Isolation and characterization of equine native MSC populations
© The Author(s). 2017
Received: 31 January 2017
Accepted: 28 February 2017
Published: 18 April 2017
In contrast to humans in which mesenchymal stem/stromal cell (MSC) therapies are still largely in the clinical trial phase, MSCs have been used therapeutically in horses for over 15 years, thus constituting a valuable preclinical model for humans. In human tissues, MSCs have been shown to originate from perivascular cells, namely pericytes and adventitial cells, which are identified by the presence of the cell surface markers CD146 and CD34, respectively. In contrast, the origin of MSCs in equine tissues has not been established, preventing the isolation and culture of defined cell populations in that species. Moreover, a comparison between perivascular CD146+ and CD34+ cell populations has not been performed in any species.
Immunohistochemistry was used to identify adventitial cells (CD34+) and pericytes (CD146+) and to determine their localization in relation to MSCs in equine tissues. Isolation of CD34+ (CD34+/CD146–/CD144–/CD45–) and CD146+ (CD146+/CD34–/CD144–/CD45–) cell fractions from equine adipose tissue was achieved by fluorescence-activated cell sorting. The isolated cell fractions were cultured and analyzed for the expression of MSC markers, using qPCR and flow cytometry, and for the ability to undergo trilineage differentiation. Angiogenic properties were analyzed in vivo using a chorioallantoic membrane (CAM) assay.
Both CD34+ and CD146+ cells displayed typical MSC features, namely growth in uncoated tissue culture dishes, clonal growth when seeded at low density, expression of typical MSC markers, and multipotency shown by the capacity for trilineage differentiation. Of note, CD146+ cells were distinctly angiogenic compared with CD34+ and non-sorted cells (conventional MSCs), demonstrated by the induction of blood vessels in a CAM assay, expression of elevated levels of VEGFA and ANGPT1, and association with vascular networks in cocultures with endothelial cells, indicating that CD146+ cells maintain a pericyte phenotype in culture.
This study reports for the first time the successful isolation and culture of CD146+ and CD34+ cell populations from equine tissues. Characterization of these cells evidenced their distinct properties and MSC-like phenotype, and identified CD146+ cells as distinctly angiogenic, which may provide a novel source for enhanced regenerative therapies.
KeywordsPericyte Equine Horse Adventitial cells CD146 CD34 Adipose tissue
Other than humans, horses are the species in which interest in the use of mesenchymal stem/stromal cells (MSCs) for regenerative medicine has been highest [1–8]. Similar to humans, adipose tissue and bone marrow have been the MSC sources of choice in the horse, due to their relative ease of access and the number of putative stem cells they contain [2, 9–13]. Indeed, MSCs have been safely applied clinically to horses for well over a decade, now being an integral component of equine health care worldwide which has an estimated economic impact in the United States and the United Kingdom of $102 billion and $10 billion per annum, respectively. Importantly, given the similarities in the types of natural injuries suffered by both humans and horses, the US Food and Drug Administration  has approved the use of horses as a preclinical model for musculoskeletal conditions.
In regenerative medicine, cell preparations are applied either as crude extracts or following expansion in culture to attain the desired amount of clinical cells. However, these preparations are heterogeneous in nature, containing small and variable amounts of true stem cells in addition to other, nonprecursor cell types which upon transplantation into patients may reduce therapeutic efficacy. There is therefore a real need to identify specific subsets of cells that have intrinsic regenerative capacity, and to develop strategies to robustly isolate, expand in culture, and characterize these cells. However, there is a fundamental lack of understanding of the precise identity and defining features of MSCs in equine tissues, which has thwarted efforts to selectively and efficiently identify, harvest, and expand these cell subpopulations in culture.
Studies in humans have identified two distinct perivascular cell subpopulations, pericytes in capillaries and microvessels [15, 16] and adventitial cells in arteries and veins , as in vivo counterparts of MSCs [18, 19]. Pericytes and adventitial cells were isolated based on the presence of the cell surface markers CD146 and CD34, respectively, and were shown to maintain a typical MSC phenotype in culture. Although tissue regeneration potential has been demonstrated for each individual cell subpopulation [20–22], the properties of CD146 and CD34 cells have not been compared directly.
