Figure 6From: Mesenchymal stem cells and their chondrogenic differentiated and dedifferentiated progeny express chemokine receptor CCR9 and chemotactically migrate toward CCL25 or serumAnalysis of CCR9, a cellular receptor of CCL25 chemokine. The immunohistochemical analysis of CCR9 showed greater staining for (A) the undifferentiated state compared with (D) chondrogenic differentiated and (G) dedifferentiated states of MSCs. The percentage flow-cytometric measurement for extracellular and intracellular domains of CCR9 is given in the form of average (n = 3) with a horizontal line, and bars represents a minimum or maximum expression for (C) undifferentiated state, (F) chondrogenic differentiated, and (I) dedifferentiated states of MSCs. (J) On the gene level, the qPCR analysis revealed significantly upregulated expression for CCR9 receptor in the chondrogenic differentiated state compared with undifferentiated and dedifferentiated states of MSCs (n = 3). Student t test was performed for statistical analysis, and asterisks were assigned in the order P* < 0.05, P** < 0.01, and P*** < 0.001, average ± SD. Bar, 200 μ m.Back to article page