Open Access

Biophysical regulation of stem cell behavior within the niche

Stem Cell Research & Therapy20123:50

DOI: 10.1186/scrt141

Published: 14 December 2012

Abstract

Stem cells reside within most tissues throughout the lifetimes of mammalian organisms. To maintain their capacities for division and differentiation and thereby build, maintain, and regenerate organ structure and function, these cells require extensive and precise regulation, and a critical facet of this control is the local environment or niche surrounding the cell. It is well known that soluble biochemical signals play important roles within such niches, and a number of biophysical aspects of the microenvironment, including mechanical cues and spatiotemporally varying biochemical signals, have also been increasingly recognized to contribute to the repertoire of stimuli that regulate various stem cells in various tissues of both vertebrates and invertebrates. For example, biochemical factors immobilized to the extracellular matrix or the surface of neighboring cells can be spatially organized in their placement. Furthermore, the extracellular matrix provides mechanical support and regulatory information, such as its elastic modulus and interfacial topography, which modulate key aspects of stem cell behavior. Numerous examples of each of these modes of regulation indicate that biophysical aspects of the niche must be appreciated and studied in conjunction with its biochemical properties.

Introduction

The concept that the behavior of a stem cell can be modulated by factors in its immediate vicinity arose several decades ago in studies of spleen colony-forming cells, which were later appreciated to be hematopoietic stem and progenitor cells (HSPCs) [1]. It was hypothesized that these HSPCs and their progeny were distinct cell populations that possessed an 'age structure', such that once the progeny left their stem cell niche during developmental 'aging', their stem-like qualities were lost, and entry into a new niche promoted differentiation into a more mature, lineage-committed cell type. Subsequent work with Drosophila germ stem cells [2] and other systems demonstrated that the niche is a region that regulates stem cell fate decisions by presenting that cell with specific repertoires of soluble and immobilized extracellular factors. It is increasingly appreciated that many of these signals are biophysical in nature, particularly biochemical factors that are spatiotemporally modulated, mechanical cues, and electrostatic cues. Over the past several years, numerous examples in which the first two of these properties in particular have been shown to play key regulatory roles have emerged.

Spatial organization of cues in the niche

Many factors that are often thought of as soluble are known to harbor matrix-binding domains that immobilize them to the solid phase of tissue. For example, fibroblast growth factors, platelet-derived growth factors (PDGFs), transforming growth factors (TGFs), vascular endothelial growth factors (VEGFs), Hedgehogs, and numerous cytokines contain heparin-binding domains [36]. Immobilization of such factors to the extracellular matrix (ECM) often modulates their activity by promoting sustained signaling via inhibiting receptor-mediated endocytosis [7], increasing their local concentration and establishing concentration gradients emanating from the source [8], and otherwise modulating the spatial organization of factors in a manner that affects signaling. As an example, compared with soluble VEGF, VEGF bound to collagen preferentially activates VEGFR2, associates with β1 integrins, and promotes the association of all of these molecules into focal adhesions [9]. There are also strong examples of synthetic systems that harness these phenomena, the first of which involved tethering epidermal growth factor to immobilized poly(ethylene oxide) (PEO) to prolong growth factor signaling in rat hepatocyte cultures [10]. A subsequent study showed that immobilization of Sonic hedgehog (Shh) onto inter penetrating polymer network surfaces, along with the integrin-engaging peptide arginine-glycine-asparagine (RGD), induced potent osteoblastic differentiation of bone marrow-derived mesenchymal stem cells (MSCs), whereas soluble Shh enhanced proliferation [11]. As another example, crosslinking heparin-binding peptides to fibrin gels along with neurotrophic factor 3 (NT-3) and PDGF resulted in neuronal and oligodendrocytic differentiation of mouse neural stem cells (NSCs) with inhibition of astrocytic differentiation [12]. Finally, immobilization of leukemia inhibitory factor (LIF) to a synthetic polymer surface supported mouse embryonic stem cell (mESC) pluripotency for up to two weeks in the absence of soluble LIF, indicating the advantage of substrate functionalization in lowering cell culture reagent costs and facilitating future multifactorial cell fate screening experiments [13].

Immobilization of cues to the solid phase - that is, the ECM or the surface of adjacent cells or both - also offers the opportunity to modulate the nanoscale organization in which these factors are presented (Figure 1). Growing evidence has indicated that ligand multivalency, or the number of ligands organized into a nanoscale cluster, can exert potent effects on cell behavior [1417]. For example, seminal work using a synthetic system to present clusters of ECM-derived adhesion ligands showed that the spatial organization of ECM cues can also impact cell responses. Specifically, on surfaces functionalized with the integrin adhesion ligand YGRGD in various states of valency, fibroblast attachment did not vary as a function of ligand valency, yet substrates bearing highly clustered or multivalent peptides required significantly lower ligand densities to induce cell spreading and migration [18]. In recent work that explored the behavior of MSCs in a three-dimensional (3D) hydrogel functionalized with RGD peptides, investigators who used a fluorescence resonance energy transfer technique found that the cells apparently reorganized the peptides into clusters upon integrin binding [19].
https://static-content.springer.com/image/art%3A10.1186%2Fscrt141/MediaObjects/13287_2012_Article_138_Fig1_HTML.jpg
Figure 1

Mechanical and biophysical interactions in the stem cell niche. The native microenvironment, or niche, in which a stem cell resides can be highly complex, consisting of various cell types, extracellular matrix (ECM) molecules, and growth factors. Proteoglycans and ECM proteins bind and immobilize otherwise soluble growth factors, providing functional sites for cell binding as well as mechanical stability of the space surrounding a stem cell in its niche. ECM fibers and neighboring niche cells provide mechanical support and stimuli (short red arrows) to influence stem cell fate. The degree of 'crosslinking' of the various ECM molecules also affects the pore size in the niche, dictating the rate of diffusion of soluble factors as well as the ability of niche cells to infiltrate nearby space. Finally, flow through local vasculature (long red arrows) mechanically shears endothelial and other cells (green), which may in turn affect nearby stem cells.