Using fluorescence-activated cell sorting (FACS), we report the isolation, culture, and characterization of equine CD34+/CD146–/CD144–/CD45– (adventitial cells) and CD146+/CD34–/CD144–/CD45– (pericytes) fractions—for simplicity, referred to hereafter as CD34+ and CD146+, respectively. Both cell types displayed an MSC phenotype, although comparison with non-sorted cells (conventional cultured MSCs) revealed differences in expression of cell surface markers and angiogenic genes. Remarkably, CD146+ cells were distinctly able to promote angiogenesis in vivo, highlighting their potential for tissue regeneration.
Tissue samples were obtained from a total of 15 adult horses immediately post mortem at the School of Veterinary Studies of the University of Edinburgh. All animal procedures were carried out according to the UK Home Office Animals (Scientific Procedures) Act 1986 with approval by the Ethical Review Committee, University of Edinburgh, 60/4207. Adipose tissue was kept for up to 24 h at 4 °C  before extraction of the stromal vascular fraction (SVF).
Frozen samples, processed immediately after tissue harvesting, were cut in a Leica CM1900 cryostat. For staining, tissues were fixed in ice-cold acetone:methanol (50:50) and antibodies were prepared in antibody diluent (003118; Invitrogen-Thermo Fisher Scientific, Paisley, UK). After staining, slides were mounted in Fluoroshield with 4′,6-diamidino-2-phenylindole (DAPI;F6057; Sigma, St Louis, MO, USA). Primary antibodies used were CD146-FITC (MCA2141F; AbD Serotec-BioRad, Kidlington, UK), CD144 (AHP628Z; AbD Serotec-BioRad), NG2 (MAB2585; R&D Systems, Minneapolis, MN, USA), αSMA (ab5694; AbCam, Cambridge, UK), CD29 (303015; BioLegend, San Diego, CA, USA), CD44 (AbD Serotec-BioRad), and CD34 (21270341S; ImmunoTools, Friesoythe, Germany). Isotype controls were IgG1-FITC (MCA928F; AbD Serotec-BioRad), IgG1 (MAB002; R&D Systems), IgG1κ (400101; BioLegend), and IgG1 (21335011; ImmunoTools), all raised in mouse; and rabbit IgG (PRABP01; AbD Serotec-BioRad). Secondary antibodies were conjugated to AF488 (A11029), AF568 (A110037), and AF568 (A10042), all from Invitrogen-Thermo Fisher Scientific. Micrographs were produced using a Zeiss LSM710 confocal or Leica DMLB fluorescent microscope.
Cell extraction and culture
where T is the incubation time in days, X b is the cell number at the beginning of the incubation time, and Xe is the cell number at the end of the incubation time. To determine colony-forming unit fibroblasts (CFU-F), cells were seeded at a density of 1, 5, and 25 cells/cm2 and allowed to grow for 12 days. Colonies were visualized by staining with crystal violet (0.5%) and counted. To obtain endothelial cells, blood vessel sections were washed with PBS and then filled with collagenase II solution (1 mg/ml) before both ends of the vessel were ligated. Following 45-min incubation at 37 °C, endothelial cells were recovered and were cultured in EGM-2 endothelial cell medium.
Flow cytometry and FACS
In order to isolate cell subpopulations, SVFs were stained for 1 h on ice using primary antibodies against CD144 (AHP628Z; AbD Serotec-BioRad), CD45-FITC (MCA832F; BioRad), CD146-AF647 (MCA2141A647; AbD Serotec-BioRad), and CD34-PE (4H11[APG]; ImmunoTools) followed by incubation for 30 min on ice with AF405-conjugated secondary antibody (ab175654; AbCam). Isotype controls used (raised in mouse) were IgG1-FITC (MCA928F; AbD serotec-BioRad), IgG1-AF647 (MCA928A647, AbD serotec-BioRad), and IgG1-PE (21275514; ImmunoTools). Fluorescence minus one (FMO) for CD146 was obtained with a mix of antibodies against CD144, CD45, and CD34, and the mix for CD34 FMO included CD144, CD45, and CD146. DAPI was added to the samples 5 min before flow analysis and was used to determine cell viability. Cells were sorted on a BD FACSAria Fusion (BD Biosciences, San Jose, CA, USA).
