Ixmyelocel-T, an expanded multicellular therapy, contains a unique population of M2-like macrophages
© Ledford et al.; licensee BioMed Central Ltd. 2013
Received: 19 March 2013
Accepted: 23 October 2013
Published: 1 November 2013
M2 macrophages promote tissue repair and regeneration through various mechanisms including immunomodulation and scavenging of tissue debris. Delivering increased numbers of these cells to ischemic tissues may limit tissue injury and promote repair. Ixmyelocel-T is an expanded, autologous multicellular therapy cultured from bone-marrow mononuclear cells (BMMNCs). The purpose of this study was to characterize further a unique expanded population of M2-like macrophages, generated in ixmyelocel-T therapy.
Approximately 50 ml of whole bone marrow was obtained from healthy donors and shipped overnight. BMMNCs were produced by using density-gradient separation and cultured for approximately 12 days to generate ixmyelocel-T. CD14+ cells were isolated from ixmyelocel-T with positive selection for analysis. Cell-surface phenotype was examined with flow cytometry and immunofluorescence, and expression of cytokines and chemokines was analyzed with enzyme-linked immunosorbent assay (ELISA). Quantitative real-time PCR was used to analyze expression of genes in BMMNCs, ixmyelocel-T, the CD14+ population from ixmyelocel-T, and M1 and M2 macrophages. Ixmyelocel-T was cultured with apoptotic BMMNCs, and then visualized under fluorescence microscopy to assess efferocytosis.
Macrophages in ixmyelocel-T therapy expressed surface markers of M2 macrophages, CD206, and CD163. These cells were also found to express several M2 markers, and few to no M1 markers. After stimulation with lipopolysaccharide (LPS), they showed minimal secretion of the proinflammatory cytokines interleukin-12 (IL-12) and tumor necrosis factor alpha (TNF-α) compared with M1 and M2 macrophages. Ixmyelocel-T macrophages efficiently ingested apoptotic BMMNCs.
Ixmyelocel-T therapy contains a unique population of M2-like macrophages that are characterized by expression of M2 markers, decreased secretion of proinflammatory cytokines after inflammatory stimuli, and efficient removal of apoptotic cells. This subpopulation of cells may have a potential role in tissue repair and regeneration.
Macrophages are a diverse population of cells that adapt and respond to a variety of signals, including cytokines and microbial products . Macrophages can be classified based on their functional phenotypes; M1 macrophages are classically activated by proinflammatory cytokines such as gamma interferon (IFN-ɣ) and are T-helper 1 (Th1) associated, whereas M2 macrophages are alternatively activated by cytokines such as IL-4 and IL-13, and are T-helper 2 (Th2) associated . Both in vitro and in vivo studies have demonstrated that M1 macrophages have an inflammatory phenotype that corresponds with the early phases of tissue injury , whereas M2 macrophages have an antiinflammatory and tissue-remodeling phenotype corresponding with the late phases of tissue injury [1, 3–8]. M2 macrophages help promote clearance of inflammatory cells and the return of tissue homeostasis . Recently, it has become apparent that these macrophage classifications are extremes of a wide spectrum of possible macrophage phenotypes [8–10].
Several diseases are associated with a defect or alteration in macrophage function [5, 6, 11]. M2 macrophages are characterized as immunosuppressive and reparative, and have been implicated in stable areas of atherosclerotic lesions, myocardial infarction healing, and skeletal muscle repair [12, 13]. Several studies have demonstrated that macrophages are differentially activated during cardiac remodeling after myocardial infarction, with M2 macrophages being involved in the reparative phase [11, 12, 14]. Studies have also shown that atherosclerotic lesions are characterized by the presence of proinflammatory M1 macrophages that fail to switch to an antiinflammatory and reparative phenotype, thus promoting disease progression [5, 15–17]. Therefore, increasing the proportion of M2 macrophages in such disease states could be used to limit tissue injury and promote repair.
Ixmyelocel-T is an expanded, autologous multicellular therapy containing a mixture of cell types cultured from BMMNCs [18–20]. Recent clinical trials evaluating ixmyelocel-T therapy in the treatment of dilated cardiomyopathy and severe peripheral artery disease have shown clinical promise [19, 21, 22]. Ixmyelocel-T contains a mixture of cells; the process used to generate this cell therapy expands both the CD90+ mesenchymal stromal cells (MSCs) and CD14+ macrophages, while retaining many of the CD45+ cells found in the bone marrow, because the process does not use any purification or enrichment steps, other than phenotypic expansion. The MSCs have been previously characterized both in vitro and in vivo [18, 20]. Although early development of ixmyelocel-T was focused on bone-regeneration studies due to the more obvious expansion of the MSCs which were extremely rare in bone marrow, an initial characterization of the CD14+ hematopoietic cells , demonstrated the simultaneous expansion of macrophages, which were primarily responsible for the secretion of high levels of IL-10 and IL-1a receptor antagonist, and were therefore consistent with these macrophages being M2-like.
