Nuclear reprogramming with a non-integrating human RNA virus
© Driscoll et al.; licensee BioMed Central. 2015
Received: 4 September 2014
Accepted: 3 March 2015
Published: 26 March 2015
Advances in the field of stem cells have led to novel avenues for generating induced pluripotent stem cells (iPSCs) from differentiated somatic cells. iPSCs are typically obtained by the introduction of four factors—OCT4, SOX2, KLF4, and cMYC—via integrating vectors. Here, we report the feasibility of a novel reprogramming process based on vectors derived from the non-integrating vaccine strain of measles virus (MV).
We produced a one-cycle MV vector by substituting the viral attachment protein gene with the green fluorescent protein (GFP) gene. This vector was further engineered to encode for OCT4 in an additional transcription unit.
After verification of OCT4 expression, we assessed the ability of iPSC reprogramming. The reprogramming vector cocktail with the OCT4-expressing MV vector and SOX2-, KLF4-, and cMYC-expressing lentiviral vectors efficiently transduced human skin fibroblasts and formed iPSC colonies. Reverse transcription-polymerase chain reaction and immunostaining confirmed induction of endogenous pluripotency-associated marker genes, such as SSEA-4, TRA-1-60, and Nanog. Pluripotency of derived clones was confirmed by spontaneous differentiation into three germ layers, teratoma formation, and guided differentiation into beating cardiomyocytes.
MV vectors can induce efficient nuclear reprogramming. Given the excellent safety record of MV vaccines and the translational capabilities recently developed to produce MV-based vectors now used for cancer clinical trials, our MV vector system provides an RNA-based, non-integrating gene transfer platform for nuclear reprogramming that is amenable for immediate clinical translation.
Human pluripotent stem cells may replace non-functional tissues because of their unique capability of giving rise to any cell type in the body. Advances in stem cell research resulted in several processes to generate induced pluripotent stem cells (iPSCs) from adult somatic cells. These iPSCs are typically obtained by the introduction of three to four factors such as OCT4, SOX2, KLF4, and cMYC, which are highly expressed in embryonic stem cells. Derived iPSCs demonstrate properties that are very similar to those of embryonic stem cells [1-3]. Generation of iPSCs from the patient’s own tissues allows novel autologous stem cell therapies while circumventing immunological mismatch and ethical issues associated with the use of embryonic cell sources.
Several vectors have been developed to deliver pluripotency-associated genes or proteins for cellular reprogramming, including integrating lentiviral (LV) and retroviral vectors, RNAs, proteins, and plasmids [4-10]. All of these carriers were used to reprogram fibroblasts into iPSCs. However, efficient reprogramming of patient-derived somatic cells is still challenging. In addition, the use of integrating vectors adds concerns over tumorigenicity because of insertional mutagenesis.
Recently, Sendai virus (SeV), a murine Paramyxovirus with a mutant fusion (F) glycoprotein, was developed as an efficient RNA-based gene delivery vector [11,12]. This vector allows a robust and sustained expression of foreign genes. SeV is an enveloped virus with a non-segmented negative-strand RNA genome . Its life cycle/RNA replication occurs in the cytoplasm without DNA intermediates, minimizing the risk of vector genome (RNA) integration into the host genome [14,15]. Efficient, integration-free iPSC derivation from patients with type 1 diabetes was described documenting the successful use of this negative-strand RNA virus for nuclear reprogramming [16,17]. While SeV is in a phase I cancer clinical trial , its safety credentials in humans are limited. This may become a critical barrier for rapid translation into future iPSC clinical studies.
Measles virus (MV) is a human Paramyxovirus, and its vaccine strain has a well-established safety record in nearly a billion children . Like SeV, MV has a non-segmented negative-strand RNA genome, which is transcribed and replicated in the cytoplasm without production of DNA intermediates [13,20-22]. The MV genome contains six genes—N, P, M, F, H, and L—that encode eight proteins: the nucleoprotein (N), the phosphoprotein (P), the matrix protein (M), the two glycoproteins hemagglutinin (H) and fusion protein (F), the viral polymerase (L), and the two accessory proteins V and C .
