Magnetic targeting enhances retrograde cell retention in a rat model of myocardial infarction
© Huang et al.; licensee BioMed Central Ltd. 2013
Received: 1 February 2013
Accepted: 2 December 2013
Published: 12 December 2013
Retrograde coronary venous infusion is a promising delivery method for cellular cardiomyoplasty. Poor cell retention is the major obstacle to the establishment of this method as the preferred route for cell delivery. Here, we explored whether magnetic targeting could enhance retrograde cell retention in a rat model of myocardial infarction.
Rat mesenchymal stem cells were labeled with superparamagnetic oxide nanoparticles. The magnetic responsiveness of MSCs was observed while cells flowed through a tube that served as a model of blood vessels in a 0.6-Tesla magnetic field. In a Sprague–Dawley rat model of acute myocardial infarction, 1 × 106 magnetic mesenchymal stem cells were transjugularly injected into the left cardiac vein while a 0.6-Tesla magnet was placed above the heart. The cardiac retention of transplanted cells was assessed by using quantitative Y chromosome-specific polymerase chain reaction, cardiac magnetic resonance imaging, and optical imaging. Cardiac function was measured by using echocardiography, and histologic analyses of infarct morphology and angiogenesis were obtained.
The flowing iron oxide-labeled mesenchymal stem cells were effectively attracted to the area where the magnet was positioned. Twenty-four hours after cellular retrocoronary delivery, magnetic targeting significantly increased the cardiac retention of transplanted cells by 2.73- to 2.87-fold. Histologic analyses showed that more transplanted cells were distributed in the anterior wall of the left ventricle. The enhanced cell engraftment persisted for at least 3 weeks, at which time, left ventricular remodeling was attenuated, and cardiac function benefit was improved.
These results suggest that magnetic targeting offers new perspectives for retrograde coronary venous delivery to enhance cell retention and subsequent functional benefit in heart diseases.
Cell therapy is a promising approach for acute myocardial infarction (AMI) and heart failure, and its efficacy largely depends on cell homing, retention, and engraftment within the injured myocardium. With unique access to the ischemic myocardium, retrograde coronary venous delivery has been demonstrated to provide efficient cell dissemination in the setting of occluded or diffusely narrowed coronary arteries and has subsequently shown functional benefits in both animal and clinical studies [1–6]. However, compared with the antegrade approach, cell retention using the retrograde intracoronary approach was inferior [7–9]. Poor cell retention is the major obstacle in establishing this method as the preferred route for cell delivery.
In recent years, magnetic targeting strategies traditionally used in chemotherapy for tumors  have been introduced to localize magnetic nanoparticle-loaded cells delivered to in vivo target lesions [11–14]. Until now, magnetic targeting strategies have been successfully introduced to attract cells infused via intramyocardial  and intracoronary [16, 17] routes to the ischemic heart. This technique has been proven to enhance cell retention, engraftment, and functional benefits. However, few data exist on the efficacy of magnetic targeting on retrograde cell retention.
Based on a new transjugular cardiac vein retroinfusion technique  and an analysis of the interaction between a magnet cylinder and the magnetically labeled MSCs, here we explored whether magnetically targeted cell delivery could enhance myocardial retention of MSCs after retrograde coronary vein infusion in a rat model of myocardial infarction.
Methods and materials
A permanent neodymium-iron-boron (NdFeB) magnet cylinder with a diameter of 8 mm (Shanghai Yahao Instrument Equipment Co., China) was used in this study. The magnetic flux density (B) of the magnet surface was up to 600 mT, measured by using a model 51,662 Leybold Tesla meter. The distribution of the magnetic flux density was calculated with finite element analysis.
Preparation of magnetically labeled cells
Bone marrow MSCs were isolated from 4-week-old male Sprague–Dawley (SD) rats weighing 100 to 120 g, as described before [19, 20]. All cells used for the subsequent experiments were harvested with 0.25% trypsin when they reached 80% to 90% confluence at passage 4.
MSCs were labeled with superparamagnetic iron oxide nanoparticles (SPIO; Schering, Berlin, Germany; 100 mg/ml, 62 nm in diameter) and poly-L-lysine (PLL; 0.15 mg/ml), with an iron concentration of 50 μg/ml and a PLL concentration of 0.15 μg/ml . The magnetic SPIO-labeled MSCs (MagMSCs) were then incubated with 1 μM ethyl iodotricarbocyanine iodide (DiR; ABD Bioquest, Inc., California, USA) according to the manufacturer’s protocol. Prussian blue staining and transmission electron microscopy (Philips CM120) were used to evaluate the presence and localization of intracellular iron particles. The iron content in the cells was quantified by using atomic absorption spectrometry (Thermo E.IRIS Duo ICP). Inverted microscopy was used to examine the staining efficacy of the DiR dye.