The purpose of this study was to further characterize ixmyelocel-T macrophages, and determine more precisely their phenotype. The population of macrophages generated in ixmyelocel-T, may be beneficial in the treatment of diseases where tissue remodeling and immunomodulation are key components of successful clinical outcomes. Ixmyelocel-T macrophages are characterized as M2-like with minimal secretion of pro-inflammatory cytokines after inflammatory challenge, as well as efficient removal of apoptotic cells (efferocytosis). Additionally, this data provides evidence that ixmyelocel-T contains a unique M2-like population of macrophages when compared to in vitro generated M1 and M2 macrophages.
For the generation of ixmyelocel-T commercially available bone marrow aspirates (Lonza, MD, USA) were obtained from healthy donors under informed consent. A small volume (~50 mL) of whole bone marrow was obtained through needle aspiration of the posterior iliac crest, and stored in heparinized tubes during shipment at ambient temperature to a central processing facility. The mononuclear cell fraction was obtained via an automated, closed-system, Ficoll-based density gradient centrifugation separation process. The isolated mononuclear cells were then transferred to a sterile, single-use cell bioreactor cassette [20, 23]. This proprietary system controlled temperature, culture medium exchange, and gas exchange during the culture period. After approximately 12 days, the cells were washed and harvested from the cassette by a multistep, automated process, and ready for experimental study. For the generation of M1 and M2 macrophages, human peripheral blood monocytes from healthy donors were purchased (AllCells LLC., Alameda, USA). The cells were polarized into M1 and M2 macrophages using established protocols [24, 25]. Briefly, monocytes were differentiated into macrophages by incubation with 100 ng/mL M-CSF (R&D Systems, Minneapolis, MN, USA) in RPMI-1640 (Invitrogen) supplemented with 20% FBS (Invitrogen) for 7 days. The media was then removed and the macrophages were then polarized with 10 pg/mL LPS plus 20 ng/mL interferon gamma (IFN-ɣ) for M1 polarization, or 20 ng/mL IL-4 for M2 polarization (R&D Systems, Minneapolis, MN, USA) in RPMI-1640 with 5% FBS for 18 hours.
CD14+ cell purification
CD14+ cells were isolated from ixmyelocel-T by positive selection using MACS beads (Miltenyi Biotec, Bergisch Gladbach, Germany) as per the manufacturer’s instructions. After positive selection, the CD14+ ixmyelocel-T macrophages were transferred to 6-well culture plates for subsequent experiments. Re-analyses of the selected cells by flow cytometry indicated a relatively pure preparation (approximately >90%).
For cell surface staining, erythrocytes were lysed with lysing solution for 10 minutes (Becton Dickinson, Sad Jose, CA). Fc receptors were blocked with Fc receptor blocking agent (Miltenyi Biotech, Auburn, CA, USA) for 15 minutes at 4°C. Cells were then incubated with surface receptor antibodies for 15 minutes at 4°C, and then washed with phosphate buffered saline (PBS). Cell surface staining was analyzed using the Gallios flow cytometer (Beckman Coulter, Brea, CA, USA). Kaluza software (Beckman Coulter) was used to analyze the acquired data. The following antibodies were used for the analyses: anti-CD14 FITC, anti-CD14 APC, anti-CD206 PE, anti-CD16 ECD, anti-HLA-DR ECD and Annexin V FITC (Beckman Coulter), anti-CD163 APC and anti-CD163 PerCP5.5 (BioLegend, San Diego, CA, USA), and anti-MerTK (R&D Systems).
To visualize alternatively activated macrophages in ixmyelocel-T, cells were seeded in chamber slides and immunostained with CD14, CD90, CD3 antibody (Santa Cruz Biotechnology Inc) for 1 hour at room temperature prior to incubation with fluorochrome-tagged secondary antibody for 1 hour at room temperature. Counterstaining was performed with DAPI to visualize nuclei. Fluorescent microscopy was performed using a Nikon Eclipse TE2000-S Microscope (Nikon, Tokyo, Japan) equipped with a Spot Xplorer Leica digital camera (SPOT, Sterling Heights, MI, USA).
Real-time quantitative PCR
For real-time PCR analysis, CD14+ selected macrophages from ixmyelocel-T were compared to CD14+ monocytes from the starting BMMNC population, M1 Macrophages, or M2 Macrophages. Total RNA was extracted with the RNeasy Mini Kit (Qiagen, Valencia, CA, USA) and 1 μg of RNA was reverse transcribed using a high capacity cDNA reverse transcription kit per the manufacturer’s directions (Applied Biosciences, Carlsbad, CA, USA). Relative levels of target gene expression were measured on the 7500 Real-Time PCR system (Applied Biosystems). FAM-based Taqman Gene Expression Assay Mix (Applied Biosystems) specific for each gene of interest and Taqman Universal Master Mix (Applied Biosystems) were used. Relative quantification PCR analysis was performed using the ABI 7500 Software (Applied Biosystems). The relative amount of cDNA was calculated by normalization to the relative levels of Gapdh.
