Fig. 2
Cy injection causes acute ovarian damage. A The establishment process of the POI rat model. B Representative images of estrous cycles. C The rates of estrous cycle disorder. D–G Serum levels of E2 (D), FSH (E), LH (F), AMH (G) (n = 6). H–I Ovarian weights and indexes (n = 6). Ovarian index (‰) = ovarian weight (mg)/rat weight (g) × 1,000‰. J–M The numbers of primordial (J), primary (K), secondary (L), and antral follicles (M) (n = 6). N The numbers of total follicles (n = 6). O The numbers of atresia follicles (n = 6). P The structure of ovaries. a Representative primordial follicles, b Representative secondary follicles, c Representative antral follicles, and d Representative atresia follicles. Bar: 100 μm.*P < 0.05, ** P < 0.01, *** P < 0.001; **** P < 0.0001