Table 1 Oligonucleotide sequences used for RT-PCR analysis

Housekeeping gene

GAPDH

Forward, 5′ – ACGATGACATCAAGAAGGTG – 3′

180 bp

Reverse, 5′ – CATACCAGGAAATGAGCTTG – 3′

Pluripotent markers

Oct4

Forward, 5′ – GGAGTCCCAGGACATCAAAG – 3′

285 bp

Reverse, 5′ – GCCTGCACAAGTGTCTCTGC – 3′

Nanog

Forward, 5′ – ACGGATCCAGCTCAGCCCCA – 3′

192 bp

Reverse, 5′ – GGGGCTGCCCTGAGCAAGTA – 3′

Mesenchymal markers

CD44

Forward, 5′ – TGGGTTGTTTGGCATCCAGTGC – 3′

100 bp

Reverse, 5′ – CGTTTTCTTCAGTTGGTTCCCAGCC – 3′

CD166

Forward, 5′ – ACTGGCAGTGGAAGCGTCAT – 3′

275 bp

Reverse, 5′ – CAGCAAGGAGGAGACCA – 3′

CD29

Forward, 5′ – GGAAACTTGGTGGCATTGTT – 3′

180 bp

Reverse, 5′ – GTTCCTTGTAAACGGGCTGA – 3′

CD73

Forward, 5′ – AGCAAAGGGGCCACTAGCATCT – 3′

233 bp

Reverse, 5′ – ACCCGAATGTCCCAGTGCAA – 3′

CD90

Forward, 5′ – GAGCACACGTACCGCTCCCG – 3′

233 bp

Reverse, 5′ – AGCAGCAGCAGCAGCATCCA – 3′

Hematopoietic marker

CD34

Forward, 5′ – CTTTAACTGTCACGGCGTTT – 3′

198 bp

Reverse, 5′ – TGACTCGGGAACATTTGATT – 3′

Immunological markers

MHC-I

Forward, 5′ – CATCACCCTGAGATGGGAGC – 3′

176 bp

Reverse, 5′ – TGGGTACTGTCGTCGCGTG – 3′

MHC-II

Forward, 5′ – TCCGGAATCAGAAAGGACAC – 3′

172 bp

Reverse, 5′ – GGCAAACCAAATCCTGAGAA – 3′

Osteogenic markers

OCN

Forward, 5′ – CTGCCTCTGCCTGGCTGGTC – 3′

120 bp

Reverse,5′ – TAGCGCCGGAGCCTCCTCAC – 3′

OPN

Forward, 5′ – ACTGGTCACTGATTTTCCCACGGA – 3′

100 bp

Reverse, 5′– AACCACACTATCACCTCGGCCA – 3′

Adipogenic markers

ADPQ

Forward, 5′ – TGAGAAAGGAGATCCAGGTC – 3′

308 bp

Reverse, 5′ – TCAAGTAGACTGTGATGTGG – 3′

PPAR-γ

Forward, 5′ – CATGGTTGACACAGAGATGC – 3′

239 bp

Reverse, 5′ – GCTCCACTTTGATTGCACTTTG –3′

Chondrogenic markers

ACAN

Forward, 5′ – AAGTGGAGCCGCGTTTCCAAGG – 3′

163 bp

Reverse, 5′ – AGTCATTGGAGCGCAGGTTCTGG – 3′

COL2A1

Forward, 5′ – AGTTGGGAGTAATGCAAG – 3′

294 bp

Reverse, 5′ – GATAACCTCTGTGACCTTTG – 3′

Neurogenic marker

NES

Forward, 5′ – AAACAGGGCCTACAGAG – 3′

293 bp

Reverse, 5′ – ACAGGTGTCTCAAGGGTAG – 3′