Figure 5

Microarray-based analysis of global gene expression in multiple human embryonic stem cell and induced pluripotent stem cell clones. (A) Hierarchical clustering of global expression profiles of human embryonic stem cells (hESCs) (H9 and NTU1) and induced pluripotent stem cells (iPSCs) (iCFB46, iCFB50, iDPC3, iDPC4, iGRA1, and iGRA2). (B) Scatter plots of relative gene expression between hESCs and iPSCs (hESC to iCFB, hESC to iDPC, and hESC to iGRA). Each dot represents the averaged expression level of one gene in different samples. Green lines, fourfold changes between two samples; purple arrow, low expression level of the NLRP2 gene in iGRAs as compared with hESCs. (C) Heat map showing the expression levels of NLRP family members in hESCs, iPSCs, and their parental cells. Sex-related genes were excluded during microarray analysis to avoid sex bias between hESCs (H9 and NTU1 are both 46, XX) and iPSCs.