Proliferation assays and determination of viability
For the proliferation and viability assays, the following conditions were investigated: unlabeled MSCs without exposure to magnetic fields (MSCs), unlabeled MSCs with exposure to magnetic fields (Mag-MSCs), SPIO-labeled MSCs without exposure to magnetic fields (SPIO-MSCs), and SPIO-labeled MSCs with exposure to magnetic fields (Mag-SPIO-MSCs). For the exposure to magnetic fields, the 75-cm2 cultures were positioned above and in direct contact with the magnets for 24 hours.
In the proliferation assays, cells were seeded at 3 × 105 cells/flask, and the medium was changed every 3 days. At subconfluence (90%), the cells were detached with Accutase (PAA Laboratories, Cölbe, Germany) and counted with a CASY2 Analyser (CASY2-Cell Counter and Analyser System, Model TT, Roche Diagnostics, Mannheim, Germany) . The experiments were performed in triplicate. Cellular viability under the different conditions was examined with the help of a CASY2 Analyser, according to the ECE method described by Lindl et al.  as well as the viability-SOP of the manufacturer.
In vitro magnetic capture of flowing MagMSCs
The cell suspension circulated at flow rates of 4 mm/s, 20 mm/s, 100 mm/s, and 500 mm/s to mimic circulatory conditions in animals and human beings. Then the effects of the fluid velocity on the accumulation of MagMSCs were observed. All experiments were performed in triplicate for each condition.
Acute myocardial infarction model
The animal experiments were approved by the Animal Care and Use Committee of Fudan University and were in compliance with the Guide for the Care and Use of Laboratory Animals, published by the National Academy Press (NIH Publication No. 85–23, revised 1996). An experimental anterior-wall MI was induced, as previously described by Antonio et al.. In brief, the rats were anesthetized with ketamine (100 mg/kg, i.p.) and xylazine (10 mg/kg, i.p.), and a rodent ventilator was used to ventilate rats with room air at 80 breaths per minute. The heart was exteriorized through a left thoracotomy, and the left anterior descending coronary artery (LAD) was occluded with a 6–0 polypropylene suture.
Transjugular cardiac vein catheterization and its perfusion area
Half an hour after LAD ligation, a transjugular cardiac vein catheterization was induced under additional anesthesia with ketamine (30 mg/kg, i.p.), as we reported recently . In brief, a flexible wire with a bent end was inserted into the left internal jugular vein and advanced slowly along the left superior vena cava. Under direct vision, the wire was run into the left cardiac vein by rotating the wire and changing the position of its tip. A fine tube was advanced along the wire to the proximal-mid segment of the left cardiac vein. A temporal snare suture was placed at the distal left cardiac vein to prevent flush back of injected solution into the left vena cava.
To examine the perfusion area of the transjugular cardiac vein catheter, 1 ml of 0.4% Evans blue solution was injected slowly and selectively into the left cardiac vein while the snare was tied. Five minutes later, the snare was released to allow reperfusion, and the catheter was removed. The heart was removed after 5 minutes of observation and cut into five segments parallel to the apex-base axis. The perfusion area of the retrograde infusion was compared with the ischemic area induced by LAD ligation. The latter was evaluated with triphenyltetrazolium chloride (TTC) staining.
In vivo magnetic targeting of retrograde delivered MagMSCs
In the Mag group, 20 minutes after the establishment of transjugular cardiac vein access in the AMI model, 1 × 106 Mag-Dir-MSCs were resuspended in 1 ml PBS and injected slowly and selectively into the left cardiac vein while the snare was tied. Five minutes later, the snare was released to allow reperfusion, and the catheter was removed. For magnetic targeting, the cylindrical NdFeB magnet was placed close to the injured myocardium (0 to 1 mm) for 10 minutes during and after the cell injection, which has been suggested to be the optimized time [15, 17]. The surgical wounds were repaired, and the rats were extubated and returned to their cages to recover.
