Figure 3From: Metastatic neuroblastoma cancer stem cells exhibit flexible plasticity and adaptive stemness signaling Modulations in cell proliferation levels of MSDACs under alternated culture conditions. A) Representative microphotographs of random fields obtained from Operetta high-content confocal imaging showing BrdU incorporation levels in three different clones of MSDACs maintained in serum-free stem cell medium for three generations: SF-SCM-1(3G), SF-SCM-1(3G) cells transferred and maintained in growth medium with FBS for three generations (SF-SCM-1(3G) → GM-FBS(3G), or the MSDACs reintroduced in SF-SCM for three generations (SF-SCM-1(3G) → GM-FBS(3G) → SF-SCM-2(3G). Parental SH-SY5Y cells maintained in growth medium are used as controls. B) Histograms obtained from Columbus image analysis showing significant decline in BrdU positive cells in SF-SCM-1(3G) → GM-FBS(3G) MSDACs and regained and amplified cell proliferation in MSDACs reintroduced in SF-SCM (SF-SCM-1(3G) → GM-FBS(3G) → SF-SCM-2(3G). Brd-U, bromodeoxyuridine; MSDACs, metastatic site-derived aggressive cells.Back to article page