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Table 2 Quantitative real-time PCR analysis of expanded human MSCs

From: A novel serum-free medium for the expansion of human mesenchymal stem cells

qRT-PCR

SFM

SCM

Target

Expression

ΔCt

Expression level

ΔCt

Positive Human MSC markers

CD44

N/A

N/A

N/A

N/A

CD73

++++

2.55

++++

3.53

CD90

++++

2.40

++++

2.63

CD105

++++

2.63

++++

1.82

Nestin

+++

5.79

++

11.17

  1. Quantitative real-time PCR analysis of known positive human MSC surface antigens for cells expanded in both SFM and SCM after four passages. ΔCt, Ct (gene-of-interest) - Ct (GAPDH), ++++, very high; +++, high; ++, moderate; +, low.
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Stem Cell Research & Therapy

ISSN: 1757-6512