Cardiac magnetic resonance imaging at 24 hours after injection
To determine the efficacy of magnetic targeting, six animals from each group were killed for magnetic resonance imaging (MRI) at 24 hours after the injection of cells or PBS. MRI was performed in a 3.0-T clinical whole-body magnetic resonance scanner by using a 5-cm-diameter four-element phased-array animal coil (MAGNETOM Verio; Siemens Healthcare). Images were acquired by T2 fl2d sequence with a slice thickness of 1.5 mm, a flip angle of 20 degrees, a field of view of 80 mm, a bandwidth of 240, a matrix of 256 × 256, a repetition time of 150 ms, and an echo time of 4.69 ms. After in vivo imaging, the animals were killed. The hearts were explanted for fluorescence examination and then placed in liquid nitrogen and stored at −80°C for further RT-PCR analysis.
Consecutive short-axis slices were acquired to analyze the signal intensity in the myocardium of the left ventricle with Image J software (NIH, Bethesda, MD, USA). The relative signal intensity of the anterior wall was calculated as the signal intensity in the anterior wall divided by the signal intensity in the interventricular septum.
Cardiac fluorescence imaging at 24 hours after injection
After cardiac MRI, the hearts of six animals were explanted for fluorescence imaging. Extensive PBS washing was performed to remove any cells adhering to the epicardium. The hearts were placed in a Carestream In-Vivo Multispectral Imaging System FX PRO to detect DiR fluorescence under 748-nm excitation and 780-nm emission. The exposure time was set at 3 seconds and was maintained during the entire imaging session. Hearts from the PBS group were also imaged as controls for background noise.
Quantification of male-donor MSCs in rat heart
As a complementary means of measuring cell retention, we transplanted male MSCs into female recipient hearts to enable the use of Y chromosome-specific PCR to quantify the exact numbers of cells per milligram of heart tissue . Quantitative PCR for the Sex-determining Region Y gene (SRY) was performed 24 hours (after fluorescence imaging) and 3 weeks after cell infusion. The primers for specific rat Y chromosome DNA are as follows: sense: 5'-GAG CTT TGG GAG CAG TGA C-3', anti-sense: 5'-ATG AGG CTG ATA TTT ATA GTT TGG-3'. Cell retention was calculated as the total number of cells detected in the heart divided by the number of infused cells.
Assessment of cardiac function
Cardiac remodeling and left ventricular function were assessed by transthoracic echocardiography 3 weeks after AMI by using a Vevo 770 high-resolution imaging system (Visual Sonics) with a 17.5-MHz probe. After the induction of light general anesthesia, the hearts were imaged in the two-dimensional and M-modes. The recordings were obtained from the parasternal long-axis view at the level of the greatest LV diameter. The LV internal end-diastolic diameter (LVEDd) and LV internal end-systolic diameter (LVEDs) were measured from M-mode recordings, according to the leading-edge method. All echocardiographic measurements were averaged from at least five separate cardiac cycles. All procedures and analyses were performed by an experienced researcher who was blinded to the groups.
Because vascular delivered cells reportedly translocate into the parenchyma after 48 to 72 hours , the distribution of infused cells within the hearts was analyzed at 72 hours after injection of cells or PBS. Six hearts from each group were stained with Prussian blue, and another six hearts from each group were frozen in OCT for cell fluorescence imaging. For Prussian blue staining, pathological specimens were incubated for 30 minutes with 2% potassium ferrocyanide (Perl reagent) in 6% hydrochloric acid and were counterstained with nuclear fast red.
The representative hearts were harvested and frozen in OCT compound 3 weeks after rat echocardiography. Cryostat sections (8 μm thickness) were obtained for immunofluorescence staining to evaluate cardiomyocyte differentiation of injected MSCs. Antibodies used included troponin T (TnT, cardiac isoform Ab-1; Laboratory Vision Corp., Chicago, IL, USA), as described previously . Capillary density was assessed by staining with anti-CD31 antibody (BD Biosciences, San Diego, CA, USA), as previously described . The sections were visualized by fluorescence-labeled secondary antibodies (Molecular Probes, Inc., Eugene, OR, USA). In total, 10 visual fields where a cross section of capillaries was clearly visible in the peri-infarct zone were randomly selected, and the numbers of capillaries per square millimeter were obtained after superimposing a calibrated morphometric grid on each digital image by using ImageJ Software.