The role of ligand clustering also extends to growth factors and morphogens. The morphogen Hedgehog and its family member Shh, best known for their role in tissue patterning during development, have been shown to require nanoscale clustering to achieve long-range paracrine signaling [20]. Additionally, transforming growth factor-beta (TGF-β) is able to induce distinct differential signaling by activating either a homomeric or a heteromeric form of its receptor, which needs to be dimerized or tetramerized before signaling can occur [21]. Furthermore, cell membrane-bound ligands (for example, Delta/Jagged that activate the Notch receptor and ephrins that activate corresponding Eph receptors) often require oligomerization to transduce biochemical signaling cascades [22, 23]. The creation of synthetically clustered, or multivalent, ligands offers a useful tool to study basic biological aspects of receptor clustering as well as a reagent to better control stem cell self-renewal or differentiation. For example, Shh has been chemically conjugated to the long polymer chain hyaluronic acid at varying stochiometric ratios to produce a range of multivalent forms of Shh, and higher-valency Shh bioconjugates exerted progressively higher potencies in inducing the osteogenic differentiation of a primary fibroblast line with MSC characteristics [24]. This concept was recently extended to create highly active and multivalent versions of ligands that are naturally integral membrane proteins (A Conway, T Vazin, N Rode, KE Healy, RS Kane, DV Schaffer, unpublished data).

In addition to spatial regulation of cues at the nanoscale, microscale features in the niche can play key roles. Fibrous ECM proteins such as collagen and fibronectin are present throughout the NSC niche, raising the hypothesis that cells may respond to ECM surface topography. One interesting demonstration of this idea showed that rat NSCs cultured on laminin-coated synthetic polyethersulfone fibers of 280 or 1,500 nm in diameter preferentially differentiated into oligodendrocytes or neurons, respectively. It has also been shown that culturing MSCs atop vertically oriented nanotubes of 70 to 100 nm in diameter (but not less than 30 nm) is sufficient to induce their differentiation into osteoblasts [25]. In an analogous study, culturing MSCs on nanopits of 100 nm also induces osteogenesis but only if the pits are anisotropic, or disordered [26]. Recently, the cytoskeletal scaffolding protein zyxin was shown to play an important role in the response of human MSCs to surface nanotopography [27]. Specifically, MSCs expressed zyxin at lower levels when plated on a polydimethylsiloxane (PDMS) surface patterned with a 350-nm grating, which resulted in smaller and more dynamic focal adhesions and increased directional migration of the cells along the gratings.

In addition to nanoscale features, cell-cell interactions at the microscale affect behavior. Specifically, the assembly of stem cells themselves into multicellular aggregates exerts strong influences on cell self-renewal or differentiation, as the cells actively secrete factors and modulate local biological transport properties in ways that impact their neighbors. For example, several groups have created controlled 3D culture systems to generate human embryonic stem cell (hESC) embryoid bodies (EBs) - or cell clusters - of defined sizes. These involved centrifugal-forced aggregation [28] as well as microfabricated PDMS wells surrounded with functionalized protein-resistant self-assembled monolayers [29]. These methods produced more consistent sizes than EB suspensions, and in the latter example a tighter distribution of EB volume was accompanied by a higher level of expression of the pluripotency marker Oct-4. In another key study, hESC culture inside microfabricated poly(ethylene glycol) (PEG) wells yielded EBs from 40 to 450 μm in diameter [30, 31]. Greater endothelial cell differentiation was observed in smaller EBs (150 μm), which was shown to be due to higher Wnt5a expression, whereas larger EBs (450 μm) enhanced cardiogenesis as a result of higher Wnt11 expression. Interestingly, another group used microcontact printing of adhesive islands on two-dimensional substrates to control hESC colony size and showed that smaller hESC colonies became more endoderm-biased, whereas larger colonies exhibited greater differentiation into neural lineages [32]. Within the endoderm-biased colonies, cardiogenesis was found to be more pronounced in larger EBs as opposed to the neural-biased colonies, which had higher levels of cardiogenesis in smaller EBs. Collectively, these results demonstrate that spatial organization of molecules and cells can play critical roles in modulating stem cell fate and can therefore serve as important tools to exert exogenous control over these processes.

Mechanoregulation in the niche

The mechanical properties of tissues have been studied for a number of decades. In the 1950s, it was observed that cells of the mesenchyme grow preferentially toward regions that are under higher mechanical stress, indicating a fundamental contribution of mechanical properties to biological function [33, 34]. Aberrant tissue-elastic mechanical properties have also been shown to play a pathological role in certain cases, such as causing increased contractility of arterial resistance vessels within hypertensive rats, leading to elevated blood pressure and eventual heart failure [35]. There is a strong rationale for why mechanical properties may also modulate stem cell behavior. Tissues in the body range over several orders of magnitude in stiffness, from the softness of adipose to the toughness of bone, hinting at the possible importance of mechanics in maintaining different adult organs. In addition, there is local heterogeneity within individual tissues, as it has been shown, for example, that the hippocampus - a brain region that harbors adult NSCs - spatially varies in stiffness, as assessed by atomic force microscopy [36]. These various differences are not captured in the hard tissue culture surfaces typically used for in vitro study.

