Open Access

Cell culture models for study of differentiated adipose cells

Stem Cell Research & Therapy20145:137

DOI: 10.1186/scrt527

Published: 16 December 2014

Abstract

Adipose cells are an important source of mesenchymal stem cells and are important for direct use in research on lipid metabolism and obesity. In addition to use of primary cultures, there is increasing interest in other sources of larger numbers of cells, using approaches including induced pluripotent stem cell differentiation and viral immortalisation.

There is increasing interest in the use of adipose cells, both brown and white types, not least because the obesity epidemic dictates a need for increased research on adipose tissue and lipid metabolism. The paper by Balducci and colleagues – a collaboration between five Italian groups – reports the immortalisation, by lentiviral transduction, of human adipose-derived stromal cells [1].

Adipose cells are an important resource for biomedical research, partly because of the ready availability of human surgical material and the fact that they can be used to generate mesenchymal stem cells [2] – which themselves have interesting differentiation potential, for example towards a hepatocyte phenotype [3] – and even pluripotent stem cells [4]. Adipose-derived stem cells have been reported to differentiate into osteoblasts, chondrocytes, myocytes and neurons, as well as back to adipocytes, depending on the culture conditions [5]. Pluripotent stem cells [6, 7], including induced pluripotent stem cells [8], can be differentiated in vitro into cells with multiple phenotypic characteristics of adipose cells, including specifically brown adipose cells [7].

It is interesting to note that telomerase expression in bone marrow stromal cells resulted in enhanced bone formation [9, 10]. Another approach to generating large populations of adipose cells in vitro is to use viral immortalisation, as has been also achieved in other systems such as bone marrow progenitor cells [11].

Balducci and colleagues report diversity in differentiation potential between cell lines immortalised with different gene combinations, which is in itself an interesting observation, but it is not entirely clear what the cellular or molecular basis for this may be or whether it is a purely random observation [1]. Whatever the mechanism, human adipose-derived stromal cells co-transduced with human telomerase reverse transcriptase and human papilloma virus E6/E7 generated immortalised cells that retained the capacity to differentiate down osteogenic and adipogenic lineages and to produce angiogenesis-related proteins. Cells transduced with human telomerase reverse transcriptase alone or with human telomerase reverse transcriptase and Simian virus 40 did not retain these capacities to the same extent. The availability of immortal cell lines that closely resemble adipose-derived stromal cells contributes a useful new resource for those working on this fascinating cell type.

Nevertheless, it is important to bear in mind that, as in all such cases, these adipose cells are not normal cells identical to their parental finite-lifespan progenitors – transduced cells are unlikely to be acceptable for therapeutic use except perhaps in the terminal stages of life-threatening diseases. Indeed, Balducci and colleagues acknowledge this limitation and report on chromosomal aberrations and unbalanced translocations in the transduced cells. However, there is no doubt that the availability of these immortalised human adipose-derived stromal cell lines will significantly facilitate research on this interesting and relatively neglected cell type, and availability of these cell lines will help to answer more rapidly questions about their biology and expedite their application in cell therapy/tissue engineering, even if the cells eventually used for therapy will most probably be of primary origin rather than cell lines. The availability of large numbers of these cells that can be easily grown also offers the potential for discovery of additional autocrine, paracrine and endocrine factors which these cells may produce, but at levels too low to be detected from small-scale, limited-lifespan primary cultures, and this, in the end, could be the most valuable legacy from this interesting paper.

The availability of these different sources of adipose cells provides a much-expanded toolkit for research on adipose cells in vitro, and should make a significant impact on the progress of obesity research and on our understanding of adipose cell differentiation.

