Primary Antibody | Host | Dilution | Manufacturer |
---|---|---|---|
Motor neuron differentiation | |||
SOX1 | Goat | 1:50 †| R&D SYSTEMS |
OLIG2 | Rabbit | 1:50 †| Millipore |
1:700 * | |||
ChAT | Goat | 1:50 †| Millipore |
1:700 * | |||
HB9 | Rabbit | 1:50 †| Millipore |
SMI-32 | Mouse | 1:50 †| BioLegend |
1:700 * | |||
Cell proliferation | |||
Ki-67 | Goat | 1:50 †| Santa cruz |
Neuron, Astrocyte, Reactive Astrocyte and Oligodendrocyte | |||
Neu N (Neuron) | Mouse | 1:100 †| Abcam |
1:700 * | |||
GFAP (Astrocyte) | Mouse | 1:100 †| Merck |
1:700 * | |||
Rabbit | 1:1000 †| Abcam | |
1:10000 * | |||
C3 (Reactive astrocyte, A1) | Rabbit | 1:500 †| Abcam |
1:1000 * | |||
CC-1 (Oligodendrocyte) | Mouse | 1:100 †| Merck |
1:700 * | |||
Growth factors | |||
NGF | Rabbit | 1:50 †| Abcam |
1:700 * | |||
BDNF | Rabbit | 1:50 †| GeneTex |
1:700 * | |||
VEGF | Mouse | 1:50 †| Santa cruz |
1:700 * | |||
Synaptogenesis and Axonal regeneration | |||
Synapsin-1 | Rabbit | 1:50 †| Sigma-Aldrich |
1:700 * | |||
MAP-2 | Mouse | 1:50 †| Santa cruz |
1:700 * | |||
House Keeping Protein | |||
GAPDH | Mouse | 1:2000 * | Abcam |
Beta actin | Rabbit | 1:2000 * | Abcam |