Engler and colleagues [37], in pioneering work, demonstrated that substrate elastic modulus affects stem cell lineage commitment, in which MSCs cultured on polyacrylamide substrates of varying elastic moduli differentiated into cell types characteristic of tissues with the corresponding stiffness: neurons, myoblasts, and osteoblasts. A later study extended this concept to another stem cell type by showing that NSCs cultured on variable modulus substrates differentiate preferentially into neurons on softer substrates and astrocytes on harder materials [38]. Recently, it was shown that soft substrates enhance the ability of human embryonic and human-induced pluripotent stem cells to differentiate into neural lineages [39].

The finding that increased matrix rigidity can modulate cell differentiation has also been extended to analysis of the epithelial-mesenchymal transition (EMT) of both murine mammary gland cells and canine kidney epithelial cells, where more rigid substrates promoted EMT via upregulating the Akt signaling pathway [40]. In addition to differentiation on a single stiffness, durotaxis - the ability of cells to migrate in response to a stiffness gradient - and mechanosensitive differentiation can be integrated. For example, upon seeding of MSCs on a surface with a gradient in stiffness, cells migrated preferentially toward the stiffer region of the gel and then differentiated according to the local stiffness [41]. Finally, stem cells can, in turn, strongly influence their mechanical environment. MSCs cultured on non-linear strain-stiffening fibrin gels have been shown, upon application of local strain via cytoskeletal rearrangement and cell spreading, to globally stiffen the gel [42]. This effect led to long-distance cell-cell communication and alignment, thus indicating that cells can be acutely responsive to the non-linear elasticity of their substrates and can manipulate this rheological property to induce patterning.

In addition to differentiation, modulus can influence stem cell self-renewal. For example, it was shown that substrate stiffness strongly impacts the ability of muscle stem cells, or satellite cells, to undergo self-renewal in culture. Upon implantation, cells isolated from muscle and grown on soft substrates were able to expand and contribute to muscle to a much greater extent than stem cells cultured on stiff surfaces [43]. Furthermore, mESC self-renewal is promoted on soft substrates, accompanied by downregulation cell-matrix tractions [44].

Mechanobiologists have begun to elucidate mechanisms by which stem cells undergo mechanoregulation, building on advances with non-stem cells. Several mechanotransductive proteins involved with producing traction forces via cytoskeletal rearrangements are thought to be implicated in translating mechanical signals into changes in gene expression in stem cells [37, 45, 46]. For example, it has been shown that inhibition of myosin II diminishes the effect of ECM stiffness on MSC differentiation [37]. Furthermore, decreasing ECM stiffness decreases RhoA activity and subsequent calcium signaling in MSCs [47]. Recent work also indicates that Rho GTPases, specifically RhoA and Cdc42, enable NSCs to adjust their own stiffness as a function of the substrate modulus and thereby regulate the cells' stiffness-dependent differentiation into either astrocytes or neurons in vitro and potentially in vivo [46]. Furthermore, an important study demonstrated that the transcriptional coactivator YAP undergoes nuclear localization in MSCs on higher-stiffness substrates, thereby narrowing the gap in our understanding of how microenvironmental mechanical properties may ultimately modulate gene expression and, as a result, cell differentiation [48]. Finally, while mechanosensitive stem cell behavior has been demonstrated on several materials in addition to the original polyacrylamide, recent work broaches another possible mechanism for cell behavior on different stiffnesses. Specifically, investigators found that MSCs exhibited different behavior on polyacrylamide but not PDMS gels of variable modulus, and additionally found that the porosity of the polyacrylamide but not the PDMS gels varied with stiffness. This raised the intriguing possibility that differences in ECM conjugation - specifically the number of anchoring points of collagen to the gel surface - could subsequently affect integrin binding and thereby modulate cell responses [49]. This possibility should be explored further, potentially in comparison with findings that NSCs and MSCs on polyacrylamide-based materials behave similarly as a function of modulus for materials presenting either ECM proteins [37, 46] or simple RGD peptides [19, 38].

In addition to the static mechanical properties of cells and surrounding tissue, dynamic biomechanical processes can regulate stem cell function. For instance, stress and strain from local tissue contraction and expansion, including processes such as contraction of muscle, tendons, and ligaments as well as cyclic deformation of tissue surrounding vasculature and the lungs, are prevalent in vivo. Furthermore, organismal development is a highly dynamic process that exposes cells and structures to mechanical forces. In Drosophila embryos, for example, compression of cells induces expression of Twist, a protein involved with regulating germ layer specification and patterning [50]. Similarly, in zebrafish, tensile strains were shown to regulate gastrulation during early development [51]. Such basic studies extend to mammalian stem cells. For example, cyclic strain of lung embryonic MSCs stimulates expression and nuclear localization of tension-induced/inhibited protein-1 (TIP-1) and inhibits expression of TIP-3, thereby promoting myogenesis and inhibiting adipogenesis [52]. Cyclical stretching also inhibits differentiation of hESCs through upregulation of Nodal, Activin A, and TGFβ1 [53]. Differential effects of equiaxial versus uniaxial strain have also been observed, with equiaxial primarily down regulating smooth muscle cell promoting factors in MSCs and uniaxial upregulating them [54].

