Fig. 4
From: Mesenchymal stem cells promote ovarian reconstruction in mice
Mouse mesenchymal stem cells reduce apoptosis in reconstituted ovaries. (A) TUNEL assay of reaggregated ovaries after in vitro culture. (B) The Control group of rOvaries cultured in vitro for 24 h, TUNEL co-stained with immunofluorescence of germ cell marker VASA antibody. The apoptotic signal was present in VASA-positive oocytes. (Scale bar: 50 μm.). (C) The statistical analysis of TUNEL was performed to detect apoptosis within the tissues of rOvaries at various time points in the early post-recombinational period. ***p < 0.001. n = 4/each group. GFP-labeled MSCs were used to track the growth of reaggregated ovaries. (D) GFP-MSCs in reaggregated ovaries after recombination. (E) Analysis of scanning intensity statistics of green fluorescent signals carried by mesenchymal stem cells in MSC-rOvaries. n = 5/each group. *p<0.05. (F) Immunofluorescence labeling of proliferation by PCNA antibody, PCNA red fluorescent signal did not co-localize with GFP. (G) Western blot showed the increased phosphorylation of Akt, mTOR and RPS6 in BM-MSCs reconstituted ovaries after 96 h of culture.Scale bar, 50 μm.