In order to analyze the immunophenotype of cultured cells, non-sorted, CD34+, and CD146+ cells were harvested using accutase (SLBN3817V; Sigma). The staining protocol was similar to that already described for FACS and included primary antibodies to CD29 (303001; BioLegend), CD44 (MCA1082GA; AbD serotec-BioRad), CD90 (554895; BD Biosciences), and CD105 (MCA1557T; AbD serotec-BioRad), as well as the isotype controls mouse IgG1κ (400101; BioLegend) and mouse IgG1 (MAB002: R&D Systems). Secondary antibody was AF488-conjugated (A11029; Invitrogen-Thermo Fisher Scientific), and Sytox blue (S34857; Thermo Fisher Scientific) was used to determine cell viability. Samples were run on a BD LSR Fortessa (BD Biosciences) and data were analyzed using BD FACSDiva software version 8.0.1 or FlowJo (LLC, Ashland, OR, USA).
RNA extraction and gene expression analyses
Cultured cells or freshly collected SVFs were harvested in Trizol (15596-026; Invitrogen-Thermo Fisher Scientific) and the concentration of total RNA was measured using Nanodrop. RNA was reverse transcribed with SuperScript III (18080-044; Invitrogen-Thermo Fisher Scientific) and qPCR was performed using the SensiFAST SYBR Lo-ROX kit (BIO-94020; Bioline, London, UK) in a Stratagene machine. Primers were either designed by us for CD144 (5′-TCTGCAGGACATCAATGACAAC-3′ and 5′-CTTCAG GCACGGCAAATACG-3′) and 18S (5′-GCTGGCACCAGACTTG-3′ and 5′-GGGGAATCAGGGTTCG-3′) or were based on previously reported sequences . Data were analyzed with MxPro software and normalized to 18S.
Non-sorted, CD34+, and CD146+ cultured cells were differentiated into adipocytes, chondrocytes, and osteocytes. Briefly, for adipocyte differentiation, cells were grown until 70% confluency [25, 26] and induced in the presence of dexamethasone (1 μm, D4902; Sigma Aldrich), 3-isobutyl-1-methylxanthine (0.5 mM, 15879; Sigma Aldrich), insulin (10 μg/ml, 19278; Sigma Aldrich), indomethacin (100 μM, I8280; Sigma Aldrich), rabbit serum (7%), and fetal bovine serum (3%). Cells were differentiated for 5 days with medium being changed twice. Chondrogenesis and osteogenesis were performed using StemPro differentiation kits following the manufacturer’s instructions for 18 days (A10071-01 and A10072-01, respectively; Life technologies-Thermo Fisher Scientific). Adipocytes were stained with Oil red O (O0625; Sigma-Aldrich) as described before . In brief, cells were first fixed in paraformaldehyde (PFA; 4%) for 3 min, rinsed with 60% isopropanol, and stained with Oil red O (0.1%) for 10 min and then rinsed with isopropanol again and washed with water. Chondrocyte pellets were embedded in Histogel (HG-4000; Invitrogen-Thermo Fisher Scientific) and paraffined, and then cut at 8 μm thickness. Slides were dewaxed and rehydrated. Alcian blue solution (1%) was added to the slide and incubated overnight. After washing, Neutral red (1%) solution was added and absolute ethanol and xylene were used to dehydrate the slides that were mounted with pertex. For osteocyte staining, cells were washed with PBS, fixed with PFA (4%) for 15 min, and stored in PBS at 4 °C. After washing the cells with water, Alizarin Red S (A5533; Sigma-Aldrich) was added. The plate was incubated at room temperature in the dark for 10 min and washed with water. Alkaline phosphatase activity was determined in differentiated cells using a commercial kit (86R-1KT; Sigma-Aldrich) according to the manufacturer’s specifications. Briefly, cells were washed twice with PBS, then fixed with PFA-citrate acetate buffer for 30 sec and washed again with PBS, to which the alkaline–dye mixture was added for the reaction to proceed with wells protected from light, and then washed with water. Negative controls were produced with nondifferentiated cells in culture and micrographs were taken on an Axiovert 25 inverted microscope (Zeiss, Oberkochen, Germany).