Even temporal variation of the ECM on slower timescales may play a role in regulating stem cell function [55]. For example, matrix metalloproteinases (MMPs), enzymes that remodel the ECM through cleavage of key constituent proteins, can modulate stem cell differentiation. Interestingly, it has been shown that, in response to two injury-induced chemokines, SDF-1 and VEGF, NSCs in the subventricular zone of the lateral ventricles in the adult rodent brain differentiated into migratory cells that secreted MMPs at elevated levels [56]. Blocking the expression of these proteins inhibited differentiation of the NSCs, indicating that the cells require matrix remodeling to proceed with their differentiation and subsequent migration into injured areas of the brain. MSCs localized to bone marrow have also been shown to secrete MMPs to facilitate infiltration of sites of tissue damage, inflammation, or neoplasia before undergoing differentiation [57]. In addition to experiencing a decrease in ECM integrity, cells can experience ECM stiffening (for example, an approximately 10-fold increase in stiffness during cardiac maturation). Young and Engler [58] created a hyaluronic acid poly(ethylene glycol) hydrogel that could undergo stiffening over a two-week period and found that pre-cardiac cells within the gel underwent a significantly higher increase in maturation - both expression of muscle markers and assembly into muscle fibers - than corresponding cells seeded on static hydrogels. The development of hydrogels in which crosslinks are photosensitive has enabled investigators to vary stiffness in time and space, powerful capabilities that will enable further advances in the field [59, 60].

Another form of dynamic stress is shear flow, most often associated with the circulatory system. The earliest study of shear on stem cell fate determined that flow promotes maturation and capillary assembly of endothelial progenitor cells [61]. Subsequent studies showed that shear flow can induce differentiation of other stem cell types, including endothelial cell specification from murine embryonic MSCs [62] and vascular endothelial cell lineage commitment from ESCs [63, 64]. Each of these properties and parameters of the niche (summarized in Table 1) offers opportunities to control cell fate for downstream therapeutic application.
Table 1

Examples of biophysical regulation within the stem cell niche

Biophysical property

Stimulus

Cell type

Response

References

Ligand-substrate immobilization

VEGF; EGF; Shh; NT-3, PDGF; LIF, SCF

Human endothelial cells; rat hepatocytes; rat MSCs; hESC-derived NPCs; mESCs

VEGFR2 activation; DNA synthesis; osteoblast differentiation; decreased astrogensis; STAT3/MAPK activation

[9]; [10]; [11]; [12]; [13]

Multivalent presentation

Galactose; RGD; Hh; TGF-β; Shh

Escherichia coli; mouse fibroblasts; Drosophila; mink lung epithelial cells; mouse embryonic C3H10T1/2 cells

Chemotaxis; motility/adhesion; patched activation; endocytosis; osteogenic differentiation

[16]; [18]; [20]; [21]; [24]

Surface topography

70- to 100-nm nanotubes; nanotopographical disorder; 350-nm gratings; decreased collagen-anchoring sites

hMSCs; hMSCs; hMSCs; human epidermal stem cells

Osteoblast differentiation; bone ECM formation; decreased zyxin/increased motility; increased differentiation

[25]; [26]; [27]; [49]

Physical orientation of stem cells

450-μm cell cluster size/150-μm cell cluster size; decreased cell colony size

mESCs; hESCs

Cardiogenesis/endothelial cell differentiation; increased endodermal differentiation

[31]; [32]

Elastic modulus

Soft/hard matrix; decreased substrate stiffness; increased ECM stiffness; decreased/increased matrix rigidity; substrate stiffness gradient; soft hydrogel substrates; soft substrates

hMSCs; rat NPCs; hPSCs; murine mammary gland cells; hMSCs; mMuSCs; mESCs

Neurogenesis/osteogenesis; increased neuronal differentiation; increased cell and colony spreading; TGF-β1-induced apoptosis/EMT; migration up stiffness gradient; self-renewal and in vivo regeneration; homogeneous self-renewal and downregulated cell tractions

[37]; [38]; [39]; [40]; [41]; [43]; [44]

Dynamic mechanical forces

Local cell traction on non-linear elastic fibrin gel; cell compression; cell-cortex tension; stretch-induced TIP-1/TIP-3 expression; cyclic biaxial strain; equiaxial/uniaxial strain; dynamic hydrogel stiffening; shear stress; shear stress; laminar shear stress; fluid shear stress

hMSCs; Drosophila germ cells; zebrafish; lung EMCs; hESCs; human bone marrow MSCs; chicken cardiomyocytes; hEPCs; mEMCs; mESCs; mESCs

Global matrix stiffening; Twist protein expression; progenitor-cell sorting; myogenesis/adipogenesis; increased TGFβ1/Activin A/Nodal expression; SM α-actin and SM-22α downregulation/upregulation; increased cardiac maturation; proliferation, differentiation, and vascular tube formation; endothelial differentiation; epigenetic histone modification and cardiovascular lineage programming; differentiation into vascular endothelial cells

[42]; [50]; [51]; [52]; [53]; [54]; [58]; [61]; [62]; [63]; [64]

ECM, extracellular matrix; EGF, epidermal growth factor; EMC, embryonic mesenchymal cell; EMT, epithelial-mesenchymal transition; hEPC, human endothelial progenitor cell; hESC, human embryonic stem cell; Hh, hedgehog; hMSC, human mesenchymal stem cell; hPSC, human pluripotent stem cell; LIF, leukemia inhibitory factor; MAPK, mitogen-activated protein kinase; mEMC, mouse embryonic mesenchymal cell; mESC, mouse embryonic stem cell; mMuSC, mouse muscle stem cell; MSC, mesenchymal stem cell; NPC, neural progenitor cell; NT-3, neurotrophic factor 3; PDGF, platelet-derived growth factor; RGD, arginine-glycine-asparagine peptide; SCF, stem cell factor; Shh, sonic hedgehog; SM, smooth muscle; STAT3, signal transducer and activator of transcription 3; TGF-β, transforming growth factor-beta; TIP, tension-induced/inhibited protein; VEGF, vascular endothelial growth factor; VEGFR2, vascular endothelial growth factor receptor 2.

