Figure 6

Dil tracing cells observed under fluorescence microscopy and immunohistochemistry. The DiI tracing SP cells emerged near vessels and significantly formed part of one vessel in an amputated nude mouse limb (A) but there was no signal in the PBS-treated limb (B). The positive mouse anti-human CD54 can be found in corresponding DiI positive cells, which indicates that the vessel was composed of differentiated SP cells (C); the control limb had no positive signal or staining (D). PBS, phosphate-buffered saline; SP, side population.