Fig. 2
From: Mesenchymal stem cells promote ovarian reconstruction in mice
Mouse MSCs benefit ovarian development in reconstituted ovaries. Reconstituted ovaries were obtained after 14 days of in vivo development. (A) Morphology of control-rOvaries and MSC-rOvaries at 14 days after transplantation. Scale bars, 1 mm. (B) HE staining was performed to observe the internal growth of the reconstituted ovaries, and it was seen that the follicular development in the MSC-rOvaries group was better than that of the control group. Scale bar,50 μm. (C) Distribution of follicles in r-Ovaries without and with MSCs. n = 3/per group. Primo, primordial follicle; Prima, primary follicle; Sec, secondary follicle; Ant, antral follicle. Scale bar, 50 μm. (D) Relative expression of follicular growth and development related gene in the rOvaries were assessed by real-time PCR. n = 3/per group. Data are presented as means ± SEM.*P < 0.05 and ***p < 0.001. (E) Immunohistochemistry analyses the expression of PCNA in reaggregated ovaries after transplanted 14 days. Scale bar, 50 μm. (F) Immunohistochemistry analyses expression of AMH in aggregated ovaries after transplanted 14 days. Scale bar, 50 μm.