Conclusions

Understanding the properties and effects of each complex component of a local stem cell microenvironment is an essential step toward understanding the stem cell itself. In particular, the ability of a stem cell to respond to spatiotemporally varying biochemical cues and distinct mechanical and physical stimuli within its surroundings is being increasingly recognized and will continue to be elucidated in the years to come. The effect of substrate stiffness on stem cell fate has been increasingly appreciated in recent years, and other facets of the niche's solid phase - including spatial organization in the presentation of biochemical information, electrostatics [65], and bio-molecular transport [66] - will increasingly be investigated. While technological limitations in the ability to control, quantify, and image these properties currently exist, advances in super-resolution microscopy may be combined with stem cell research to enable considerable progress [67].

Furthermore, an appreciation of these interactive processes in natural tissue may greatly aid the development of stem cell therapies to treat numerous human diseases. For example, this basic knowledge may enable therapeutic modulation of endogenous stem cells via alterations in the niche as well as offer opportunities to create more effective large-scale culture systems and bioreactors to expand and differentiate stem cells. Furthermore, the creation of in vitro cell and tissue equivalents of therapeutically relevant organs, enabled by the technological advances and optimized model culture systems, will enable both basic and therapeutic investigations of human disease biology. Therefore, as is evidenced by an increasing number of important studies, a blend of biology, chemistry, physics, and engineering can empower progress in both basic and translational directions.

Abbreviations

3D: 

three-dimensional

EB: 

embryoid body

ECM: 

extracellular matrix

EMT: 

epithelial-mesenchymal transition

hESC: 

human embryonic stem cell

HSPC: 

hematopoietic stem and progenitor cell

LIF: 

leukemia inhibitory factor

mESC: 

mouse embryonic stem cell

MMP: 

matrix metalloproteinase

MSC: 

mesenchymal stem cell

NSC: 

neural stem cell

PDGF: 

platelet-derived growth factor

PDMS: 

polydimethylsiloxane

RGD: 

arginine-glycine-asparagine

Shh: 

sonic hedgehog

TGF: 

transforming growth factor

TIP-1: 

tension-induced/inhibited protein-1

VEGF: 

vascular endothelial growth factor.

Declarations

Acknowledgements

This review was supported by a training grant from the California Institute for Regenerative Medicine (T1-00007) and by NIH R01NS074831.

Authors’ Affiliations

(1)
Department of Chemical and Biomolecular Engineering, University of California Berkeley