The chorioallantoic membrane (CAM) assay was used to assess in vivo angiogenic potential of non-sorted cells and sorted CD34+ and CD146+ cells. Fertilized eggs (NovoGen, Le Foeil, France) were cleaned with ethanol (70%) and incubated at 37 °C in 60% humidity. Three days later, 2 ml of albumen was aspirated from the egg with a syringe and a window was created which was covered again and returned to incubation. On day 8 of incubation, polypropylene rings were placed on the CAM and cells (75,000 cells) or PBS were placed in the center of the ring, as described previously [28, 29]. The eggs were incubated for a further 2 days, when images from each ring were taken using the Zeiss AXIO ZoomV16 microscope at 40×. Binary images were generated by Image J and angiogenesis was quantified using AngioSys2.0 software (Cellworks, Buckingham, UK).
For in vitro angiogenesis experiments, CD146+ and endothelial cells were stained with PKH26 (20 μM) and PKH67 (20 μM) (both from Sigma-Aldrich), producing red and green fluorescence signals, respectively. Once labeled, cells were resuspended in EGM-2 medium and seeded on ibiTreat μ-Slides (IB-81506; Thistle Scientific) coated with matrigel. Pictures were taken using a Zeiss Live Cell Observer/Deconvolution system.
Results are shown as mean ± standard error of the mean, and were analyzed by one-way or two-way ANOVA followed by comparison among means with Tukey test using GraphPad Prim 6.0 software (GraphPad Software, La Jolla, CA, USA). Significance was set at p < 0.05.
CD146 and CD34 mark perivascular cells and colocalize with MSC markers in equine tissues
Isolation of CD146+ and CD34+ cells from equine adipose tissue
CD146+ and CD34+ cells show similar growth in culture
Expression of cell surface markers by CD146+ and CD34+ cells in culture
CD146+ and CD34+ cells express MSC markers
In agreement with the qPCR results, all MSC markers tested by flow cytometry (CD29, CD44, CD90, and CD105) were present in CD34+ and CD146+ cells (Fig. 5b). Importantly, for all markers the antibody-positive cells produced distinct peaks from the isotype controls, indicating that the great majority of the cells in the samples expressed the respective maker. However, differences were observed in mean fluorescence intensity (MFI), suggesting differences in antigen level between cell types (Fig. 5c). In general, MFI values for MSC markers were lower in CD146+ cells than in non-sorted and CD34+ cells, although these differences were not significant in the case of CD44.
CD146+ and CD34+ cells are multipotent
Equine CD146+ cells promote angiogenesis
Despite the enormous interest in the use of MSC therapies in both humans and horses, and the impressive amount of literature devoted to this topic over the last decade, cell heterogeneity in clinical MSC preparations has significantly hindered the full exploitation of these therapies. The identification of two distinct perivascular cell subpopulations, based on the expression of CD146 and CD34, as precursors of MSCs in human tissues [15, 17] provided a significant step forward toward new therapies with increased clinical efficacy. However, studies using defined cell subpopulations (as opposed to traditional heterogeneous MSC preparations) in the horse are lacking, largely a consequence of the difficulty in translating cell isolation procedures developed for human cells to other species and the limited availability of species-specific antibodies. Here, we have successfully established for the first time a methodology to isolate perivascular CD34+/CD146–/CD45–/CD144– and CD146+/CD34–/CD45–/CD144– populations in the horse. We report the successful expansion of these cells in culture as well as the characterization of their properties relative to non-sorted MSC populations.
The observation that CD146 and CD34 have a perivascular location in different equine tissues which coincides with that of established MSC markers is consistent with the notion that pericytes and adventitial cells are native MSC precursors [15, 17, 37], thus extending previous observations in humans to the horse. Our finding that CD146 is robustly maintained by sorted CD146+ cells in culture is in agreement with two previous PCR  and flow cytometry  studies reporting the presence of CD146 in equine MSCs. In contrast, expression of CD34 in CD34+ cells was variable and quickly decreased upon culture, as reported for other cells [31, 32]. This may account in part for the fact that CD34 has only occasionally been detected by PCR in equine MSCs [38, 40], and for the difficulty so far in identifying CD34 antibodies cross-reacting with equine cells. Indeed, in the present study only one of the five CD34 antibodies tested could be used successfully with equine samples.