Using reverse genetics, we have generated genomic cDNAs with the coding capacity identical to that of an MV Moraten/Schwartz vaccine strain. These genomic cDNAs have regularly spaced unique restriction sites to assist in mutagenesis and additional transcription unit (ATU) inserted in different positions, allowing expression of foreign genes [23-25]. As MV transcription is gradually reduced the farther the polymerase travels down the genome, the different location of these ATUs along the genome allows differential levels of expression of the transgene. These vectors, which have been used to express antigens from different pathogens [23,26-32], can accommodate up to 6 kilobases of foreign genetic material, allowing insertion of several genes. MV was also developed as an oncolytic vector [33,34]. Four phase I or II clinical trials are currently active, and no serious adverse events have been reported [33,35,36].
We present here the first application of MV as a vector for nuclear reprogramming. We have produced a one-cycle MV vector expressing one of the four reprogramming factors: OCT4. We conducted a proof-of-principle experiment by substituting LV expressing OCT4 with an MV-based vector expressing OCT4 in a four-vector reprogramming system. We reprogrammed newborn somatic cells into iPSCs and characterized these MV-derived iPSCs for expression of stem cell markers, induction of endogenous pluripotency-associated genes, and pluripotency.
All animal studies were approved by the Mayo Institutional Animal Care and Use Committee. The human cell line used in this study was purchased from the American Type Culture Collection (Manassas, VA, USA) and did not require consent or the need for Mayo Institutional Review Board approval.
Cells and viruses
Vero-H2 cells and the helper 293-3-46-H2 cell line were produced by transducing Vero cells (ATCC #CCL81; American Type Culture Collection) and the helper 293-3-46 cell line  with an LV encoding for the MV hemagglutinin gene. Vero-H2, Vero, and 293T cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Mediatech Inc., Herndon, VA, USA) supplemented with 10% fetal calf serum (FCS), 1% penicillin/streptomycin (Mediatech) (DMEM-10), and helper 293-3-46-H2 cells in DMEM-10 containing 1.2 mg/mL G418 (Mediatech). The human BJ cells, neonatal foreskin human cells, were purchased from the American Type Culture Collection (ATCC #CRL 2522). BJ cells were maintained in DMEM-10 containing 0.1 mM of non-essential amino acids. iPSCs were maintained in 80% Pluriton (Stemgent, Cambridge, MA, USA) and 20% mTeSR1 (Stemcell Technology, Vancouver, BC, Canada) mixed medium supplemented with 1% penicillin/streptomycin.
Recombinant MVs were generated as described by Radecke et al. . Briefly, the helper 293-3-46-H2 cell line was transfected by calcium phosphate precipitation by using a ProFection kit (Promega, Madison, WI, USA) with two plasmids, one coding for the relevant MV genome and the other for the MV polymerase (pEMCLa). Three days after transfection, the helper cells were overlaid on Vero-H2 cells, the appearance of infectious centers was monitored, and single viruses were propagated on Vero-H2 cells. To prepare virus stocks, Vero-H2 cells were infected at a multiplicity of infection (MOI) of 0.03 and incubated at 32°C for 4 to 5 days or until 95% of the cells expressed green fluorescent protein (GFP). Cells were scraped in Opti-MEM (Life Technologies, Grand Island, NY, USA), and particles were released by two freeze-thaw cycles. Virus stock titers were determined by 50% end-point dilution (tissue culture infectious dose 50, or TCID50) on Vero-H2 cells by using the Spearman-Kärber method .