References

  1. Schofield R: The relationship between the spleen colony-forming cell and the haemopoietic stem cell. Blood Cells. 1978, 4: 7-25.PubMed
  2. Xie T, Spradling AC: A niche maintaining germ line stem cells in the Drosophila ovary. Science. 2000, 290: 328-330. 10.1126/science.290.5490.328.View ArticlePubMed
  3. Ye S, Luo Y, Lu W, Jones RB, Linhardt RJ, Capila I, Toida T, Kan M, Pelletier H, McKeehan WL: Structural basis for interaction of FGF-1, FGF-2, and FGF-7 with different heparan sulfate motifs. Biochemistry. 2001, 40: 14429-14439. 10.1021/bi011000u.View ArticlePubMed
  4. Khachigian LM, Chesterman CN: Platelet-derived growth factor and alternative splicing: a review. Pathology. 1992, 24: 280-290. 10.3109/00313029209068882.View ArticlePubMed
  5. Hasan M, Najjam S, Gordon MY, Gibbs RV, Rider CC: IL-12 is a heparin-binding cytokine. J Immunol. 1999, 162: 1064-1070.PubMed
  6. Chang SC, Mulloy B, Magee AI, Couchman JR: Two distinct sites in sonic Hedgehog combine for heparan sulfate interactions and cell signaling functions. J Biol Chem. 2011, 286: 44391-44402. 10.1074/jbc.M111.285361.PubMed CentralView ArticlePubMed
  7. Tayalia P, Mooney DJ: Controlled growth factor delivery for tissue engineering. Adv Mater. 2009, 21: 3269-3285. 10.1002/adma.200900241.View ArticlePubMed
  8. Saha K, Schaffer DV: Signal dynamics in Sonic hedgehog tissue patterning. Development. 2006, 133: 889-900. 10.1242/dev.02254.View ArticlePubMed
  9. Chen TT, Luque A, Lee S, Anderson SM, Segura T, Iruela-Arispe ML: Anchorage of VEGF to the extracellular matrix conveys differential signaling responses to endothelial cells. J Cell Biol. 2010, 188: 595-609. 10.1083/jcb.200906044.PubMed CentralView ArticlePubMed
  10. Kuhl PR, Griffith-Cima LG: Tethered epidermal growth factor as a paradigm for growth factor-induced stimulation from the solid phase. Nat Med. 1996, 2: 1022-1027. 10.1038/nm0996-1022.View ArticlePubMed
  11. Ho JE, Chung EH, Wall S, Schaffer DV, Healy KE: Immobilized sonic hedgehog N-terminal signaling domain enhances differentiation of bone marrow-derived mesenchymal stem cells. J Biomed Mater Res A. 2007, 83: 1200-1208.View ArticlePubMed
  12. Willerth SM, Rader A, Sakiyama-Elbert SE: The effect of controlled growth factor delivery on embryonic stem cell differentiation inside fibrin scaffolds. Stem Cell Res. 2008, 1: 205-218. 10.1016/j.scr.2008.05.006.PubMed CentralView ArticlePubMed
  13. Alberti K, Davey RE, Onishi K, George S, Salchert K, Seib FP, Bornhauser M, Pompe T, Nagy A, Werner C, Zandstra PW: Functional immobilization of signaling proteins enables control of stem cell fate. Nat Methods. 2008, 5: 645-650. 10.1038/nmeth.1222.View ArticlePubMed
  14. Mammen M, Choi S-K, Whitesides GM: Polyvalent interactions in biological systems: implications for design and use of multivalent ligands and inhibitors. Angew Chem Int Ed. 1998, 37: 2754-2794. 10.1002/(SICI)1521-3773(19981102)37:20<2754::AID-ANIE2754>3.0.CO;2-3.View Article
  15. Kiessling LL, Gestwicki JE, Strong LE: Synthetic multivalent ligands in the exploration of cell-surface interactions. Curr Opin Chem Biol. 2000, 4: 696-703. 10.1016/S1367-5931(00)00153-8.View ArticlePubMed
  16. Gestwicki JE, Strong LE, Kiessling LL: Tuning chemotactic responses with synthetic multivalent ligands. Chem Biol. 2000, 7: 583-591. 10.1016/S1074-5521(00)00002-8.View ArticlePubMed
  17. Jones DS, Coutts SM, Gamino CA, Iverson GM, Linnik MD, Randow ME, Ton-Nu HT, Victoria EJ: Multivalent thioether-peptide conjugates: B cell tolerance of an anti-peptide immune response. Bioconjug Chem. 1999, 10: 480-488. 10.1021/bc9801373.View ArticlePubMed
  18. Maheshwari G, Brown G, Lauffenburger DA, Wells A, Griffith LG: Cell adhesion and motility depend on nanoscale RGD clustering. J Cell Sci. 2000, 113 (Pt 10): 1677-1686.PubMed
  19. Huebsch N, Arany PR, Mao AS, Shvartsman D, Ali OA, Bencherif SA, Rivera-Feliciano J, Mooney DJ: Harnessing traction-mediated manipulation of the cell/matrix interface to control stem-cell fate. Nat Mater. 2010, 9: 518-526. 10.1038/nmat2732.PubMed CentralView ArticlePubMed
  20. Vyas N, Goswami D, Manonmani A, Sharma P, Ranganath HA, VijayRaghavan K, Shashidhara LS, Sowdhamini R, Mayor S: Nanoscale organization of hedgehog is essential for long-range signaling. Cell. 2008, 133: 1214-1227. 10.1016/j.cell.2008.05.026.View ArticlePubMed
  21. Dore JJ, Edens M, Garamszegi N, Leof EB: Heteromeric and homomeric transforming growth factor-beta receptors show distinct signaling and endocytic responses in epithelial cells. J Biol Chem. 1998, 273: 31770-31777. 10.1074/jbc.273.48.31770.View ArticlePubMed
  22. Artavanis-Tsakonas S, Rand MD, Lake RJ: Notch signaling: cell fate control and signal integration in development. Science. 1999, 284: 770-776. 10.1126/science.284.5415.770.View ArticlePubMed
  23. Pasquale EB: Eph receptor signalling casts a wide net on cell behaviour. Nat Rev Mol Cell Biol. 2005, 6: 462-475. 10.1038/nrm1662.View ArticlePubMed
  24. Wall ST, Saha K, Ashton RS, Kam KR, Schaffer DV, Healy KE: Multivalency of Sonic hedgehog conjugated to linear polymer chains modulates protein potency. Bioconjug Chem. 2008, 19: 806-812. 10.1021/bc700265k.View ArticlePubMed
