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Figure 3 | Stem Cell Research & Therapy

Figure 3

From: Identification and characterization of epithelial cells derived from human ovarian follicular fluid

Figure 3

Larger oocyte-like structures developed spontaneously from epithelial cells derived from human follicular fluid. (A) The volume of primary epithelial cells increased after 2 weeks of culturing. (B) Prominent nucleus and perinuclear accumulation of cytoplasmic organelles were present in large oocyte-like cells. Different sizes of oocyte-like structures after they were detached by trypsin after (C) 2 weeks , (D) 3 weeks , and (E) 4 weeks of culturing. (F) Blastocyst-like structures stained with 4′,6-diamidino-2-phenylindole developed after 6 weeks of culturing (arrowhead). Zona pellucida-like structures (arrow) were observed in epithelial cells post culture, indicating different stages of oocyte-like structure maturation. Scale bars: 200 μm (A), 100 μm (B, C), and 50 μm (D, E, F).

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