Expression plasmids, full-length measles virus infectious cDNA, and lentiviral vector vectors
To produce the p(+)MVvac2(OCT4)P full-length cDNA vector, the open reading frame of OCT4 was inserted into the ATU located between P and M genes in the full-length cDNA of pB(+)MVvac2(ATU) [23,24]. Cloning was performed by using the following primers: 5′-acgcgtcgtacgtcgcgaatgtacaacatgatggagac-3′ and 5′-gcgcgctacgtatcgcgatcacatgtgtgagaggg-3′ and the In-Fusion HD Cloning kit (Promega). To generate one-cycle virus, the H gene was substituted with the GFP gene. The GFP open reading frame was amplified by using the following primers: 5′-cggtagttaattaaaacttagggtgcaagatcatcgatatcgcgaatggtgagcaagggcg-3′ and 5′-gacgagctgtacaagtagttcgcgacccgggaagatggaaccaat-3′. The substitution was performed in the pCG-Hvac2 plasmid by using the PacI and SmaI sites. To obtain the full-length p(+)MVvac2(OCT4)P-ΔH(GFP), cDNA was then obtained by transfer of a PacI-SpeI fragment in the p(+)MVvac2 . The LV encoding the MV H (LV-H) was produced by cloning the open reading frame of H into the pSIN LV by using the MluI and NotI restriction sites.
LV particles generated from the plasmids pSIN-H, pSIN-GFP, pSIN-OCT4, pSIN-SOX2, pSIN-KLF4, and pSIN-cMYC were produced as described previously . Briefly, the cDNA vectors were co-transfected into 293 T cells along with a packaging plasmid (pCMVR8.91) and a plasmid encoding VSV-G (vesicular stomatitis virus G protein) for pseudotyping (pMD-G)  by using the Fugene 6 kit (Life Technologies) in accordance with the instructions of the manufacturer. Two days after transfection, the supernatant was centrifuged at 3,000 revolutions per minute for 15 minutes, filtered on 0.45-μm filters, and aliquoted and stored at −80°C. An LV-GFP stock was always produced in parallel with any LV productions and used for semi-quantitative viral titration. LV titration was performed by transduction of BJ cells by using 25, 50, 75, 100, 150, and 200 μL of viral stock. The lowest volume (on average, 50 μL) of viral stock determined to give 90% to 100% of green (GFP) fluorescent cells was then used in future transduction experiments. The Mayo Clinic Institutional Biosafety Committee has approved all vectors, viruses, and experiments performed in this study.
H surface expression and infection efficiency quantification by flow cytometry
Vero, Vero-H2, 293-3-46, and 293-3-46-H2 cells were incubated with rabbit anti-H diluted in phosphate-buffered saline (PBS)-4% FCS  for 1 hour at 4°C. After two washes, the cells were incubated with a fluorescein isothiocyanate (FITC)-labeled anti-rabbit secondary antibody diluted in PBS-4% FCS (Jackson ImmunoResearch Laboratories, West Grove, PA, USA) for 1 hour at 4°C. The cells were washed twice and fixed in PBS-2% paraformaldehyde (PFA) (Affymetrix, Inc., Cleveland, OH, USA). Flow cytometry data were acquired on a Becton Dickinson FACS Calibur (Becton Dickinson, Franklin Lakes, NJ, USA) and analyzed by using Flowjo software.
BJ cells were transduced or not with MV(OCT4) vector at an MOI of 6 for 48 hours. The cells were washed once with PBS and detached with Versene. After two washes with PBS, the cells were fixed with PBS-2% PFA. GFP-expressing cells were analyzed by flow cytometry as described above.
Measles virus vector infectivity
Vero and Vero-H2 cells were infected with MV(OCT4) or MV control virus at an MOI of 0.05 or 0.1 for 2 hours at 37°C in Opti-MEM. After 2 hours, the inoculum was removed, cells were washed once, and 1 mL of medium was added to the cells. Cells were then incubated at 37°C. Cells were collected at two different time points: 24 and 48 hours post-infection. Cells were collected in the 1 mL of supernatant and freeze-thawed once to release cell-associated virus. Viral titers were determined by 50% end-point dilution (tissue culture infectious dose 50, TCID50) on Vero-H2 cells by using the Spearman-Kärber method .