Declarations

Authors’ Affiliations

(1)
National Institute for Cellular Biotechnology, Dublin City University

References

  1. Balducci L, Blasi A, Saldarelli M, Soleti A, Pessina A, Bonomi A, Coccè V, Dossena M, Tosetti V, Ceserani V, Navone SE, Falchetti ML, Parati EA, Alessandri G: Immortalization of human adipose-derived stromal cells: production of cell lines with high growth rate, mesenchymal marker expression and capability to secrete high levels of angiogenic factors. Stem Cell Res Ther. 2014, 5: 63-10.1186/scrt452.PubMed CentralView ArticlePubMedGoogle Scholar
  2. Romagnoli C, Brandi ML: Adipose mesenchymal stem cells in the field of bone tissue engineering. World J Stem Cells. 2014, 6: 144-152. 10.4252/wjsc.v6.i2.144.PubMed CentralView ArticlePubMedGoogle Scholar
  3. Li X, Yuan J, Li W, Liu S, Hua M, Lu X, Zhang H: Direct differentiation of homogeneous human adipose stem cells into functional hepatocytes by mimicking liver embryogenesis. J Cell Physiol. 2014, 229: 801-812. 10.1002/jcp.24501.View ArticlePubMedGoogle Scholar
  4. Jumabay M, Abdmaulen R, Ly A, Cobberly MR, Shahmirian LJ, Heydarkhan-Hagvall S, Dumesic DA, Yao Y, Bostrom KI: Pluripotent stem cells derived from mouse and human white mature adipocytes. Stem Cell Transl Med. 2014, 3: 161-171. 10.5966/sctm.2013-0107.View ArticleGoogle Scholar
  5. Kim EH, Heo CY: Current applications of adipose-derived stem cells and their future perspectives. World J Stem Cells. 2014, 6: 65-68. 10.4252/wjsc.v6.i1.65.PubMed CentralView ArticlePubMedGoogle Scholar
  6. Song X, Li Y, Chen X, Yin G, Huang Q, Chen Y, Xu G, Wang L: bFGF promotes adipocyte differentiation in human mesenchymal stem cells derived from embryonic stem cells. Genet Mol Biol. 2014, 37: 127-134. 10.1590/S1415-47572014000100019.PubMed CentralView ArticlePubMedGoogle Scholar
  7. Cuaranta-Monroy I, Simandi Z, Kolostyak Z, Doan-Xuan QM, Poliska S, Horath A, Nagg Y, Basco Z, Nagy L: Highly efficient differentiation of embryonic stem cells into adipocytes by ascorbic acid. Stem Cell Res. 2014, 13: 88-97. 10.1016/j.scr.2014.04.015.View ArticlePubMedGoogle Scholar
  8. Nischio M, Saeki K: Differentiation of human pluripotent stem cells into highly functional classical brown adipocytes. Methods Enzymol. 2014, 537: 177-197.View ArticleGoogle Scholar
  9. Shi S, Gronthos S, Chen S, Reddi A, Counter CM, Robey PG, Wang CY: Bone formation by human postnatal bone marrow stromal stem cells is enhanced by telomerase expression. Nat Biotechnol. 2002, 20: 587-591. 10.1038/nbt0602-587.View ArticlePubMedGoogle Scholar
  10. Simonsen JL, Rosada C, Serakinci N, Justesen J, Stenderup K, Rattan SI, Jensen TG, Kassem M: Telomerase expression extends the proliferative life-span and maintains the osteogenic potential of human bone marrow stromal cells. Nat Biotechnol. 2002, 20: 592-596. 10.1038/nbt0602-592.View ArticlePubMedGoogle Scholar
  11. Osyczka AM, Nöth U, O’Connor J, Caterson EJ, Yoon K, Danielson KG, Tuan RS: Multilineage differentiation of adult human bone marrow progenitor cells transduced with human papilloma virus type 16 E6/E7 genes. Calif Tissue Int. 2002, 71: 447-458. 10.1007/s00223-001-1090-2.View ArticleGoogle Scholar

Copyright

© Clynes; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. The licensee has exclusive rights to distribute this article, in any medium, for 12 months following its publication. After this time, the article is available under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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