Comparison of the
differentiation potential of human embryonic stem cell and induced pluripotent stem cell lines. (A) Quantitative PCR analysis of genes related to three germ layers, germ cells, and trophoblasts in human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) following in vitro differentiation. The iPSCs (iCFB46, iCFB50, iGRA1, iGRA2, and iGRA7) and hESCs (H9) were examined at day 20 of in vitro differentiation. Relative gene expression was first normalized to that of GAPDH, and then represented as the fold change relative to H9. Values represent the mean ± standard deviation (SD) (n = 3). Statistical analysis for lineage-specific gene expression is presented in Additional file 3. (B) Immunofluorescence staining against CDX2 and EOMES on day 7 of trophoblastic differentiation of representative H9, iCFB50, and iGRA1 cells. Scale bars = 50 μm. (C) Quantitative analysis of CDX2 and EOMES positive cells from day 7 of trophoblastic differentiation of H9, iCFB50, and iGRA1, iGRA2, and iGRA7. Approximately 600 cells were counted for each experiment. Values are means ± SD (n = 3). *P <0.05, **P <0.01, ***P <0.001.