Fig. 3
From: DPSCs regulate epithelial-T cell interactions in oral submucous fibrosis
Histological analyses of the therapeutic effects of different treatments on arecoline-induced OSF rats. (A) Representative images of the oral mucosa of rats with different treatments (4 and 8 weeks post-injection). Control: normal rats, OSF: non-treated OSF rats, OSF + PBS: PBS injected OSF rats, OSF + GC: glucocorticoid/PBS injected OSF rats, OSF + DPSCs: dental pulp stem cells/PBS injected OSF rats. (B) Quantitative analysis of the lesion area (white) in (A). n = 6. (C) Representative images of hematoxylin and eosin (H&E) staining for epithelial morphology at 4 and 8 weeks. Scale bar = 500 μm. (D) Mouth opening measurements in rats after different treatments at specified intervals (0, 4, and 8 weeks post-injection), n = 6. (E) Representative Masson’s trichrome images of different treatments at 4 and 8 weeks. Scale bar = 500 μm. (F) Quantification of the amount of collagen deposition (blue) on the submucosa in Masson’s trichrome images at 4 and 8 weeks, n = 6. (G) Representative Sirius Red images of oral mucosal observed under polarized light after different treatments at 4 and 8 weeks. The white arrows indicate positive areas. Scale bar = 50 μm. (H) Quantification of type III collagen (green) areas on the submucosa in Sirius Red images at 4 and 8 weeks, n = 6. The results are presented as the mean ± S.D. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001