For morphometric analysis, six representative recipient hearts in each group were harvested 3 weeks after functional assessment and were embedded in paraffin. From the Masson’s trichrome-stained images, morphometric parameters including risk area and the LV wall thicknesses in the risk and noninfarcted regions were measured in each section . Images were acquired digitally and analyzed by using the NIH ImageJ software. All analyses were performed in a blinded manner.
The data are expressed as the mean ± SD. The numbers of transplanted cells from the Mag and the NonMag groups were compared by using Student t test. The data from the three groups were compared, and intergroup differences were analyzed with one-way ANOVA (analysis of variance) and the least-significant difference (LSD) test. The relation between the accumulation percentage of MagMSCs and the fluid average velocity was analyzed by the Pearson correlation test. Statistical analyses were performed with SPSS 15.0. A value of P < 0.05 was considered statistically significant.
Magnetic analysis of the NdFeB magnet cylinder
Characterization of MagMSCs
In vitro magnetic capture of flowing MagMSCs
Perfusion area of dye injection via the transjugular cardiac vein in AMI rats
Magnetically enhanced MagMSC retention in the rat heart
After cardiac MR imaging, in vitro fluorescence imaging of extracted hearts was performed. The fluorescence signals in the Mag group were approximately 2.73 times higher than those of the NonMag group (26,222 ± 5,102 versus 9,620 ± 1,930 photon/S; P < 0.001), whereas the signal was 6,155 ± 953 photon/S in the PBS group, which was used as a negative control (Figure 6C). These results reflected that the application of the NdFeB magnet-enhanced cell retention at the target site.
To avoid nonspecific imaging of the cells stained with iron and DiR, quantitative PCR for the male-specific SRY gene was performed at both 24 hours and 3 weeks after cell infusion. The Mag group exhibited a 2.87-fold greater cell-retention rate than the NonMag group at 24 hours after cell infusion (P < 0.001). Three weeks later, both groups experienced a substantial decrease in surviving cells. However, the Mag group still exhibited enhanced cell engraftment relative to the NonMag group (P < 0.001) (Figure 6D).
Distribution of MagMSCs within the rat heart
Morphometric and functional changes
Cellular therapy via retrograde coronary infusion, with the ability to safely deliver large numbers of cells regardless of occluded or diffusely stenotic coronary arteries, offers an attractive option for the treatment of heart diseases. Its feasibility and safety have been demonstrated clinically and experimentally [1–6]. However, the efficiency of retrograde delivery into the heart was considered the lowest when compared with antegrade coronary and intramyocardial injection-based delivery techniques [7–9]. Poor cell retention has been the main hurdle in establishing retrograde coronary infusion as the preferred route for cell delivery. Previous data suggest that poor retention is caused mainly by the rapid loss of a high proportion of cells into the systemic circulation (washing-out effect) within a few minutes [29, 30]. Here, we demonstrated that brief (10 minutes) magnetic attraction successfully attenuates cell loss during injection. The increased cell retention is confirmed by the combination of quantification of the injected cells, Y chromosome-specific PCR, and MRI cell imaging. The use of the SRY marker prevented the mistaken conclusion that the grafted cells were still present, whereas iron released on cell death has been engulfed in macrophages and has subsequently given rise to false-positive signals . Notably, this transient magnetic targeting seemed to have a beneficial effect on subsequent cell-therapy outcomes, and both cell engraftment and functional benefits were enhanced. Even though the magnetic targeting boosts cell engraftment at 24 hours and 3 weeks, the absolute number is still very low. Therefore, we speculated that indirect regeneration mechanisms (for example, paracrine effects) dominate the observed functional benefits.
The present study is the first to introduce magnetically targeted cellular cardiomyoplasty to the retrograde coronary-delivery strategy. Previous investigations of magnetically targeted cell delivery for cardiovascular applications are limited to intramyocardial and antegrade intracoronary routes [15–17]. Magnetic attraction can attenuate cell loss via venous drainage, increase cells’ intravascular stay, and enhance the formation of multicellular clusters, which was considered the theoretic basis for cellular magnetic targeting . However, the magnetically guided cell-mass formation may increase the risk of coronary embolism after antegrade delivery , especially when relatively large cells such as MSCs (approximately 20 to 25 μm in diameter) are used. The more cells accumulated, the greater the risk of embolism became. On the contrary, retrograde intracoronary delivery could avoid this complication while allowing safer access to ischemic myocardium. In addition, our in vitro experiment demonstrated that the number of magnetically accumulated cells was inversely proportional to the flow velocity. Higher cell retention may be reasonably expected from the retrograde venous route than from the antegrade arterial route. Therefore, we speculated that retrograde coronary administration rather than antegrade coronary infusion may serve as an optimal candidate for cellular magnetic targeting. However, further studies are required to verify our reasoning.
