Figure 1
From: Toward a 3D model of human brain development for studying gene/environment interactions
Generation of neurons from induced pluripotent stem cells. (a) Phase contrast images showing undifferentiated induced pluripotent stem cells (iPSCs; left panel); neural progenitor cells (NPCs) 7 days after neural induction (middle panel) and after passage 1 of the NPCs (right panel). (b) Expression of NPC markers Sox-2 and nestin co-stained with 4',6-diamidino-2-phenylindole (DAPI; left and middle panels) show maintenance of undifferentiated NPCs over several passages. Cells expressing neural markers (microtubule-associated protein-2; MAP2) and synaptic (synapsin1), co-stained with DAPI, 21 days after differentiation (right panel). (c) Expression of neuronal marker (MAP2) plus co-expression of markers for specific cortical layers (Tbr1, Brn2, and Ctip2), co-stained with DAPI, 4 weeks after differentiation.