CD34+ and CD146+ populations displayed typical MSC properties, namely adherence to uncoated tissue-culture dishes, inclusion of CFU-F, and trilineage differentiation, in addition to expressing classical MSC markers both in tissues and during culture. Importantly, flow cytometry analyses indicated that these markers were present in almost all cells within the two sorted subpopulations, although corresponding MFI and mRNA levels were in general lower in CD146+ cells than in the other cell types analyzed. This could be attributed to loss of cell surface marker expression during the time in culture. Another possibility is that cell contaminants such as hematopoietic and/or endothelial cells, which can also express MSC markers [41–45], contributed to the relatively higher expression of these markers, particularly in non-sorted cell cultures. Consistent with this assumption, the single non-sorted cell sample with detectable CD45 mRNA also displayed the highest values for CD90 and CD105 and, in addition, the endothelial marker CD144 was also expressed by non-sorted cells in culture. Regardless of the reasons for these differences, the results indicate that CD146+ cells are distinct from non-sorted and CD34+ cells in equine adipose tissue.
Angiogenesis has a critical contribution to tissue regeneration by allowing supply of oxygen, nutrients, and immune cells and clearing of metabolic products [46, 47]. Because of their anatomical location, pericytes and adventitial cells are uniquely positioned to regulate angiogenesis both by direct contact with endothelial cells and by paracrine production of angiogenic factors [23, 48, 49]. Using the CAM model, we showed that, under our experimental conditions, CD146+ cells, but not non-sorted or CD34+ cells, were able to promote angiogenesis in vivo, presumably due in part to their higher expression of the angiogenic factors VEGFA and ANGPT1. In the horse, two publications [50, 51] reported on the angiogenic activity of MSCs, specifically on the stimulation of vascular network formation by peripheral blood-derived MSCs in culture  and the proangiogenic effects of adipose MSC-derived membrane vesicles in rat aortic rings and scratch assays . However, in those reports the effects of MSCs in vivo were not determined and, in addition, the specific subpopulations involved in the responses observed in vitro were not identified. Limited studies in humans have gone further to determine the angiogenic effects of isolated CD146+ or CD34+ populations in vivo using animal models. In particular, human CD146+ cells were shown to promote angiogenesis after transplantation into SCID/mdx mouse muscle  and in SCID mouse ischemic myocardium . Moreover, improvement of angiogenesis in ischemia was reported in response to CD34+/CD31– cells obtained from human saphenous vena; however, other cell types in these preparations could have contributed to the effects observed . Importantly, the effects of CD146+ and CD34+ on angiogenesis had so far not been directly compared in any species. Our results using the equine model show CD146+ cells to be distinctly angiogenic compared with CD34+ cells and suggest that the former subpopulation is a primary contributor to the proangiogenic properties attributed to MSCs.
Here we report the isolation and characterization of CD146+ and CD34+ cell subpopulations from equine adipose tissue. The results showed that although both subpopulations display a MSC-like phenotype, CD146+ cells are distinctly angiogenic in vivo, indicating CD146+ cells maintain a pericyte phenotype during culture. Based on these results, the marker panels currently used to define MSC populations with regenerative potential may have to be reassessed, because high levels of CD146, but not the classical MSC markers, were associated with angiogenic capacity in this study. Our results also underscore the potential advantages of using defined cell populations over standard heterogeneous MSC preparations for therapy. Angiogenesis is indeed a major component of tissue repair and the use of CD146+ cell subpopulations may be particularly beneficial in the future to improve the efficacy of regenerative treatments both in horses and humans.
Colony-forming unit fibroblasts
Fluorescence-activated cell sorting
Fluorescence minus one
Mean fluorescence intensity
Mesenchymal stem/stromal cells
Stromal vascular fraction
The authors would like to thank Dr John Keen and Dr Ruth Morgan for providing tissue samples and Mr Neil MacIntyre and the staff at the Pathology Services in the Royal (Dick) School of Veterinary Studies for their help.
This work was supported by the Horserace Betting Levy Board (Prj768) and an Institute Strategic Programme Grant from The Biotechnology and Biological Sciences Research Council.
Availability of data and materials
CLE, TAS, SPM, JJP, TM, and LD performed all experiments and collected data. CLE and FXD analyzed the data. CLE, BP, and FXD conceived and design the project and wrote the manuscript. All authors approved the final manuscript.
