Figure 6From: Von willebrand factor increases endothelial cell adhesiveness for human mesenchymal stem cells by activating p38 mitogen-activated protein kinaseTreatment of HUVECs with neutralizing antibodies against E-selectin, P-selectin, VCAM1 and ICAM1. Endothelial cells were treated with 4 μg/ml vWF in HBSS for four hours and exposed to 10 μg/ml matching isotype-control (IgG) or 10 μg/ml neutralizing antibodies against E-selectin, P-selectin, VCAM1 or ICAM1 for 40 minutes before hMSC adhesion assay. Data are shown as mean ± SD of eight independent measurements. Isotype-matching control and neutralizing antibodies against E-selectin, P-selectin, VCAM1 or ICAMP1 had no significant affect on hMSC adhesion to HUVECs treated with vWF (t-test, P-value >0.05).Back to article page