Reprogramming of human foreskin fibroblasts
Reprogramming of BJ foreskin cells (5 × 104) was performed by transduction with three LVs expressing SOX2, KLF4, or cMYC and one MV vector expressing OCT4. Control reprogramming was performed by using either four LVs or three LVs only (without OCT4). The amount of LV required for the most efficient reprogramming was tested by transducing the cells with different amounts of the four LVs, starting with the lowest dose of virus determined to give 90% to 100% of GFP-positive cells. The amount of LV showing the highest efficiency to produce iPSC colonies was used to reprogram with the MV(OCT4) vector. Six days after transduction, cells were detached with Versene and replated on Matrigel-coated plates (BD Biosciences, San Jose, CA, USA) and fed with the serum-free human iPSC media consisting of Pluriton medium (Stemgent) supplemented with 20% (vol/vol) mTeSR-1 maintenance media (Stemcell Technologies). One to three weeks after vector infection, reprogrammed cells began to form iPSC-like colonies, and at 3 to 6 weeks, colonies were picked on the basis of size and morphology.
Immunoblot analysis of cell extracts
BJ and Vero cells (5 × 104) were transduced with LV(OCT4) or MV(OCT4) vectors by using the same conditions used for the reprogramming transduction. After 36 hours, the cells were processed as described elsewhere . After fractionation on 4% to 15% SDS-polyacrylamide gels (Bio-Rad Laboratories, Hercules, CA, USA) and transfer to polyvinylidene difluoride membranes (Immobilon-P; Millipore, Billerica, MA, USA), the samples were subjected to enhanced chemiluminescence detection by using the antibodies indicated. The following antibodies were used: rabbit anti-OCT4 (Cell Signaling Technology, Inc., Danvers, MA, USA) and anti-β-actin (Sigma-Aldrich, St. Louis, MO, USA) as a loading control.
Immunostaining and confocal microscopy
Cells (2.5 × 104 Vero or BJ) were plated in chamber slides (Lab Tek II Chamber Slide system; Nalge Nunc International Corp., Naperville, IL, USA) and were transduced with either the LV(OCT4) or MV(OCT4) by using the same conditions used for the reprogramming transduction. Thirty hours after transduction, cells were washed once with PBS and fixed with PBS-4% PFA for 15 minutes. Cells were then permeabilized with PBS-4% PFA-0.1% Triton X-100 for 15 minutes, washed with PBS, incubated for 1 hour in blocking solution (PBS-4% FCS), and immunostained by using a rabbit antibody directed against the human OCT4 (#2750; Cell Signaling Technology). Incubations were performed overnight at 4°C in PBS-2% FCS. After five washes, the cells were incubated for 1 hour with a Rhodamine anti-Rabbit secondary antibody (Jackson ImmunoResearch Laboratories). After the five washes, cells were mounted with Prolong Gold Antifade reagent containing DAPI (4′,6-diamidino-2-phenylindole) (Life Technologies) and analyzed with a Zeiss LSM 780 confocal microscope (Zeiss, Oberkochen, Germany). Images were analyzed by using the Zen black software from Zeiss.
For the characterization of undifferentiated iPSC clones, iPSCs were plated in Matrigel-coated chamber slides, fixed, and permeabilized as described above. After blocking, the cells were incubated overnight with the following antibodies: anti-MV-N (Cl25 ), SSEA-1, SSEA-4, TRA-1-60, TRA-1-81 (#SCR001; Millipore), OCT4 (#2750; Cell Signaling Technology), SOX2 (#2748; Cell Signaling Technology), and NANOG (#ab21624; Abcam, Cambridge, MA, USA). After three washes, secondary antibodies AlexaFluor 488 anti-mouse and AlexaFluor 488 anti-rabbit (Life Technologies) were incubated for 1 hour at room temperature. After three washes, the cells were mounted and analyzed as described above. Finally, the alkaline phosphate staining was performed with an Alkaline Phosphatase Detection Kit (#SCR001; Millipore).
Cellular and viral gene transcription
Total RNA from one well of a six-well plate of iPSCs was isolated with the Trizol reagent (Life Technologies) and used for semi-quantitative RT-PCR analysis. Reverse transcription was performed by using the RNA to cDNA EcoDry TM Premix (Oligo dT) kit (Clontech, Mountain View, CA, USA). Platinum Taq DNA polymerase (Life Technologies) was used for PCRs. Reactions were performed in accordance with the manufacturer protocols. Primers used to measure transcription levels of pluripotency markers (OCT4, SOX2, KLF4, NANOG, GDF3, hTERT, and cMYC) were previously described . Primers used to measure transcription levels of the N mRNA in iPSC clones were 5′-cataaggctgttagagg-3′ and 5′-cccagaatcatgttgaat-3′.
