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Figure 5 | Stem Cell Research & Therapy

Figure 5

From: Human amniotic fluid-derived and dental pulp-derived stem cells seeded into collagen scaffold repair critical-size bone defects promoting vascularization

Figure 5

Presence of human stem cells in vessels 8 weeks after surgery. H & E staining of cranial defects (A) closed with collagen + dental pulp stem cells (DPSC) and (B) closed with collagen + amniotic fluid stem cells (AFSC). Scale bar = 100 μM. Diaminobenzidine staining of cranial defects (C) closed with collagen + DPSC and (D) closed with collagen + AFSC. Scale bar = 100 μM. Confocal images of implants obtained 8 weeks after surgery. Double fluorescence signals from 4’,6-diamidino-2-phenylindole (DAPI; red) and anti-human mitochondria protein (green) antibody images: (E) collagen colonized with DPSC, (F) collagen colonized with AFSC. Triple fluorescence signals from DAPI (blue) and anti-human mitochondria protein (red) antibody images or DAPI (blue) and anti-von Willebrand (green) antibody images: (G), (I) collagen colonized with DPSC, (H), (K) collagen colonized with AFSC. Scale bar = 50 μM.

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Stem Cell Research & Therapy

ISSN: 1757-6512