BP is coinventor of human perivascular stem cell-related patents filed from University of California, Los Angeles. All authors declare they have no competing interests.
Consent for publication
All authors have approved the manuscript and agree to submit to this journal.
All animal procedures were carried out according to the UK Home Office Animals (Scientific Procedures) Act 1986 (60/4207) with approval by the Ethical Review Committee, University of Edinburgh.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Donadeu FX, Esteves CL. Stem cells and equine health. Euro Stem Cells. 2015. http://www.eurostemcell.org/stem-cells-and-equine-health. Accessed 27 Feb 2015.
- Smith RKW, Garvican ER, Fortier LA. The current ‘state of play’ of regenerative medicine in horses: what the horse can tell the human. Regen Med. 2014;9:673–85.View ArticlePubMedGoogle Scholar
- Burk J, Badylak SF, Kelly J, et al. Equine cellular therapy—from stall to bench to bedside? Cytom Part A. 2013;83A:103–13.View ArticleGoogle Scholar
- Ranera B, Barry F. A horse of a different color. Cytom Part A. 2014;85:658–9.View ArticleGoogle Scholar
- Brehm W, Burk J, Delling U. Application of stem cells for the treatment of joint disease in horses. Methods Mol Biol. 2014;1213:215–28.View ArticlePubMedGoogle Scholar
- Donadeu FX, Esteves CL. Prospects and challenges of induced pluripotent stem cells in equine health. Front Vet Sci. 2015;2:59.View ArticlePubMedPubMed CentralGoogle Scholar
- Breton A, Sharma R, Diaz AC, et al. Derivation and characterization of induced pluripotent stem cells from equine fibroblasts. Stem Cells Dev. 2013;22:611–21.View ArticlePubMedGoogle Scholar
- Sharma R, Livesey MR, Wyllie DJ, et al. Generation of functional neurons from feeder-free, keratinocyte-derived equine induced pluripotent stem cells. Stem Cells Dev. 2014;23:1524–34.View ArticlePubMedGoogle Scholar
- Burk J, Ribitsch I, Gittel C, et al. Growth and differentiation characteristics of equine mesenchymal stromal cells derived from different sources. Vet J. 2013;195:98–106.View ArticlePubMedGoogle Scholar
- Smith R. Mesenchymal stem cell therapy for equine tendinopathy. Disabil Rehabil. 2008;30:1752–8.View ArticlePubMedGoogle Scholar
- Ranera B, Ordovas L, Lyahyai J, et al. Comparative study of equine bone marrow and adipose tissue-derived mesenchymal stromal cells. Equine Vet J. 2012;44:33–42.View ArticlePubMedGoogle Scholar
- De Schauwer C, Meyer E, Van de Walle GR, et al. Markers of stemness in equine mesenchymal stem cells: a plea for uniformity. Theriogenology. 2011;75:1431–43.View ArticlePubMedGoogle Scholar
- Esteves CL, Sharma R, Dawson L, et al. Expression of putative markers of pluripotency in equine embryonic and adult tissues. Vet J. 2014;202:533–5.View ArticlePubMedGoogle Scholar
- Sah RL, Ratcliffe A. Translational Models for Musculoskeletal Tissue Engineering and Regenerative Medicine. Tissue Eng Part B. 2010;16(1):1-3.