Flow cytometry of pluripotency markers
MV- and LV-iPSC-derived cells were dissociated into single cells by using Versene. Extracellular staining was performed by incubating the cells (2 × 105) with the primary antibody TRA-1-60, TRA-1-81, SSEA-1, and SSEA-4 for 1 hour on ice in PBS supplemented with 2% FCS and 0.05% NaN3. After two washes with PBS with 2% FCS and 0.05% NaN3, the cells were incubated with an FITC-labelled anti-mouse secondary antibody (Jackson Research Laboratories) for 1 hour. Intracellular OCT4 antibody staining was performed by fixing 4 × 105 cells with PBS-2% PFA for 20 minutes. The cells were washed with PBS-2% FCS-0.05% NaN3 once and then permeablised for 20 minutes with 500 μL of saponin permeabilization buffer (SPB) (PBS supplemented with 1 mg/mL Saponin and 1% bovine serum albumin) at room temperature. The primary and secondary antibodies were incubated for 1 hour each in the SPB on ice. Finally, the cells were fixed in 2% PFA. Flow cytometry data were acquired on a Becton Dickinson FACS Calibur (Becton Dickinson) and analyzed by using Flowjo software.
Conventional cytogenetic analysis was performed on 20 metaphase cells of the iPSC clones. Chromosomes were banded in accordance with standard methods for high-resolution G-banding. Cells were captured and karyotyped by using a CytoVision Karyotyping System (Genetix, New Milton, UK).
Spontaneous differentiation assay
For spontaneous differentiation, MV- and LV-derived iPSC clones were dissociated by using Versene and cultured on low-adhesion plates in iPSC medium for embryoid body (EB) formation. EBs were cultured in suspension for 10 days and then allowed to adhere to a Matrigel-coated plate in DMEM with 20% fetal bovine serum and further cultured for 10 to 14 days. Differentiated cells were analyzed for markers of three germ layers. FOXA2 for endoderm (#07-633; Millipore), beta III tubulin (#ab41489; Abcam) for ectoderm, and CD31 (#sc-1506; Santa Cruz Biotechnology, Dallas, TX, USA) for mesoderm were used to stain the EB-derived cells.
Teratoma formation and in vivo differentiation of induced pluripotent stem cells
All studies were approved by the Mayo Clinic Institutional Animal Care and Use Committee. Female severe combined immunodeficiency (SCID)-beige mice (Charles River Laboratories, Wilmington, MA, USA) were anesthetized, and the kidney was exposed for iPSC transplantation under the kidney capsule. To this end, a small incision was made in the kidney capsule, and a blunt needle was used to create a pocket under the kidney capsule. After iPSC injection, the kidney was placed back into the abdomen, and the incision was closed. Mice were maintained for 4 weeks and sacrificed before harvesting of iPSC-transplanted kidneys. Tissues were embedded and flash-frozen in O.C.T Compound (Sakura Finetek USA, Inc., Torrance, CA, USA). Cryosections (7-μm) were obtained and immediately fixed in 4% PFA for 30 minutes at room temperature before proceeding to hematoxylin-and-eosin staining.
Differentiation of induced pluripotent stem cells into cardiomyocyte-like cells
iPSCs were plated in Matrigel-coated chamber slides. When the cells reached confluency, cardiac differentiation was started by using a PSdif-Cardio cardiomyocyte differentiation kit (StemRD, Burlingame, CA, USA). After 10 days of culture, the first contracting cells were observed under light microscopy. Video of the contracting cardiomyocyte-like cells was obtained by using a Zeiss Axiovert 200 M microscope and the ApoTome imaging system. The software used for the movie analysis is Axiovision from Zeiss. Immunostaining for α-actinin (#A7811; Sigma-Aldrich), a marker of microfilaments, and troponin (#Ab8295; Abcam) marker specific for the cardiac muscle was analyzed with a Zeiss LSM 780 confocal microscope.