Because of the inherent attenuation of the magnetic flux density , a magnet should be positioned near the target site to maximize the targeting effect. During the conventional retrograde coronary delivery in small animals, the direct insertion of a coronary sinus is needed [4, 32]. The counterclockwise rotation of the heart made it technically difficult to put an external magnet simultaneously on the infarcted anterior wall in a rat. In this study, we adopted a new transjugular cardiac vein retroinfusion technique that we recently developed in which cells could be injected via a fine tube inserted into the left jugular vein . In this way, inversion of the rat heart was no longer needed, and the magnet could therefore be easily positioned on the target site of the infarcted anterior wall.
An inconsistency in territory occurred between the arterial branches and the venous branches , and the territory of the left cardiac vein is not identical to that of the LAD. However, the perfusion via the left cardiac vein can cover the infarcted myocardium, as shown in this study; this is considered to be the theoretic basis of retrograde cellular therapy for LAD-related AMI . Moreover, the infarcted tissue lacks blood flow and mechanical contraction, which decreases the loss of transplanted cells by means of washing out and squeezing out . This finding could explain the relative large numbers of cells transplanted in the left ventricular anterior wall in our study, even without an external magnet. Based on this observation, an external magnet with a surface area similar to the infarct zone that was superpositioned to the injured tissue further enhanced the cell retention in the infarcted anterior wall.
The data concerning the optimal dosage for retrograde cellular therapy are limited, especially in small animals such as rats. The reported retrograde dosages of skeletal muscle precursor cells rather than MSCs varied as widely as 1 × 106[4, 32], 5 × 106, and 2 × 106 cells/100 g body weight . The dosage of MSCs used in our study was 1 × 106, which was the usual dosage used in antegrade coronary delivery in rats and was identical to the number of skeletal muscle precursor cells reported by Suzuki [4, 32]. However, relatively large numbers of cells may be acceptable because retrograde intracoronary delivery bears minimal risk of coronary embolism. Although the magnetic technique could reduce cell dosage once the optimal dosage for therapeutic cell therapy is agreed in a specific model, further studies aimed at clarifying the cell dose-effect relation and identifying the optimal dose for retrograde cell infusion with or without magnetic targeting are warranted.
Some limitations of the present study exist. First, we did not explore the detailed relation between different magnetic intensities and targeting efficacy; further studies are warranted to identify the optimal magnetic intensity and to determine the targeting duration at which the optimal retention and distribution of donor cells is achieved. Second, because the retrograde coronary delivery commonly adopts a nonsurgical catheter-based technique in human beings , a spatially focused magnetic targeting strategy recently proposed by us [36, 37] will be required for the translation of our results to a clinical setting. Third, this study failed to clarify the kinetics of cell extravasation in different time after cell infusion. Until now, the data concerning MSCs extravasation with venous delivery are limited and should be explored in future studies. Finally, because of the inability of the fluorescence devices available for performing in vivo imaging in rats, cell-retention dynamics were not analyzed to minimize the deaths of the animals.
This study highlights that the cell retention and subsequent functional benefits via retrograde coronary infusion can be significantly enhanced by magnetic targeting in a rat model of myocardial infarction. Additional bench work is warranted to optimize targeting parameters and to assess the relevance of this approach in a clinically relevant large-animal model.
Acute myocardial infarction
magnetic flux density
magnetic attraction force on MagMSCs in the r direction
left anterior descending coronary artery
left ventricular end-diastolic dimension
left ventricular end-systolic dimension
left ventricular ejection fraction
left ventricular fractional shortening
magnetic SPIO-labeled MSCs
magnetic resonance imaging
mesenchymal stem cell
superparamagnetic iron oxide nanoparticles
region Y gene
The project was funded by National Natural Science Fundation of China (81370003, 81000043, 11304194, and 81100145), Research Fund for the Doctoral Program of Higher Education of China (20100071120072), Natural Science Foundation of Shanghai Municipality of China (11ZR1406300), and Central Universities Basic Research Funds for the Young Teachers Research Capacity Enhancement Projects of China (20520133291).
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