Enzyme-linked immunosorbent assay (ELISA) kits were used to determine the concentrations of the following cytokines: interleukin (IL)-10, IL-1ra, tumor necrosis factor alpha (TNFα), and IL-12 p70 (R&D Systems, Minneapolis, MN, USA). For cytokine analysis of ixmyelocel-T vs. BMMNCs, cells were plated in the presence or absence of 0.1 μg/mL lipopolysaccharide (LPS) overnight. After LPS challenge, supernatants were collected and assayed for cytokines. For cytokine analysis of ixmyelocel-T macrophages vs. M1/M2 macrophages, cells were positively selected using CD14 MACS beads (Miltenyi Biotech) to isolate macrophages. After selection, cells were plated in the presence or absence of 0.1 μg/mL lipopolysaccharide (LPS) overnight. After LPS challenge, supernatants were collected and assayed for cytokines.
BMMNCs were cryopreserved for use in the efferocytosis experiments. Frozen BMMNCs were thawed, washed, and then labeled with the lipophilic dye PKH26 as recommended by the manufacturer (Sigma, St. Louis, MO, USA), and apoptosis was induced with 2 μM staurosporine (Sigma). The apoptotic PKH26-labeled BMMNCs were added in a 1:1 concentration to ixmyelocel-T derived from the same marrow donor. For fluorescent microscopy, ixmyelocel-T was labeled with PKH67 as recommended by the manufacturer (Sigma). Ixmyelocel-T was incubated with the apoptotic PKH26-labeled BMMNCs for 3 hours and then washed with PBS. The ixmyelocel-T samples mixed with apoptotic cells were then analyzed by fluorescent microscopy to determine if ixmyelocel-T macrophages ingested apoptotic cells.
Paired t-test was performed to compare results. A p-value less than 0.05 was considered statistically significant. Data are reported as mean ± SEM.
Ixmyelocel-T contains a population of M2-like macrophages
Ixmyelocel-T macrophages are a unique M2-like macrophage
Ixmyelocel-T macrophages express MerTK, a receptor involved in efferocytosis
In this report, ixmyelocel-T was found to secrete significantly elevated levels of both IL-10 and IL-1ra before and after LPS stimulation, compared to BMMNCs. Additionally, both ixmyelocel-T and BMMNCs were found to secrete minimal amounts of the pro-inflammatory cytokines TNFα and IL-12. After LPS challenge both ixmyelocel-T and BMMNCs secreted significantly more TNFα, however this increase in secretion was significantly higher in BMMNCs. This expanded population of M2-like macrophages was also found to express significantly elevated levels of the M2 markers CD206, CD163, SR-B1, MerTK, PPARɣ, and TGF-β. Together these findings suggest that the 12 ± 1 day ex vivo expansion of autologous marrow generates a population of M2-like macrophages in ixmyelocel-T.
Ixmyelocel-T macrophages were demonstrated to express 2 well-characterized surface markers of M2 macrophages, CD206 and CD163. Whereas surface expression of markers of pro-inflammatory macrophages, CD16 and HLA-DR [36, 37], were found to be barely expressed. Studies have demonstrated that macrophages with high expression of CD16 efficiently produce pro-inflammatory cytokines including TNFα and IL-12, while no or very little anti-inflammatory cytokines, whereas macrophages with low expression of CD16 produce low levels of pro-inflammatory cytokines and secrete elevated levels of anti-inflammatory cytokines such as IL-10 [37, 38]. These findings suggest that ixmyelocel-T consists mainly of macrophages with anti-inflammatory M2-like phenotypes.
In vivo, bone marrow-derived macrophages differentiate from circulating peripheral blood monocytes after migration into tissues, often in response to injury or insult. These macrophages can then be polarized into M1 or M2 macrophages by their microenvironment . As a cellular therapy, ixmyelocel-T macrophages are generated ex vivo from bone marrow and directly injected into areas where repair is needed [20, 22]. In order to further explore the differences between these cell phenotypes ixmyelocel-T macrophages were compared to M2 and M1 macrophages. Both Ixmyelocel-T and M2 macrophages were found to express similar levels of PPARɣ. Ixmyelocel-T macrophages were found to express significantly lower levels of the scavenger receptors CD206, CD204, and SR-B1 compared to M2 macrophages. However, ixmyelocel-T macrophages were found to express significantly higher expression of the scavenger receptors CD163 and MerTK compared to M2 macrophages. Ixmyelocel-T macrophages were also found to have significantly higher expression of TGF-β compared to M1 and M2 macrophages. When compared to M1 macrophages both M2 and ixmyelocel-T macrophages were found to express significantly lower levels of the M1 markers CCR7, TNFα, and IL-1β. Together these data suggest that ixmyelocel-T macrophages are more M2-like. This data also suggests that ixmyelocel-T macrophages may represent a unique M2-like macrophage, with slightly different expression of M2 markers. Future studies will further examine the unique M2- like phenotype of ixmyelocel-T macrophages to determine just how similar or different they are to M2 macrophages.
