Figure 2
MSCs increase NO production in MSC/BV2 cocultures. MSCs and BV2 cells were seeded simultaneously into 24-well plates at ratios indicated below the graph. After overnight incubation, 1 μg/ml LPS was added to cocultures, and NO2- concentration was determined with the Griess assay at 18, 24, 48, 72, and 96 hours. Values are expressed as mean ± SD of triplicate wells and from a representative of two independent experiments. Control is BV2 cells treated with LPS. *P < 0.05 compared with control for direct cocultures and compared with direct coculture for transwell cocultures. LPS, lipopolysaccharide; MSC, mesenchymal stem cell; NO, nitric oxide; NO2-, nitrite; SD, standard deviation.