Fig. 3

Evaluation of oocyte quality by IVM, IVF and early embryonic development. Aggregated ovaries with mouse MSCs were transplanted into the kidney capsules of recipient mice for 18 days. GV oocytes for IVM were collected by directly puncturing fully grown follicles under the microscope and mature MII oocytes were retrieved after a single injection of hCG into recipient mice after transplanted 24 days (IVO). (A) Morphology of MII oocytes after IVM. Oocytes from superovulated ovaries of 3-week-old mice were used as negative controls (Negative ctrl). And Immunofluorescence of β-tubulin on spindle of MII oocytes. Green, β-tubulin; Blue, Nuclear staining with Hoechst 33342. (B) Percentages of MII oocytes with aberrant spindles after IVM treatment. (C) Morphology of MII oocytes obtained from MSC-rOvaries after a single injection of hCG into recipient mice after transplanted 24 days (IVO). And Immunofluorescence of β-tubulin on spindle of MII oocytes. Green, β-tubulin; Blue, Nuclear staining with Hoechst 33342. (D)Representative 2-cell embryos and blastocysts after IVO oocytes being fertilized in vitro (IVF). (E) Efficiency of early embryonic development. Percentage of IVO mature oocytes in negative controls and MSC-rOvaries group capable of developing into 2-cell embryos and blastocysts. All experiments were performed with at least three replicates. Data were shown as mean ± SEM. *p < 0.05. Scale bar, 50 μm.