Macrophages are plastic cells that can switch from an activated M1 state back to M2, and vice versa depending on specific signals [26, 33]. Previous reports have demonstrated that M2 macrophages can be polarized back to a M1 state using pro-inflammatory stimuli such as LPS . To determine if ixmyelocel-T macrophages could also be polarized back to a M1 state in a similar fashion M1, M2, and ixmyelocel-T macrophages were treated with LPS overnight. Before LPS stimulation both M2 and ixmyelocel-T macrophages secreted significantly less TNFα and IL-12 p70 compared to M1 macrophages. However after overnight LPS stimulation, M2 macrophages secreted significantly elevated levels of TNFα and IL-12 p70 similar to M1 levels. This same amount of pro-inflammatory cytokine secretion was not mimicked by ixmyelocel-T macrophages. While ixmyelocel-T macrophages did secrete significantly elevated levels of TNFα after LPS stimulation, this amount of TNFα was significantly lower than that secreted by M2 macrophages. Additionally, ixmyelocel-T macrophages secreted significantly less IL-12 p70 after LPS stimulation compared to M1 and M2 macrophages. These data suggest that ixmyelocel-T macrophages are not readily polarized to a pro-inflammatory phenotype, suggesting that these cells might be beneficial in the treatment of inflammatory diseases. Future studies will further investigate this unique property of ixmyelocel-T macrophages to determine if they can affect the inflammatory state in vivo.
M2 macrophages are potent phagocytes that favorably bind and ingest early apoptotic cells . Apoptotic cells need to be removed quickly to prevent the release of tissue-damaging intracellular components that can induce inflammatory responses [28, 41]. Efferocytosis itself also triggers pro-resolving signals that promote dampening of the immune response and restoring tissue homeostasis . Defective clearance of apoptotic cells, or efferocytosis, is linked to the progression of several disease states including advanced atherosclerotic lesions, ischemic heart disease, and chronic wounds . Ixmyelocel-T macrophages were found to readily ingest apoptotic cells and express genes for the scavenger receptors that promote the phagocytosis of apoptotic cells. Of specific interest, ixmyelocel-T expresses the scavenger receptor MerTK, which has been implicated in mediating the anti-inflammatory clearance of apoptotic cells [29, 42]. The expression of scavenger receptors and their M2-like phenotype predisposes ixmyelocel-T macrophages to be potent phagocytes. This important function of ixmyelocel-T macrophages could potentially promote healing in disease states where efferocytosis is compromised. Specifically, advanced atherosclerotic lesions are characterized by defective efferocytosis, resulting in necrotic core formation [28, 42]. In early atherosclerotic lesions, apoptotic cells are rapidly removed by macrophages, which prevents the progression of lesions, but as the disease progresses, apoptotic cells are not removed, resulting in a pro-inflammatory cascade . Efferocytosis is also required in healing the heart after ischemia where removing necrotic debris helps preserve the remaining cardiomyocytes . As such, the macrophages present in ixmyelocel-T may potentially promote tissue repair and regeneration through the removal of necrotic debris.
Several different mechanisms have been reported in the literature that may contribute to the development of M2 macrophages, including ingestion of apoptotic cells, exposure to the Th2 cytokines IL-4 and IL-13, exposure to IL-10, and contact with mesenchymal stem cells [13, 27, 39, 43]. The culture process used to generate ixmyelocel-T results in a population of unique M2-like macrophages. Th2 cytokines are not directly added to ixmyelocel-T during the culture process to produce M2 macrophages [1, 31, 44]. However, due to the wide mixture of cells found within ixmyelocel-T it is feasible that Th2 cytokines may be generated and secreted by other cells during this 12 ± 1 day process. Ixmyelocel-T is composed of a mixture of cells including macrophages, granulocytes, monocytes, T cells, B cells, and MSC/stromal cells, and cytokine secretion from these different cell types could influence the development of the M2-like phenotype . For example, it has been previously reported that macrophages engage in bidirectional interactions with MSCs, resulting in MSC growth and M2 phenotype polarization [7, 12, 45]. MSCs have been reported to induce an IL-10 high and IL-12 low M2 phenotype in macrophages . MSCs are expanded during the ixmyelocel-T culture process; the interaction of these cells with macrophages in culture could contribute to the M2-like population of cells found within ixmyelocel-T. Future studies will further examine the relationship between these different cell types to determine if they play a role in the development of this M2-like population of macrophages.
Ixmyelocel-T is currently being evaluated in a Phase 2b clinical trial of advanced heart failure. Tissue recovery after injury is a complex process involving an interplay between macrophages, stem cells, and stromal cells to prevent tissue fibrosis, which can lead to ineffective tissue function . Recent work has highlighted the role of M2 macrophages in these processes [7, 17, 46]. Macrophages are commonly found in association with areas of fibrosis in cardiac tissue in end-stage heart disease , and recent studies have provided evidence that M2-like macrophages have the capacity to remove tissue debris and dampen inflammation in cardiac tissue promoting tissue repair . Additionally, recent studies have found that macrophages found in human carotid artery atherosclerotic plaques are dominated by a M1 phenotype , and that the number of pro-inflammatory M1 macrophages found in cardiac adipose tissue correlates with the severity of coronary artery atherosclerosis in human . Therefore, ixmyelocel-T may potentially promote tissue repair and regeneration in these disease states by providing a M2-like population of macrophages described herein. The biological properties of these macrophages in ixmyelocel-T may potentially have clinical utility for tissue repair and regeneration in the disease states where a population of M2 macrophages would be critical.