- Crisan M, Yap S, Casteilla L, et al. A perivascular origin for mesenchymal stem cells in multiple human organs. Cell Stem Cell. 2008;3:301–13.View ArticlePubMedGoogle Scholar
- Crisan M, Chen C-W, Corselli M, et al. Perivascular multipotent progenitor cells in human organs. Ann New Y Acad Sci. 2009;1176:118–23.View ArticleGoogle Scholar
- Corselli M, Chen CW, Sun B, et al. The tunica adventitia of human arteries and veins as a source of mesenchymal stem cells. Stem Cells Dev. 2012;21:1299–308.View ArticlePubMedGoogle Scholar
- Dominici M, Le B, Mueller I, et al. Minimal criteria for defining multipotent mesenchymal stromal cells. The international society for cellular therapy position statement. Cytotherapy. 2006;8:315–7.View ArticlePubMedGoogle Scholar
- Bourin P, Bunnell BA, Casteilla L, et al. Stromal cells from the adipose tissue-derived stromal vascular fraction and culture expanded adipose tissue-derived stromal/stem cells: a joint statement of the International Federation for Adipose Therapeutics and Science (IFATS) and the International Society for Cellular Therapy (ISCT). Cytotherapy. 2013;15:641–8.View ArticlePubMedPubMed CentralGoogle Scholar
- James AW, Zara JN, Corselli M, et al. An abundant perivascular source of stem cells for bone tissue engineering. Stem Cells Transl Med. 2012;1:673–84.View ArticlePubMedPubMed CentralGoogle Scholar
- Tawonsawatruk T, West CC, Murray IR, et al. Adipose derived pericytes rescue fractures from a failure of healing—non-union. Sci Rep. 2016;6:22779.View ArticlePubMedPubMed CentralGoogle Scholar
- Chen CW, Okada M, Proto JD, et al. Human pericytes for ischemic heart repair. Stem Cells. 2013;31:305–16.View ArticlePubMedPubMed CentralGoogle Scholar
- West CC, Hardy WR, Murray IR, et al. Prospective purification of perivascular presumptive mesenchymal stem cells from human adipose tissue: process optimization and cell population metrics across a large cohort of diverse demographics. Stem Cell Res Ther. 2016;7:47.View ArticlePubMedPubMed CentralGoogle Scholar
- Ranera B, Lyahyaia J, Romero A, et al. Immunophenotype and gene expression profiles of cell surface markers of mesenchymal stem cells derived from equine bone marrow and adipose tissue. Vet Immunol Immunopathol. 2011;144:147–54.View ArticlePubMedGoogle Scholar
- Esteves CL, Kelly V, Breton A, et al. Proinflammatory cytokine induction of 11beta-hydroxysteroid dehydrogenase type 1 (11beta-HSD1) in human adipocytes is mediated by MEK, C/EBPbeta, and NF-kappaB/RelA. J Clin Endocrinol Metab. 2014;99:E160–8.View ArticlePubMedGoogle Scholar
- Esteves CL, Kelly V, Bégay V, et al. Regulation of adipocyte 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) by CCAAT/Enhancer-Binding Protein (C/EBP) β isoforms. LIP and LAP. PLoS One. 2012;7, e37953.View ArticlePubMedPubMed CentralGoogle Scholar
- Esteves CL, Kelly V, Bégay V, et al. Stable conditional expression and effect of C/EBPβ-LIP in adipocytes using the pSLIK system. J Mol End. 2013;51:91–8.View ArticleGoogle Scholar
- Ribatti D, Nico B, Vacca A, et al. The gelatin sponge-chorioallantoic membrane assay. Nat Protoc. 2006;1:85–91.View ArticlePubMedGoogle Scholar
- Dohle DS, Pasa SD, Gustmann S, et al. Chick ex ovo culture and ex ovo CAM assay: how it really works. J Vis Exp. 2009;33:1620.Google Scholar
- Zimmerlin L, Donnenberg VS, Pfeifer ME, et al. Stromal vascular progenitors in adult human adipose tissue. Cytom Part A. 2010;77:22–30.Google Scholar
- Sidney LE, Branch MJ, Dunphy SE et al. Concise review: Evidence for CD34 as a common marker for diverse progenitors. Stem Cells. 2014
- Braun J, Kurtz A, Barutcu N, et al. Concerted regulation of CD34 and CD105 accompanies mesenchymal stromal cell derivation from human adventitial stromal cell. Stem Cells Dev. 2013;22:815–27.View ArticlePubMedGoogle Scholar
- Radcliffe CH, Flaminio MJ, Fortier LA. Temporal analysis of equine bone marrow aspirate during establishment of putative mesenchymal progenitor cell populations. Stem Cells Dev. 2010;19:269–82.View ArticlePubMedPubMed CentralGoogle Scholar