Production of a ‘one cycle’ measles virus vector expressing the reprogramming factor OCT4
We analyzed the expression of OCT4 by immunoblot and immunofluorescence and compared it with the OCT4 expression after LV transduction (Figure 1C, D). After infection with MV(OCT4), both Vero and BJ cells showed a stronger expression of OCT4 compared with the control cells transduced with the LV(OCT4) (Figure 1C, compare first and second lines in each cell line). Although the higher infection efficiency of MV(OCT4) in Vero cells could partly account for the higher amount of OCT4 expression (Figure 1D, Vero, compare red panels, left half), in BJ cells infection levels were equivalent (Figure 1D, compare red panels, right half). Only single infected cells were detected, indicating that our vector does not fuse cells that do not express MV-H, as expected (Figure 1D, center panels, top row). To confirm the production of a ‘one cycle’ MV vector, we performed a one-step infection on cells expressing (Vero-H2) or not expressing (Vero) the MV-H protein. Cells were infected at an MOI of 0.05 or 0.1, and viral titers were determined 24 or 48 hours after infection. Figure 1E presents the viral titers of Vero infected with MV(OCT4) and control MV. Whereas a high viral titer is observed with the control MV, no virus was produced with the MV(OCT4). In contrast, the expression of MV-H at the cell surface restores efficient virus production (Figure 1F). These results confirmed that MV(OCT4) is a ‘one cycle’ vector and that expression of MV-H in trans is required for viral spread.
Reprogramming using MV(OCT4) instead of LV(OCT4) leads to production of induced pluripotent stem cell-like colonies
To measure the longevity of MV vector expression in the iPSC-like clones, we performed reverse transcription-polymerase chain reaction (RT-PCR) to detect the mRNA from the nucleoprotein (N) gene, which is the highest expressed gene during MV transcription. The three clones were propagated for over 18 passages and RNA was collected at passages 6, 8, 10, 12, 15, and 18 (Figure 2C). Only clone #2 showed some residual expression at passages 6 and 8 (53 to 59 and 61 to 67 days post-transduction, respectively), suggesting that the MV vector is eventually eliminated from the cells. Immunofluorescence analysis confirmed the absence of N expression at passage 6 in all three clones (Figure 2D).
Measles virus-derived induced pluripotent stem cell-like clones express pluripotency markers
Karyotyping analysis of derived iPSC clones was performed at passage 17. Most of the cells from clones #1 and #3 have normal diploid karyotypes (Figure 3D and Additional file 3: Figure S3). Only a small number of cells showed an abnormal karyotype with trisomy 12 or 14 (Additional file 3: Figure S3). Since frequent trisomy has been noted in human iPSCs and embryonic stem cells after prolonged passages [44-46], we conclude that the observed chromosomal abnormalities in these clones are due to continuous in vitro passage. Clone #2 showed abnormalities of 20q chromosome and such abnormalities have also been observed in prolonged culture of iPSCs [44-47]. Clone #2 also showed a loss of the Y chromosome. We speculate that this chromosome deficiency was present in the pre-reprogrammed parental fibroblast cell and was not induced during or after the reprogramming process (Additional file 3: Figure S3).
Measles virus-derived induced pluripotent stem cell clones can differentiate into the three germ lineages or into cardiomyocyte-like cells
In this study, we established the feasibility of using a non-integrating, non-pathogenic human negative-strand RNA virus as a vector to express factors required for the reprogramming of human cells into iPSCs. We substituted LV-based vector expressing OCT4 with an MV-based vector expressing this factor and produced iPSC clones. These clones have the same characteristics as those obtained with 4 LVs. Our results indicate that MV replication did not affect the process of reprogramming and that OCT4 was produced in sufficient quantity. Thus, MV can be used as a vector for efficient nuclear reprogramming.
Negative-strand RNA viruses as reprogramming tools
Among the main advantages of non-integrating negative-strand RNA viruses over other systems are that the entire viral cycle occurs in the cytoplasm and that there are no DNA intermediates . No DNA integration events were ever reported for the RNA genome of these viruses because of these two characteristics. Thus, there is no insertional mutagenesis risk into the genome of the iPSCs. It is possible to insert large genes in the MV genome because particle size is not constrained by icosahedral symmetry . Here, we showed for the first time that the vaccine strain of the human negative-strand RNA virus, with established safety records in humans, can provide a unique and clinically applicable reprogramming platform.