This study further examined the expanded population of macrophages found in ixmyelocel-T, since macrophages have been reported to play specific and unique roles in tissue regeneration and repair [7, 17, 46]. Several cellular therapies are currently being explored for the treatment of ischemic cardiovascular diseases where tissue remodeling and immunomodulation are considered key components of successful clinical outcomes. BMMNCs, peripheral blood mononuclear cells, endothelial progenitor cells (EPC), and MSCs are all currently being evaluated in the treatment of ischemic cardiovascular diseases . Tissue regeneration is a complex process involving an interplay between macrophages, stem cells, and stromal cells to prevent tissue fibrosis, which can lead to ineffective tissue function, and it is hypothesized that a mixture of regenerative cells, rather than just a single cell type might be more advantageous [7, 20, 48, 49]. Ixmyelocel-T consists of a mixture of cells generated from BMMNCs, specifically an expanded population of CD90+ stromal cells and CD14+ macrophages which have been characterized with a M2-like phenotype . It is thought that the mixture of cells found in ixmyelocel-T might be more advantageous in long-term tissue regeneration and repair [20, 48]. Future studies will further characterize the other cells types that make up ixmyelocel-T in order to highlight the potential roles these other subpopulations might play in tissue repair and regeneration.
There are several limitations to this study. Mainly, the findings and conclusions are based on in vitro experiments. The outcome of infiltrating macrophages to areas of inflammatory injury is not fully understood as the inflammatory environment may influence the outcome of the cells. Although we used human cells that would require the use of immune-compromised animals, in vivo experiments would strengthen the findings. Additionally, the cells in these experiments were obtained from healthy donors. It would be highly interesting to compare ixmyelocel-T macrophages to the peripheral blood macrophages (polarized M1 and M2) from a diseased patient. Studies have reported that several disease states, such as obesity and type 2 diabetes, affect macrophage phenotype and polarization in some cases limiting M2 polarization capacity [26, 50]. Therefore, it would useful to compare ixmyelocel-T macrophages to peripheral blood macrophages in clinically relevant states; especially disease states where ixmyelocel-T is injected as a treatment.
Our data demonstrate that ixmyelocel-T therapy contains a unique population of M2-like macrophages that are characterized by secretion of anti-inflammatory cytokines and expression of M2 markers- CD206 and CD163. Furthermore, these cells are involved in efficient removal of apoptotic cells and have elevated expression of MerTK, which is imperative in limiting tissue injury and promoting repair. The biological properties of the M2-like macrophages in ixmyelocel-T may have clinical utility for tissue repair and regeneration in the disease states where a population of M2 macrophages would be critical.
Bone marrow blood mononuclear cell
Enzyme-linked immunosorbent assay
Endothelial progenitor cell
Glyceraldehyde 3-phosphate dehydrogenase
Mesenchymal stromal cells
Phosphate buffered saline
Polymerase chain reaction
Peroxisome proliferator-activated receptor gamma
Transforming growth factor
T helper 1
T helper 2
Tumor necrosis factor alpha.
The authors would like to thank Alden Wong, Judith Schmitt, Hillary Evens, John Osborne, and Nikki Murphy for excellent technical assistance and Chis Parrish for his expertise with flow cytometry. Scientific editorial support was sponsored by Aastrom Biosciences, Inc, and provided by David E. Kaminsky, PhD, of AlphaBioCom, LLC.
- Bouhlel MA, Derudas B, Rigamonti E, Dievart R, Brozek J, Haulon S, Zawadzki C, Jude B, Torpier G, Marx N, Staels B, Chinetti-Gbaguidi G: PPARgamma activation primes human monocytes into alternative M2 macrophages with anti-inflammatory properties. Cell Metab. 2007, 6: 137-143. 10.1016/j.cmet.2007.06.010.View ArticlePubMedGoogle Scholar
- Mantovani A, Sica A, Locati M: New vistas on macrophage differentiation and activation. Eur J Immunol. 2007, 37: 14-16. 10.1002/eji.200636910.View ArticlePubMedGoogle Scholar