- Edwards SS, Zavala G, Prieto CP, et al. Functional analysis reveals angiogenic potential of human mesenchymal stem cells from Wharton's jelly in dermal regeneration. Angiogenesis. 2014;17:851–66.View ArticlePubMedGoogle Scholar
- Bronckaers A, Hilkens P, Fanton Y, et al. Angiogenic properties of human dental pulp stem cells. PLoS One. 2013;8, e71104.View ArticlePubMedPubMed CentralGoogle Scholar
- Wang C-H, Wang T-M, Young T-H, et al. The critical role of ECM proteins within the human MSC niche in endothelial differentiation. Biomaterials. 2013;34:4223–34.View ArticlePubMedGoogle Scholar
- Sorrentino A, Ferracin M, Castelli G, et al. Isolation and characterization of CD146+ multipotent mesenchymal stromal cells. Exp Hematol. 2008;36:1035–46.View ArticlePubMedGoogle Scholar
- Ranera B, Lyahyai J, Romero A, et al. Immunophenotype and gene expression profiles of cell surface markers of mesenchymal stem cells derived from equine bone marrow and adipose tissue. Vet Immunol Immunopathol. 2011;144:147–54.View ArticlePubMedGoogle Scholar
- Hoynowski S, Fry M, Gardner B, et al. Characterization and differentiation of equine umbilical cord-derived matrix cells. Biochem Biophys Res Commun. 2007;362:347–53.View ArticlePubMedGoogle Scholar
- Lange-Consiglio A, Corradetti B, Bizzaro D, et al. Characterization and potential applications of progenitor-like cells isolated from horse amniotic membrane. J Tissue Eng Regen Med. 2012;6:622–35.View ArticlePubMedGoogle Scholar
- Mettouchi A, Meneguzzi G. Distinct roles of β1 integrins during angiogenesis. Eur J Cell Biol. 2006;85:243–7.View ArticlePubMedGoogle Scholar
- Zhang B. CD73: a novel target for cancer immunotherapy. Cancer Res. 2010;70:6407–11.View ArticlePubMedPubMed CentralGoogle Scholar
- Narravula S, Lennon PF, Mueller BU, et al. Regulation of endothelial CD73 by adenosine: paracrine pathway for enhanced endothelial barrier function. J Immunol. 2000;165:5262–8.View ArticlePubMedGoogle Scholar
- Nassiri F, Cusimano MD, Scheithauer BW, et al. Endoglin (CD105): a review of its role in angiogenesis and tumor diagnosis. progression and therapy. Anticancer Res. 2011;31:2283–90.PubMedGoogle Scholar
- Hofmeister V, Schrama D, Becker JC. Anti-cancer therapies targeting the tumor stroma. Cancer Immunol Immunother. 2008;57:1–17.View ArticlePubMedGoogle Scholar
- Carmeliet P. Angiogenesis in health and disease. Nat Med. 2003;9:653–60.View ArticlePubMedGoogle Scholar
- Auerbach R, Lewis R, Shinners B, et al. Angiogenesis assays: a critical overview. Clin Chem. 2003;49:32–40.View ArticlePubMedGoogle Scholar
- Murphy MB, Moncivais K, Caplan AI. Mesenchymal stem cells: environmentally responsive therapeutics for regenerative medicine. Exp Mol Med. 2013;45, e54.View ArticlePubMedPubMed CentralGoogle Scholar
- Murray IR, West CC, Hardy WR, et al. Natural history of mesenchymal stem cells, from vessel walls to culture vessels. Cell Mol Life Sci. 2014;71:1353–74.View ArticlePubMedGoogle Scholar
- Bussche L, Van de Walle GR. Peripheral blood-derived mesenchymal stromal cells promote angiogenesis via paracrine stimulation of vascular endothelial growth factor secretion in the equine model. Stem Cells Transl Med. 2014;3:1514–25.View ArticlePubMedPubMed CentralGoogle Scholar
- Pascucci L, Alessandri G, Dall'Aglio C, et al. Membrane vesicles mediate pro-angiogenic activity of equine adipose-derived mesenchymal stromal cells. Vet J. 2014;202:361–6.View ArticlePubMedGoogle Scholar
- Park TS, Gavina M, Chen CW, et al. Placental perivascular cells for human muscle regeneration. Stem Cells Dev. 2011;20:451–63.View ArticlePubMedGoogle Scholar
- Campagnolo P, Cesselli D, Al Haj Zen A, et al. Human adult vena saphena contains perivascular progenitor cells endowed with clonogenic and proangiogenic potential. Circulation. 2010;121:1735–45.View ArticlePubMedPubMed CentralGoogle Scholar