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Figure 5 | Stem Cell Research & Therapy

Figure 5

From: Assessment of immunosuppressive activity of human mesenchymal stem cells using murine antigen specific CD4 and CD8 T cells in vitro

Figure 5

hMSCs protect CD8+T cells from apoptosis. Negatively selected CD4 and CD8 murine T cells were co-cultured with hMSC in the presence of specific peptide and APCs for three days. Cells were stained with annexin V - APC and analyzed immediately on a FACS Canto II BD instrument. Results were analyzed using (Tree Star, Inc., Ashland, OR) software. The experimental conditions are shown to the left of each set of panels. The histograms are representative of three independent experiments with similar results. The horizontal bar shows the gate used to determine the percentage of apoptotic cells after treatment. hMSCs, human bone marrow-derived mesenchymal stem cells.

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Stem Cell Research & Therapy

ISSN: 1757-6512