Figure 5
From: Identification and characterization of epithelial cells derived from human ovarian follicular fluid
Characterization of oocyte-like structures observed post culture of epithelial cells by immunolocalization of germ cell markers. The oocyte-like structures stained positive after 4 weeks culturing for DAZL, ZPC, STELLA, SCP, and GDF-9, respectively. All markers are specific to ooplasm. 4′,6-Diamidino-2-phenylindole (DAPI) was used to counterstain and observe the nuclei. Scale bar = 50 μm.