Fig. 5
MSCs promote the efferocytosis and migration of LPMs to the injured uteri to repair the injured endometria. (A) Schematic of in vitro coculture system setup. (B, C) Efferocytosis of LPMs from each group was determined by (B) flow cytometry and (C) confocal microscope (scale bar: 50 μm). (D) Crystal violet staining of LPMs cocultured with MSCs or LPS (scale bar: 100 μm). (E) Schematic of in vivo experimental model design (n = 5–7). (F) Flow cytometry analysis for LPMs isolated from peritoneal cavity harvested after 7-days injury. Cells were pregated on CD11b+. (G) Flow cytometry analysis for LPMs isolated from uteri harvested after 7-days injury. (H) Flow cytometry analysis for GPX4 expression of CD11b+F4/80med subsets in the uteri harvested after 7-days injury. (I) HE, Masson’s trichrome staining, MDA, ER, and PR stainings of endometrial tissues obtained from mice of three groups. Scale bar indicates 100 μm. (J) The mRNA expression levels of TNF-α, IL-1β, and IL-6 in the endometria of mice were determined by qRT-PCR (normalised to β-actin). (K) Serum concentrations of TNF-α, IL-1β, and IL-6 were measured by ELISA. Values are mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ns denotes p > 0.05 (by unpaired Student’s t test)