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Figure 2 | Stem Cell Research & Therapy

Figure 2

From: Mesenchymal stem cells and their chondrogenic differentiated and dedifferentiated progeny express chemokine receptor CCR9 and chemotactically migrate toward CCL25 or serum

Figure 2

Isolation of chondrogenically differentiated cells and their confirmation. Chondrogenically differentiated cells were isolated from chondrogenic pellets. (A) Their chondrogenic character showed a positive collagen type II, (C) and Alcian blue staining, whereas the controls were negative for both (B) collagen type II (D) and Alcian blue staining. (E) After 28 days of induction, the chondrogenically differentiated cells showed significantly upregulated expression for cartilage-specific genes like COL2A1 and SOX9 compared with controls and undifferentiated cells, day 0 (n = 3), mean ± SEM. (F) Flow-cytometric analysis showed a relatively lower expression of CD44, CD73, CD90, CD105, and CD166 antigens compared with undifferentiated MSCs (n = 3). Student t test was performed for statistical analysis, and asterisks were assigned in the order P* < 0.05, P** < 0.01, and P*** < 0.001, mean ± SEM. Bar, 200 μm.

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Stem Cell Research & Therapy

ISSN: 1757-6512