- Jennewein C, Kuhn AM, Schmidt MV, Meilladec-Jullig V, von Knethen A, Gonzalez FJ, Brune B: Sumoylation of peroxisome proliferator-activated receptor gamma by apoptotic cells prevents lipopolysaccharide-induced NCoR removal from kappaB binding sites mediating transrepression of proinflammatory cytokines. J Immunol. 2008, 181: 5646-5652.PubMed CentralView ArticlePubMedGoogle Scholar
- Mosser DM: The many faces of macrophage activation. J Leukoc Biol. 2003, 73: 209-212. 10.1189/jlb.0602325.View ArticlePubMedGoogle Scholar
- Deonarine K, Panelli MC, Stashower ME, Jin P, Smith K, Slade HB, Norwood C, Wang E, Marincola FM, Stroncek DF: Gene expression profiling of cutaneous wound healing. J Transl Med. 2007, 5: 11-10.1186/1479-5876-5-11.PubMed CentralView ArticlePubMedGoogle Scholar
- Mantovani A, Garlanda C, Locati M: Macrophage diversity and polarization in atherosclerosis: a question of balance. Arterioscler Thromb Vasc Biol. 2009, 29: 1419-1423. 10.1161/ATVBAHA.108.180497.View ArticlePubMedGoogle Scholar
- Ortega-Gomez A, Perretti M, Soehnlein O: Resolution of inflammation: an integrated view. EMBO Mol Med. 2013, 5: 661-674. 10.1002/emmm.201202382.PubMed CentralView ArticlePubMedGoogle Scholar
- Pinto AR, Paolicelli R, Salimova E, Gospocic J, Slonimsky E, Bilbao-Cortes D, Godwin JW, Rosenthal NA: An abundant tissue macrophage population in the adult murine heart with a distinct alternatively-activated macrophage profile. PLoS One. 2012, 7: e36814-10.1371/journal.pone.0036814.PubMed CentralView ArticlePubMedGoogle Scholar
- Meznarich J, Malchodi L, Helterline D, Ramsey SA, Bertko K, Plummer T, Plawman A, Gold E, Stempien-Otero A: Urokinase plasminogen activator induces pro-fibrotic/m2 phenotype in murine cardiac macrophages. PLoS One. 2013, 8: e57837-10.1371/journal.pone.0057837.PubMed CentralView ArticlePubMedGoogle Scholar
- Khallou-Laschet J, Varthaman A, Fornasa G, Compain C, Gaston AT, Clement M, Dussiot M, Levillain O, Graff-Dubois S, Nicoletti A, Caligiuri G: Macrophage plasticity in experimental atherosclerosis. PLoS One. 2010, 5: e8852-10.1371/journal.pone.0008852.PubMed CentralView ArticlePubMedGoogle Scholar
- Frangogiannis NG: Regulation of the inflammatory response in cardiac repair. Circ Res. 2012, 110: 159-173. 10.1161/CIRCRESAHA.111.243162.PubMed CentralView ArticlePubMedGoogle Scholar
- Adutler-Lieber S, Ben-Mordechai T, Naftali-Shani N, Asher E, Loberman D, Raanani E, Leor J: Human macrophage regulation via interaction with cardiac adipose tissue-derived mesenchymal stromal cells. J Cardiovasc Pharmacol Ther. 2013, 18: 78-86. 10.1177/1074248412453875.View ArticlePubMedGoogle Scholar
- Kharraz Y, Guerra J, Mann CJ, Serrano AL, Munoz-Canoves P: Macrophage plasticity and the role of inflammation in skeletal muscle repair. Mediators Inflamm. 2013, 2013: 491497-PubMed CentralView ArticlePubMedGoogle Scholar
- Thorp EB: Contrasting Inflammation Resolution during Atherosclerosis and Post Myocardial Infarction at the Level of Monocyte/Macrophage Phagocytic Clearance. Front Immunol. 2012, 31: 1-8.Google Scholar
- Khanna S, Biswas S, Shang Y, Collard E, Azad A, Kauh C, Bhasker V, Gordillo GM, Sen CK, Roy S: Macrophage dysfunction impairs resolution of inflammation in the wounds of diabetic mice. PLoS One. 2010, 5: e9539-10.1371/journal.pone.0009539.PubMed CentralView ArticlePubMedGoogle Scholar
- Waldo SW, Li Y, Buono C, Zhao B, Billings EM, Chang J, Kruth HS: Heterogeneity of human macrophages in culture and in atherosclerotic plaques. Am J Pathol. 2008, 172: 1112-1126. 10.2353/ajpath.2008.070513.PubMed CentralView ArticlePubMedGoogle Scholar
- Sindrilaru A, Peters T, Wieschalka S, Baican C, Baican A, Peter H, Hainzl A, Schatz S, Qi Y, Schlecht A, Weiss JM, Wlaschek M, Sunderkotter C, Scharffetter-Kochanek K: An unrestrained proinflammatory M1 macrophage population induced by iron impairs wound healing in humans and mice. J Clin Invest. 2011, 121: 985-997. 10.1172/JCI44490.PubMed CentralView ArticlePubMedGoogle Scholar
- Yin D, Wang Z, Gao Q, Sundaresan R, Parrish C, Yang Q, Krebsbach PH, Lichtler AC, Rowe DW, Hock J, Liu P: Determination of the fate and contribution of ex vivo expanded human bone marrow stem and progenitor cells for bone formation by 2.3ColGFP. Mol Ther. 2009, 17: 1967-1978. 10.1038/mt.2009.151.PubMed CentralView ArticlePubMedGoogle Scholar