  25. Oh S, Brammer KS, Li YS, Teng D, Engler AJ, Chien S, Jin S: Stem cell fate dictated solely by altered nanotube dimension. Proc Natl Acad Sci USA. 2009, 106: 2130-2135. 10.1073/pnas.0813200106.PubMed CentralView ArticlePubMed
  26. Dalby MJ, Gadegaard N, Tare R, Andar A, Riehle MO, Herzyk P, Wilkinson CD, Oreffo RO: The control of human mesenchymal cell differentiation using nanoscale symmetry and disorder. Nat Mater. 2007, 6: 997-1003. 10.1038/nmat2013.View ArticlePubMed
  27. Kulangara K, Yang Y, Yang J, Leong KW: Nanotopography as modulator of human mesenchymal stem cell function. Biomaterials. 2012, 33: 4998-5003. 10.1016/j.biomaterials.2012.03.053.PubMed CentralView ArticlePubMed
  28. Ungrin MD, Joshi C, Nica A, Bauwens C, Zandstra PW: Reproducible, ultra high-throughput formation of multicellular organization from single cell suspension-derived human embryonic stem cell aggregates. PLoS One. 2008, 3: e1565-10.1371/journal.pone.0001565.PubMed CentralView ArticlePubMed
  29. Mohr JC, de Pablo JJ, Palecek SP: 3-D microwell culture of human embryonic stem cells. Biomaterials. 2006, 27: 6032-6042. 10.1016/j.biomaterials.2006.07.012.View ArticlePubMed
  30. Karp JM, Yeh J, Eng G, Fukuda J, Blumling J, Suh KY, Cheng J, Mahdavi A, Borenstein J, Langer R, Khademhosseini A: Controlling size, shape and homogeneity of embryoid bodies using poly(ethylene glycol) microwells. Lab Chip. 2007, 7: 786-794. 10.1039/b705085m.View ArticlePubMed
  31. Hwang YS, Chung BG, Ortmann D, Hattori N, Moeller HC, Khademhosseini A: Microwell-mediated control of embryoid body size regulates embryonic stem cell fate via differential expression of WNT5a and WNT11. Proc Natl Acad Sci USA. 2009, 106: 16978-16983. 10.1073/pnas.0905550106.PubMed CentralView ArticlePubMed
  32. Bauwens CL, Peerani R, Niebruegge S, Woodhouse KA, Kumacheva E, Husain M, Zandstra PW: Control of human embryonic stem cell colony and aggregate size heterogeneity influences differentiation trajectories. Stem Cells. 2008, 26: 2300-2310. 10.1634/stemcells.2008-0183.View ArticlePubMed
  33. Katzberg AA: Distance as a factor in the development of attraction fields between growing tissue in culture. Science. 1951, 114: 431-432. 10.1126/science.114.2965.431.View ArticlePubMed
  34. Weiss P, Garber B: Shape and movement of mesenchyme cells as functions of the physical structure of the medium: contributions to a quantitative morphology. Proc Natl Acad Sci USA. 1952, 38: 264-280. 10.1073/pnas.38.3.264.PubMed CentralView ArticlePubMed
  35. Warshaw DM, Mulvany MJ, Halpern W: Mechanical and morphological properties of arterial resistance vessels in young and old spontaneously hypertensive rats. Circ Res. 1979, 45: 250-259. 10.1161/01.RES.45.2.250.View ArticlePubMed
  36. Elkin BS, Azeloglu EU, Costa KD, Morrison B: Mechanical heterogeneity of the rat hippocampus measured by atomic force microscope indentation. J Neurotrauma. 2007, 24: 812-822. 10.1089/neu.2006.0169.View ArticlePubMed
  37. Engler AJ, Sen S, Sweeney HL, Discher DE: Matrix elasticity directs stem cell lineage specification. Cell. 2006, 126: 677-689. 10.1016/j.cell.2006.06.044.View ArticlePubMed
  38. Saha K, Keung AJ, Irwin EF, Li Y, Little L, Schaffer DV, Healy KE: Substrate modulus directs neural stem cell behavior. Biophys J. 2008, 95: 4426-4438. 10.1529/biophysj.108.132217.PubMed CentralView ArticlePubMed
  39. Keung AJ, Asuri P, Kumar S, Schaffer DV: Soft microenvironments promote the early neurogenic differentiation but not self-renewal of human pluripotent stem cells. Integr Biol (Camb). 2012, 4: 1049-1058. 10.1039/c2ib20083j.View Article
  40. Leight JL, Wozniak MA, Chen S, Lynch ML, Chen CS: Matrix rigidity regulates a switch between TGF-beta1-induced apoptosis and epithelial-mesenchymal transition. Mol Biol Cell. 2012, 23: 781-791. 10.1091/mbc.E11-06-0537.PubMed CentralView ArticlePubMed
  41. Tse JR, Engler AJ: Stiffness gradients mimicking in vivo tissue variation regulate mesenchymal stem cell fate. PLoS One. 2011, 6: e15978-10.1371/journal.pone.0015978.PubMed CentralView ArticlePubMed
  42. Winer JP, Oake S, Janmey PA: Non-linear elasticity of extracellular matrices enables contractile cells to communicate local position and orientation. PLoS One. 2009, 4: e6382-10.1371/journal.pone.0006382.PubMed CentralView ArticlePubMed
  43. Gilbert PM, Havenstrite KL, Magnusson KE, Sacco A, Leonardi NA, Kraft P, Nguyen NK, Thrun S, Lutolf MP, Blau HM: Substrate elasticity regulates skeletal muscle stem cell self-renewal in culture. Science. 2010, 329: 1078-1081. 10.1126/science.1191035.PubMed CentralView ArticlePubMed
  44. Chowdhury F, Li Y, Poh YC, Yokohama-Tamaki T, Wang N, Tanaka TS: Soft substrates promote homogeneous self-renewal of embryonic stem cells via downregulating cell-matrix tractions. PLoS One. 2010, 5: e15655-10.1371/journal.pone.0015655.PubMed CentralView ArticlePubMed
  45. Fu J, Wang YK, Yang MT, Desai RA, Yu X, Liu Z, Chen CS: Mechanical regulation of cell function with geometrically modulated elastomeric substrates. Nat Methods. 2010, 7: 733-736. 10.1038/nmeth.1487.PubMed CentralView ArticlePubMed
  46. Keung AJ, de Juan-Pardo EM, Schaffer DV, Kumar S: Rho GTPases mediate the mechanosensitive lineage commitment of neural stem cells. Stem Cells. 2011, 29: 1886-1897. 10.1002/stem.746.View ArticlePubMed
