| Passage 5 | Passage 45 |
---|
CD45 | 20.5 | 1.7 |
SCA1 | 26.9 | 30.1 |
SSEA4 | 10.9 | 55.4 |
MHC Class I | 2.9 | 1.8 |
MHC Class II | 10.8 | 1.5 |
- The number of cells positively expressing SCA1 remained relatively stable as passage number increased. A decrease in the hematopoietic stem cell marker CD45 was observed as cell passage increased. Also, an increase of expression of the stem cell marker SSEA4 between low- and high-passaged cells occurred. Expression of MHC Class I remained relatively stable between low- and high-passaged cells, but a decrease in MHC Class II was observed between low- and high-passaged cells, respectively. MHC, major histological complex; SSEA4, stage specific embryonic antigen 4.