- Powell RJ, Comerota AJ, Berceli SA, Guzman R, Henry TD, Tzeng E, Velazquez O, Marston WA, Bartel RL, Longcore A, Stern T, Watling S: Interim analysis results from the RESTORE-CLI, a randomized, double-blind multicenter phase II trial comparing expanded autologous bone marrow-derived tissue repair cells and placebo in patients with critical limb ischemia. J Vasc Surg. 2011Google Scholar
- Bartel RL, Cramer C, Ledford K, Longcore A, Parrish C, Stern T, Watling S, Zeigler F: The Aastrom experience. Stem Cell Res Ther. 2012, 3: 26-10.1186/scrt117.PubMed CentralView ArticlePubMedGoogle Scholar
- Comerota AJ, Link A, Douville J, Burchardt ER: Upper extremity ischemia treated with tissue repair cells from adult bone marrow. J Vasc Surg. 2010, 52: 723-729. 10.1016/j.jvs.2010.04.020.View ArticlePubMedGoogle Scholar
- Powell RJ, Marston WA, Berceli SA, Guzman R, Henry TD, Longcore AT, Stern TP, Watling S, Bartel RL: Cellular therapy with Ixmyelocel-T to treat critical limb ischemia: the randomized, double-blind, placebo-controlled RESTORE-CLI trial. Mol Ther. 2012, 20: 1280-1286. 10.1038/mt.2012.52.PubMed CentralView ArticlePubMedGoogle Scholar
- Gastens MH, Goltry K, Prohaska W, Tschope D, Stratmann B, Lammers D, Kirana S, Gotting C, Kleesiek K: Good manufacturing practice-compliant expansion of marrow-derived stem and progenitor cells for cell therapy. Cell Transplant. 2007, 16: 685-696.View ArticlePubMedGoogle Scholar
- Martinez FO, Gordon S, Locati M, Mantovani A: Transcriptional profiling of the human monocyte-to-macrophage differentiation and polarization: new molecules and patterns of gene expression. J Immunol. 2006, 177: 7303-7311.View ArticlePubMedGoogle Scholar
- Hirose K, Iwabuchi K, Shimada K, Kiyanagi T, Iwahara C, Nakayama H, Daida H: Different responses to oxidized low-density lipoproteins in human polarized macrophages. Lipids Health Dis. 2011, 10: 1-10.1186/1476-511X-10-1.PubMed CentralView ArticlePubMedGoogle Scholar
- Bories G, Caiazzo R, Derudas B, Copin C, Raverdy V, Pigeyre M, Pattou F, Staels B, Chinetti-Gbaguidi G: Impaired alternative macrophage differentiation of peripheral blood mononuclear cells from obese subjects. Diab Vasc Dis Res. 2012, 9: 189-195. 10.1177/1479164111430242.PubMed CentralView ArticlePubMedGoogle Scholar
- Mosser DM, Edwards JP: Exploring the full spectrum of macrophage activation. Nat Rev Immunol. 2008, 8: 958-969. 10.1038/nri2448.PubMed CentralView ArticlePubMedGoogle Scholar
- Thorp EB: Mechanisms of failed apoptotic cell clearance by phagocyte subsets in cardiovascular disease. Apoptosis. 2010, 15: 1124-1136. 10.1007/s10495-010-0516-6.PubMed CentralView ArticlePubMedGoogle Scholar
- Zizzo G, Hilliard BA, Monestier M, Cohen PL: Efficient Clearance of Early Apoptotic Cells by Human Macrophages Requires M2c Polarization and MerTK Induction. J Immunol. 2012, 189: 3508-3520. 10.4049/jimmunol.1200662.PubMed CentralView ArticlePubMedGoogle Scholar
- Gallardo-Soler A, Gomez-Nieto C, Campo ML, Marathe C, Tontonoz P, Castrillo A, Corraliza I: Arginase I induction by modified lipoproteins in macrophages: a peroxisome proliferator-activated receptor-gamma/delta-mediated effect that links lipid metabolism and immunity. Mol Endocrinol. 2008, 22: 1394-1402. 10.1210/me.2007-0525.View ArticlePubMedGoogle Scholar
- Gordon S: Alternative activation of macrophages. Nat Rev Immunol. 2003, 3: 23-35. 10.1038/nri978.View ArticlePubMedGoogle Scholar
- Laskin DL: Macrophages and inflammatory mediators in chemical toxicity: a battle of forces. Chem Res Toxicol. 2009, 22: 1376-1385. 10.1021/tx900086v.PubMed CentralView ArticlePubMedGoogle Scholar
- Porcheray F, Viaud S, Rimaniol AC, Leone C, Samah B, Dereuddre-Bosquet N, Dormont D, Gras G: Macrophage activation switching: an asset for the resolution of inflammation. Clin Exp Immunol. 2005, 142: 481-489.PubMed CentralPubMedGoogle Scholar
- Badylak SF, Valentin JE, Ravindra AK, McCabe GP, Stewart-Akers AM: Macrophage phenotype as a determinant of biologic scaffold remodeling. Tissue Eng Part A. 2008, 14: 1835-1842. 10.1089/ten.tea.2007.0264.View ArticlePubMedGoogle Scholar
- Zheng XF, Hong YX, Feng GJ, Zhang GF, Rogers H, Lewis MA, Williams DW, Xia ZF, Song B, Wei XQ: Lipopolysaccharide-induced M2 to M1 macrophage transformation for IL-12p70 production is blocked by Candida albicans mediated up-regulation of EBI3 expression. PLoS One. 2013, 8: e63967-10.1371/journal.pone.0063967.PubMed CentralView ArticlePubMedGoogle Scholar