The balance between the levels of expression of reprogramming factors is important for efficient reprogramming [12,48-50]. For example, Papapetrou et al. have demonstrated that optimal reprogramming efficiency depends on accurate OCT4 dosage and that the relative expression levels of reprogramming factors can affect the efficiency of human iPSC derivation . We found that the levels of OCT4 expression in MV-infected BJ cells was at least 10 times higher than those observed in cells infected with LV(OCT4). We also noticed that the reprograming efficiency with MV + 3LV was lower than with the 4LV. This may be due to the overexpression of OCT4, and further fine-tuning of the expression levels of the four reprogramming factors would improve the reprogramming efficiency. Negative-strand RNA virus transcription is gradually reduced at each gene junction, allowing expression of foreign genes at different levels. Indeed, genomic location was exploited to optimize the expression levels of the KLF4 factor in a Sendai vector .
We have attempted to reprogram BJ cells with an MV expressing SOX2, along with LV encoding OCT4, KLF4, and cMYC. Some of the transduced cells formed neural stem cell-like colonies but not iPSC colonies. SOX2 protein expression analysis indicated substantially higher levels of SOX2 expression from the MV vector when compared with LV(SOX2) (unpublished data). At present, we are further analyzing these clones for potential characteristics of neural stem cells, as SOX2 overexpression can lead to a direct reprogramming into neural stem cells . Reduced SOX2 expression through moving its gene to a downstream location in the MV genome  would improve the iPSC reprogramming.
Measles virus versus Sendai virus vectors
SeV vectors are based on a temperature-sensitive strain, which requires an increase in temperature for a more efficient elimination of the viral genome after reprogramming into iPSCs [11,16]. Without this temperature control, expression of the N protein was monitored in some iPSCs after 30 to 40 passages (Y. Ikeda, unpublished data). Here, we were unable to detect N transcripts after 6 to 8 passages, indicating that viral clearance likely occurs rapidly and that MV vectors might not require an intervention to accelerate elimination. If genome elimination were an issue, we could insert a microRNA target sequence in the MV genome to accelerate virus elimination [53,54]. Another advantage of our new MV vector is that it is deficient in its main immunogenic determinant, the H protein.
Finally, the clinical usage of SeV is limited, including a phase I clinical trial with a SeV vector expressing the human fibroblast growth factor-2 (FGF-2) gene to treat peripheral arterial disease . In contrast, MV has a proven record for safety as a pediatric vaccine, as worldwide MV vaccination prevents an estimated 80 million cases and 4.5 million deaths annually [55,56] with minimal adverse events (on average, less than 10 in 1 million doses) . Additionally, several cancer clinical trials using escalating doses of modified recombinant MV as oncolytic agents have been performed without recording severe adverse events [35,36]. Thus, a novel MV reprogramming system could be directly translated into the clinic, and we are currently working on developing a full MV system to reprogram iPSCs.
In this study, our goal was to provide a proof of principle that MV can be used as a vector for nuclear reprogramming, and now we have opened the door for the development of increasingly efficient vectors and promising avenues for regenerative medicine.
additional transcription unit
Dulbecco’s modified Eagle’s medium
fetal calf serum
green fluorescent protein
induced pluripotent stem cell
multiplicity of infection
reverse transcription-polymerase chain reaction
severe combined immunodeficiency
saponin permeabilization buffer
- TCID50 :
tissue culture infectious dose 50
We thank the Mayo Clinic Cancer Center for the use of the Cytogenetics Core, which provided karyotyping analysis services. The Mayo Clinic Cancer Center is supported in part by a National Cancer Institute Cancer Center Support Grant 5P30 CA15083-36. We thank Ryan A. Knudson and Patricia T. Greipp for cytogenetic analysis. This work was supported by the Mayo Center for Regenerative Medicine (PD) and the National Institutes of Health (R21AI105233 to PD).
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