  47. Kim TJ, Seong J, Ouyang M, Sun J, Lu S, Hong JP, Wang N, Wang Y: Substrate rigidity regulates Ca2+ oscillation via RhoA pathway in stem cells. J Cell Physiol. 2009, 218: 285-293. 10.1002/jcp.21598.PubMed CentralView ArticlePubMed
  48. Dupont S, Morsut L, Aragona M, Enzo E, Giulitti S, Cordenonsi M, Zanconato F, Le Digabel J, Forcato M, Bicciato S, Elvassore N, Piccolo S: Role of YAP/TAZ in mechanotransduction. Nature. 2011, 474: 179-183. 10.1038/nature10137.View ArticlePubMed
  49. Trappmann B, Gautrot JE, Connelly JT, Strange DG, Li Y, Oyen ML, Cohen Stuart MA, Boehm H, Li B, Vogel V, Spatz JP, Watt FM, Huck WT: Extracellularmatrix tethering regulates stem-cell fate. Nat Mater. 2012, 11: 642-649. 10.1038/nmat3339.View ArticlePubMed
  50. Farge E: Mechanical induction of Twist in the Drosophila foregut/stomodeal primordium. Curr Biol. 2003, 13: 1365-1377. 10.1016/S0960-9822(03)00576-1.View ArticlePubMed
  51. Krieg M, Arboleda-Estudillo Y, Puech PH, Kafer J, Graner F, Muller DJ, Heisenberg CP: Tensile forces govern germ-layer organization in zebrafish. Nat Cell Biol. 2008, 10: 429-436. 10.1038/ncb1705.View ArticlePubMed
  52. Jakkaraju S, Zhe X, Pan D, Choudhury R, Schuger L: TIPs are tension-responsive proteins involved in myogenic versus adipogenic differentiation. Dev Cell. 2005, 9: 39-49. 10.1016/j.devcel.2005.04.015.View ArticlePubMed
  53. Saha S, Ji L, de Pablo JJ, Palecek SP: TGFbeta/Activin/Nodal pathway in inhibition of human embryonic stem cell differentiation by mechanical strain. Biophys J. 2008, 94: 4123-4133. 10.1529/biophysj.107.119891.PubMed CentralView ArticlePubMed
  54. Park JS, Chu JS, Cheng C, Chen F, Chen D, Li S: Differential effects of equiaxial and uniaxial strain on mesenchymal stem cells. Biotechnol Bioeng. 2004, 88: 359-368. 10.1002/bit.20250.View ArticlePubMed
  55. Gross J, Lapiere CM: Collagenolytic activity in amphibian tissues: a tissue culture assay. Proc Natl Acad Sci USA. 1962, 48: 1014-1022. 10.1073/pnas.48.6.1014.PubMed CentralView ArticlePubMed
  56. Barkho BZ, Munoz AE, Li X, Li L, Cunningham LA, Zhao X: Endogenous matrix metalloproteinase (MMP)-3 and MMP-9 promote the differentiation and migration of adult neural progenitor cells in response to chemokines. Stem Cells. 2008, 26: 3139-3149. 10.1634/stemcells.2008-0519.PubMed CentralView ArticlePubMed
  57. Lu C, Li XY, Hu Y, Rowe RG, Weiss SJ: MT1-MMP controls human mesenchymal stem cell trafficking and differentiation. Blood. 2010, 115: 221-229. 10.1182/blood-2009-06-228494.PubMed CentralView ArticlePubMed
  58. Young JL, Engler AJ: Hydrogels with time-dependent material properties enhance cardiomyocyte differentiation in vitro. Biomaterials. 2011, 32: 1002-1009. 10.1016/j.biomaterials.2010.10.020.PubMed CentralView ArticlePubMed
  59. Guvendiren M, Burdick JA: Stiffening hydrogels to probe short- and long-term cellular responses to dynamic mechanics. Nat Commun. 2012, 3: 792-View ArticlePubMed
  60. Kloxin AM, Benton JA, Anseth KS: In situ elasticity modulation with dynamic substrates to direct cell phenotype. Biomaterials. 2010, 31: 1-8. 10.1016/j.biomaterials.2009.09.025.PubMed CentralView ArticlePubMed
  61. Yamamoto K, Takahashi T, Asahara T, Ohura N, Sokabe T, Kamiya A, Ando J: Proliferation, differentiation, and tube formation by endothelial progenitor cells in response to shear stress. J Appl Physiol. 2003, 95: 2081-2088.View ArticlePubMed
  62. Wang H, Riha GM, Yan S, Li M, Chai H, Yang H, Yao Q, Chen C: Shear stress induces endothelial differentiation from a murine embryonic mesenchymal progenitor cell line. Arterioscler Thromb Vasc Biol. 2005, 25: 1817-1823. 10.1161/01.ATV.0000175840.90510.a8.View ArticlePubMed
  63. Illi B, Scopece A, Nanni S, Farsetti A, Morgante L, Biglioli P, Capogrossi MC, Gaetano C: Epigenetic histone modification and cardiovascular lineage programming in mouse embryonic stem cells exposed to laminar shear stress. Circ Res. 2005, 96: 501-508. 10.1161/01.RES.0000159181.06379.63.View ArticlePubMed
  64. Yamamoto K, Sokabe T, Watabe T, Miyazono K, Yamashita JK, Obi S, Ohura N, Matsushita A, Kamiya A, Ando J: Fluid shear stress induces differentiation of Flk-1-positive embryonic stem cells into vascular endothelial cells in vitro. Am J Physiol Heart Circ Physiol. 2005, 288: H1915-1924.View ArticlePubMed
  65. Radisic M, Park H, Shing H, Consi T, Schoen FJ, Langer R, Freed LE, Vunjak-Novakovic G: Functional assembly of engineered myocardium by electrical stimulation of cardiac myocytes cultured on scaffolds. Proc Natl Acad Sci USA. 2004, 101: 18129-18134. 10.1073/pnas.0407817101.PubMed CentralView ArticlePubMed
  66. Carpenedo RL, Bratt-Leal AM, Marklein RA, Seaman SA, Bowen NJ, McDonald JF, McDevitt TC: Homogeneous and organized differentiation within embryoid bodies induced by microsphere-mediated delivery of small molecules. Biomaterials. 2009, 30: 2507-2515. 10.1016/j.biomaterials.2009.01.007.PubMed CentralView ArticlePubMed
  67. Shroff H, Galbraith CG, Galbraith JA, Betzig E: Live-cell photoactivated localization microscopy of nanoscale adhesion dynamics. Nat Methods. 2008, 5: 417-423. 10.1038/nmeth.1202.View ArticlePubMed

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