- Ohri CM, Shikotra A, Green RH, Waller DA, Bradding P: The tissue microlocalisation and cellular expression of CD163, VEGF, HLA-DR, iNOS, and MRP 8/14 is correlated to clinical outcome in NSCLC. PLoS One. 2011, 6: e21874-10.1371/journal.pone.0021874.PubMed CentralView ArticlePubMedGoogle Scholar
- Hasan D, Chalouhi N, Jabbour P, Hashimoto T: Macrophage imbalance (M1 vs. M2) and upregulation of mast cells in wall of ruptured human cerebral aneurysms: preliminary results. J Neuroinflammation. 2012, 9: 222-10.1186/1742-2094-9-222.PubMed CentralView ArticlePubMedGoogle Scholar
- Belge KU, Dayyani F, Horelt A, Siedlar M, Frankenberger M, Frankenberger B, Espevik T, Ziegler-Heitbrock L: The proinflammatory CD14 + CD16 + DR++ monocytes are a major source of TNF. J Immunol. 2002, 168: 3536-3542.View ArticlePubMedGoogle Scholar
- Kim J, Hematti P: Mesenchymal stem cell-educated macrophages: a novel type of alternatively activated macrophages. Exp Hematol. 2009, 37: 1445-1453. 10.1016/j.exphem.2009.09.004.PubMed CentralView ArticlePubMedGoogle Scholar
- Xu W, Roos A, Schlagwein N, Woltman AM, Daha MR, van Kooten C: IL-10-producing macrophages preferentially clear early apoptotic cells. Blood. 2006, 107: 4930-4937. 10.1182/blood-2005-10-4144.View ArticlePubMedGoogle Scholar
- Bottcher A, Gaipl US, Furnrohr BG, Herrmann M, Girkontaite I, Kalden JR, Voll RE: Involvement of phosphatidylserine, alphavbeta3, CD14, CD36, and complement C1q in the phagocytosis of primary necrotic lymphocytes by macrophages. Arthritis Rheum. 2006, 54: 927-938. 10.1002/art.21660.View ArticlePubMedGoogle Scholar
- Thorp E, Tabas I: Mechanisms and consequences of efferocytosis in advanced atherosclerosis. J Leukoc Biol. 2009, 86: 1089-1095. 10.1189/jlb.0209115.PubMed CentralView ArticlePubMedGoogle Scholar
- Weigert A, Jennewein C, Brune B: The liaison between apoptotic cells and macrophages–the end programs the beginning. Biol Chem. 2009, 390: 379-390.View ArticlePubMedGoogle Scholar
- Martinez FO, Helming L, Gordon S: Alternative activation of macrophages: an immunologic functional perspective. Annu Rev Immunol. 2009, 27: 451-483. 10.1146/annurev.immunol.021908.132532.View ArticlePubMedGoogle Scholar
- Abumaree MH, Al Jumah MA, Kalionis B, Jawdat D, Al Khaldi A, Abomaray FM, Fatani AS, Chamley LW, Knawy BA: Human Placental Mesenchymal Stem Cells (pMSCs) Play a Role as Immune Suppressive Cells by Shifting Macrophage Differentiation from Inflammatory M1 to Anti-inflammatory M2 Macrophages. Stem Cell Rev. 2013, 9: 620-641. 10.1007/s12015-013-9455-2.View ArticlePubMedGoogle Scholar
- Saclier M, Yacoub-Youssef H, Mackey AL, Arnold L, Ardjoune H, Magnan M, Sailhan F, Chelly J, Pavlath GK, Mounier R, Kjaer M, Chazaud B: Differentially activated macrophages orchestrate myogenic precursor cell fate during human skeletal muscle regeneration. Stem Cells. 2013, 31: 384-396. 10.1002/stem.1288.View ArticlePubMedGoogle Scholar
- Shaikh S, Brittenden J, Lahiri R, Brown PA, Thies F, Wilson HM: Macrophage subtypes in symptomatic carotid artery and femoral artery plaques. Eur J Vasc Endovasc Surg. 2012, 44: 491-497. 10.1016/j.ejvs.2012.08.005.View ArticlePubMedGoogle Scholar
- Bartel RL, Booth E, Cramer C, Ledford K, Watling S, Zeigler F: From bench to bedside: review of gene and cell-based therapies and the slow advancement into Phase 3 Clinical Trials, with a focus on Aastrom’s Ixmyelocel-T. Stem Cell Rev. 2013, 9: 373-383. 10.1007/s12015-013-9431-x.PubMed CentralView ArticlePubMedGoogle Scholar
- van Weel V, van Tongeren RB, van Hinsbergh VW, van Bockel JH, Quax PH: Vascular growth in ischemic limbs: a review of mechanisms and possible therapeutic stimulation. Ann Vasc Surg. 2008, 22: 582-597. 10.1016/j.avsg.2008.02.017.View ArticlePubMedGoogle Scholar
- Fadini GP, de Kreutzenberg SV, Boscaro E, Albiero M, Cappellari R, Krankel N, Landmesser U, Toniolo A, Bolego C, Cignarella A, Seeger F, Dimmeler S, Zeiher A, Agostini C, Avogaro A: An unbalanced monocyte polarisation in peripheral blood and bone marrow of patients with type 2 diabetes has an impact on microangiopathy. Diabetologia. 2013, 56: 1856-1866. 10.1007/s00125-013-2918-9.View ArticlePubMedGoogle Scholar
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