlation analysis of different MSC clusters. E Clustering analysis of different MSC clusters. F The more correlated clusters were labelled with colors