Figure 6

hMSCs inhibit expression of T-bet and GATA-3 in murine CD4⁺and CD8⁺T cells. Total RNA was extracted from murine T cells 48 hrs after stimulation with specific peptides and co-culturing with human bone marrow-derived mesenchymal stem cells (hMSCs). The RNA was reverse transcribed into cDNA and specifically amplified using probes for murine T-bet and GATA-3. Bars in each panel represent fold change of mRNA expression as compared with un-stimulated T cells. Results for CD4⁺ T cells are shown in panel A and for CD8⁺ T cells in panel B. Every assay was done in triplicate, values are expressed as the mean and SD of triplicates. Statistical analysis was carried out by applying the Student’s t- test. *indicates P <0.05 for differences between conditions shown by the horizontal brackets. The results shown represent one out of three independent experiments with similar results.